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Towing tank tests in calm water were performed on a trimaran planing hull to verify its navigational properties with different displacements and centres of gravity, as well as to assess the effects of air jets and bilge keels on the hull’s planing capabilities, and to increase the longitudinal stability of the hull. Hydrostatic roll tests, zero speed tests, and sea trials in the presence of regular waves were conducted to investigate the hull’s seakeeping ability. The test results indicate that the influence of the location of the centre of gravity on the hull resistance is similar to that of a normal trimaran planing hull; namely, moving the centre of gravity backward will reduce the resistance but lower the stability. Bilge keels improve the longitudinal stability but slightly affect the resistance, and the presence of air jets in the hull’s channels decreases the trim angle and increases heaving but has little effect on the resistance. Frequent small-angle rolling occurs in waves. The heaving and pitching motions peak at the encounter frequency of , and the peaks increase with velocity and move towards greater encounter frequencies. When the encounter frequency exceeds, the hull motion decreases, which leads to changes in the navigation speed and frequency
The remediation of heavy metal-contaminated sites using plants is a promising alternative to current methodologies. In this study, small-scale wetlands were constructed to search for new plant species that are suitable and hold potential for phytoremediation of heavy metalcontaminated wastewater originating from an electroplating plant. Ten macrophyte species [Phragmites australis (Cav.) Trin., Typha orientalis Presl, Lythrum salicaria Linn., Arundo donax Linn. var. versicolor Stokes, Typha minima Funk, Juncus effusus L., Pontederia cordata L., Cyperus alternifolius Linn. subsp. flabelliformis (Rottb.) Ku¨kenth., Acorus calamus Linn., and Iris pseudacorus Linn.] were investigated and compared for their shapes, biomass, roots, and ability to accumulate heavy metals. Acorus calamus Linn., T. orientalis Presl, P. australis (Cav.) Trin., T. minima Funk, and L. salicaria Linn. exhibited the highest levels of metal tolerance, whereas P. cordata L., I. pseudacorus Linn., and C. alternifolius Linn. subsp. flabelliformis (Rottb.) Ku¨kenth. had the lowest. Some plants accumulated higher concentrations of metals in the tissues compared with other species such as T. minima Funk, P. australis (Cav.) Trin., L. salicaria Linn., A. donax Linn. var. versicolor Stokes, P. cordata L., and A. calamus Linn., whereas T. orientalis Presl and C. alternifolius Linn. subsp. flabelliformis (Rottb.) Ku¨kenth. had poor capacity to accumulate heavy metals. The results showed that, of the 10 species, P. australis (Cav.) Trin., A. calamus Linn., T. minima Funk, and L. salicaria Linn. are the most suitable and promising plant materials for phytoremediation of heavy metal-contaminated wastewater.
In order to identify high-speed navigation ability of trimaran planing hull, as well as investigate the characteristics of its resistance and hull form, ship model tests were conducted to measure resistance, trim and heaving under different displacements and gravity centre locations. The test results were then used to study the influence of spray strips on resistance and sea-keeping qualities. Moreover, different planing surfaces were compared in the model tests which helped to look into influence of steps on hull resistance and its moving position. Also, the resistance features of monohull and trimaran planing hulls, both with and without steps, were compared to each other. From the tests it can be concluded that: the two auxiliary side hulls increase aerodynamic lift at high-speed motion, which improves the hydrodynamic performance; the trimaran planing hull has also excellent longitudinal stability and low wave-making action; when Fr∇ > 8, its motion is still stable and two distinct resistance peaks and two changes of sailing state (the second change is smaller) appear; spray strips are favourable for sea-keeping qualities at high speed. The change trends before the second resistance peak as to the resistance and sailing behaviour of trimaran planing hull without steps are the same as for monohull planing hull without steps. but when steps in both hulls exist the change trends are different; more specifically: trimaran planing hull with steps has only one resistance peak and its resistance increases along with its speed increasing, and the resistance is improved at the increasing speed as the number of steps increases
Recent studies suggest that horizontal gene transfer (HGT) played a significant role in the evolution of eukaryotic lineages. We here review the mechanisms of HGT in plants and the importance of HGT in land plant evolution. In particular, we discuss the role of HGT in plant colonization of land, phototropic response, C4 photosynthesis, and mitochondrial genome evolution.
This study investigates the influence of initial pH on anodic biofilm formation of microbial electrolysis cells. Initial pH affects the activities of microorganisms in MEC, and furthermore will affect hydrogen (H₂) generation. Therefore, we explore the effects of initial pH on anodic biofilm formation of microbial electrolysis cells and the intrinsic reasons. In a single-chamber MEC, with different initial pH (which are 5.0, 6.0, 7.0, 8.0, and 9.0), the maximum power density during the enrichment process was 2.73 mA/cm² at pH 8.0, which is 56% and 23% higher than those working at pH 7.0 and 9.0, and get worse under acidic conditions. pH 8.0 also showed the highest coulombic efficiency of 46.4% compared with other experimental groups, and energy recovery efficiency is 17.5%. Membrane biomass, as an indicator for anode microbial biomass, decreased sharply at pH 5.0 and 6.0 compared with the neutral and alkaline. Scanning electron microscopy verifies that alkalescent conditions are beneficial to form more rod-shaped bacteria in MECs. These results show that electrochemical interactions between bacteria and electrodes in MECs are enhanced under neutral and alkaline conditions, and the optimal initial pH for anode bacteria formation of 8.0. The information provided below will be useful for improving MEC hydrogen generation.
High-resolution measurements of rainfall, water level, pH, conductivity, temperature, [K⁺], [NO₃⁻] and [Ca²⁺] of the Landiantang epikarst spring at Nongla, Mashan County in Guangxi Province, China were recorded by using data loggers with a time interval of fifteen minutes. The results showed that the pH of the Landiantang Spring dropped and the conductivity fell as well. As Ca²⁺, Mg²⁺ and HCO₃⁻ were the dominant ions, the linear relationships between conductivity and those ions were developed to calculate variations in SIC, SID and LogPCO2 of the spring during rainfalls. The LogPCO2 of Landiantang Spring during rainfalls was lower than that at lower flows, and its SIC and SID also were lower. It can be figured out that the dilution of precipitation controls the hydrochemical variations of Landiantang Spring during rainfall, and the water-rock-gas interactions control the hydrochemical variations of the spring at the usual time. The process of water-rockgas is universal to Landiantang Spring because after rainfall, gas with high CO₂ concentration dissolves in water flowing as spring, which in turn becomes more highly undersaturated, dissolves more dolomite to make up for the effect of precipitation dilution, and the conductivity renews slowly after rainfall. However, to explain the hydrological and chemical changes, the dilution of precipitation may be more important during rainfall, because it is the key process to controlling the chemical evolutions of the spring. The [K⁺] and [NO₃⁻] rise rapidly as the [Ca²⁺] falls during rainfall. Therefore, an important conclusion is hypothesized that the restricted growth of plants in karst regions is possibly caused not only by the low labile trace elements in soil, but also by the loss of these nutritional elements in the ecosystem. Moreover, fertilizers, for example, can also be brought away through the epikarst zone by flowing water due to high fissure and permeability of the epikarst zone, which will contaminate epikarst spring and groundwater, and may produce serious environmental problems. Thus, how to develop effective solutions to karst water-related environmental challenges will become the primary study of karst aquifers and water resources in the future.
Taking advantage of coastal wave environment to carry out scaled ship model test is an effective testing technology for ship performance. In this paper, the method of spectral analysis is adopted to calculate the significant wave height, period, wave spectrum and some other parameters of some places along the North Sea area of China. The measured wave spectrum and the ocean spectrum are handled into non-dimensional form to evaluate their similarity. The influence of wind direction and tide on coastal waves was analyzed. And the results indicate that the coastal wave spectrum is similar to the ocean spectrum under some specific conditions
Newly discovered specimens of the orthothecide hyoliths in Hebei Province of North China reveal new data on diversity, skeletal microstructure and palaeoecology of the order Orthothecida. Decoritheca cyrene possesses a well-defined skeletal structure consisting of rows of tubercles in the shell wall, which correspond to dimples on the internal surface of the conch. We describe a new species Cupitheca convexa sp. nov. characterised by a planar and interior convex operculum with a pair of bilobate cardinal processes, which differs from the more widespread C. holocyclata in opercular morphology and overall ornamentation of the conch, highting the significance of operculum in the classification of hyoliths. First discovery of the presumed unusual protoconch of C. convexa sp. nov. implies a possible lecithotrophic development to adapt to nutrient-fluctuant environments. Pitting structure on the operculum and shell as well as lamellar- fibrillar structure on the internal mould of C. convexa sp. nov. are observed herein.
As an essential micronutrient, iron (Fe) is directly involved in several fundamental processes in the photosynthetic cells. However, it is not clear if photosynthetic traits affected by high ferrous level are associated with changes in fatty acid composition in chloroplast membranes. To accomplish this, the effects of excess Fe2+ on the fatty acid composition and the fluidity properties of the chloroplast membrane, photosynthesis rate and the chlorophyll fluorescence were investigated in pea (Pisum sativum L.) seedlings grown hydroponically in nutrient solutions with 100, 200, 400 and 600 lM Fe2+ supplied as FeSO4. Increased fluidity of the chloroplast membranes was found under higher Fe2+ treatments, and this might be attributed to the increase in relative content of unsaturated fatty acids (USFA). Excess Fe2+ decreased the chlorophyll content and the electron transport rate, deactivated reaction center of photosystem II, and declined plant net photosynthetic rate. Finally, the reduced plant dry weight was observed. The results indicate that the effects of excess Fe on photosynthesis and fluidity of chloroplast membrane depend on the stress strength and duration, and the increased fluidity of chloroplast membrane may be critical in maintenance of cellular integrity under excess but not lethiferous Fe2+ treatment.
We investigated substrate material and the effects of different fertilizers and water levels as variable factors for slope restoration. A field rainfall monitoring experiment was carried out to explore morphological changes in Amorpha fruticosa L., the water-holding capacity of its leaves under different water and nutrient gradients, and the nutrient losses from the substrate. The results showed that nutrient loss by runoff was significantly affected by fertilizer use and they increased with the increased application of fertilizers. The concentration of nitrogen in runoff was insignificant, the concentration of phosphorus was increased, and the runoff concentration of potassium was decreased after increasing water-retaining agent levels in the substrate. The concentration of nutrients in runoff from rainfall generally followed a trend of slight fluctuations, then a rise, and finally a decrease. The addition of F2 fertilizer produced the lowest nitrogen losses from substrate material and reduced the leaf area where the addition of phosphate fertilizer had a significant effect on crown diameter. At greater content of the water-retaining agent, the water in substrate material increased, resulting in increased water absorption by the plants and increased relative water content of leaves. Ultimately, W3F4 was the most favorable combination of water-retaining agent and fertilizer concentration for plant growth, which may be related to runoff losses. This combination provides optimal conditions under which the plant can maintain a perfect balance of nutrients and thus improve plant growth indices.
An experiment was carried out to compare cadmium and copper absorption and mineral nutrition accumulation in pennisetum and tall fescue in order to select an appropriate grass to remediate Cd/Cu-contaminated soil and explore their detoxification mechanisms of contamination by mineral elements. The biomass remained constant in tall fescue under each Cd addition level and increased in pennisetum until Cu reached 500 μM, whereas they dramatically decreased as the Cu or Cd solution increased, which was concurrent with quadratic regression model analysis. The Cd/Cu concentrations in tall fescue were mostly accumulated in the roots and were much higher than those in pennisetum. The extracted amount of Cd in the shoots and the total Cu concentrations of pennisetum were higher than the corresponding values in tall fescue at every Cd/Cu addition level. Negative correlations were observed between Cd and shoot Ca, Cu, K, Mg, and Zn, and root Cu and Na of tall fescue and the root K of pennisetum. The Cu concentration was negatively correlated with K and positively correlated with Na in tall fescue and pennisetum under the Cu treatments. As the Cd/Cu concentration in solution increased, K/Na values were significantly decreased in the roots of tall fescue under Cu stress and pennisetum under Cd/Cu stress, whereas they increased in the roots of tall fescue under Cd addition. In summary, pennisetum exhibited the greater biomass and Cd/Cu extraction; indicating it as a candidate energy grass for phytoextraction. The adjustment capacity of grass for K and Na might relate to the tolerance to Cd/Cu.
The membrane motor protein, prestin, encoded by gene Prestin, provides the electromotility to cochlear outer hair cells (OHCs) and is therefore considered responsible for cochlea's high sensitivity to sound waves. Echolocating bats use ultrasound for orientation and hunting. In this study, we obtained the complete Prestin coding sequences of 60 mammal taxa. Alignment results detected the same 3-bp deletion mutation (c.1833_1835del in exon 18) in two bat lineages, Pteropodidae and Emballonuroidea, and caused an amino acid deletion (p.Asp611del). These two bat lineages orient with vision and echolocation, separately, suggesting that the parallel deletion occurred independently after their split. Homology modeling of the protein structures indicated contrasting structural variations after the deletion of this amino acid. Estimation of the distributions of the surface electrostatic potential coincided with the structural variation. Our findings suggest the complexity of the echolocation in bats but functionality analyses are needed to illuminate it.
The issue of controlling a swarm of autonomous unmanned surface vehicles (USVs) in a practical maritime environment is studied in this paper. A hierarchical control framework associated with control algorithms for the USV swarm is proposed. In order to implement the distributed control of the autonomous swarm, the control framework is divided into three task layers. The first layer is the tele-operated task layer, which delivers the human operator’s command to the remote USV swarm. The second layer deals with autonomous tasks (i.e. swarm dispersion, or avoidance of obstacles and/or inner-USV collisions), which are defined by specific mathematical functions. The third layer is the control allocation layer, in which the control inputs are designed by applying the sliding mode control method. The motion controller is proved asymptotically stable by using the Lyapunov method. Numerical simulation of USV swarm motion is used to verify the effectiveness of the control framework
Pear (Pyrus communis L.) is a nutrient-dense fruit with strong consumer demand and high commercial value. However, most cultivated pear varieties are often susceptible to diseases caused by fungi, bacteria, and viruses. The purpose of the present study was to establish an efficient genetic transformation and regeneration protocol, paving the way for genetic engineering of pear cultivars with enhanced disease resistance. Major factors that influence transformation and regeneration were examined and optimal conditions were established for efficient transformation from leaf explants of ‘Old Home’, a valuable pear interstem and rootstock. High transformation efficiency was achieved largely due to an improved infection/transformation induction strategy. Co-cultivation of Agrobacterium cells and leaf segments on a liquid induction medium yielded a fivefold increase in transformation frequency. Southern hybridization analysis revealed presence of reporter gene uidA in the genomic DNA samples from independent transgenic plants, confirming the integration of the transgene in recipient pear genomes. The stability of T-DNA integration was evaluated by the consistent presence of the Km selectable marker and the expression pattern of the introduced reporter gene uidA was analyzed by GUS histochemical assay.
Auxin has been widely implicated in various aspects of plant growth and development, including flower development. In order to further elucidate the role of auxin during flower development, especially on the pistil development process, auxin response factors (ARFs), an important component in auxin signalling pathway, were studied in the early flower buds of Japanese apricot (Prunus mume Sieb. et Zucc). In this study, a comprehensive overview of the ARF gene family in Japanese apricot is presented, including the chromosomal locations, phylogenetic relationships, gene structures, the domain and nuclear localization analysis. Seventeen Japanese apricot genes that encode ARF proteins (PmARFs) have been identified based on the genome sequence of Japanese apricot. Comparison of the expression of some PmARF genes between perfect and imperfect flower buds in Japanese apricot suggests that PmARFs, especially the PmARF13 and PmARF17 gene may be required for pistil development and function in Japanese apricot. These results will be useful for future functional analyses of the ARF family genes in plants.
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