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1

Prawo i epidemiologia włośnicy w Polsce a możliwości hodowli świń w warunkach kontrolowanych

100%
Rozycki M., Chmurzynska E., Bilska-Zajac E.
Weterynaria w Terenie
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2016
|
tom 10
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nr 1
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Aktualne problemy związane z Anisakis simplex - pasożytem ryb morskich

100%
Bilska-Zajac E., Rozycki M., Chmurzynska E., Osek J.
Życie Weterynaryjne
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2012
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tom 87
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nr 02
3
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Identyfikacja gatunkowości mięsa, podstawy prawne oraz przegląd metod badań

88%
Rozycki M., Chmurzynska E., Bilska-Zajac E., Karamon J., Cencek T.
Życie Weterynaryjne
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2016
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tom 91
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nr 08
4

Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat

88%
Rozycki M., Chmurzynska E., Bilska-Zajac E., Karamon J., Cencek T.
Journal of Veterinary Research
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2018
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tom 62
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nr 2
5

Wyniki badań biegłości (PT) w kierunku włośni w 2014 r.

88%
Chmurzynska E., Rozycki M., Bilska-Zajac E., Karamon J., Cencek T.
Medycyna Weterynaryjna
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2016
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tom 72
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nr 05
The aim of this article is to present and analyze the obtained PT results for the last year (i.e. 2014). The material for PT was the properly minced pork meat (or horse meat for horse slaughterhouses) spiked with Trichinella larvae. Live Trichinella larvae were obtained during the digestion process. Meat samples weighting 50 g were spiked under microscopic control with a known number of larvae preserved with gelatin gels of the appropriate concentration. In 2014 over 250 laboratories routinely examining pig meat took part in PT. On the level of 5 larvae 4 laboratories obtained doubtful results and were 9 + inconsistent in quantitative analysis. The average value of the z-scores was 0.84. The qualitative assessment of PT results on the level of contamination 3, 5 larvae and negative (blank) samples showed 21, 9 and 4 of unsatisfactory results. At the limit of detection – 1 larva per sample – positive results were obtained by 87.8% of laboratories. In the same year 14 laboratories testing horse meat participated in PT – all of them obtained consistent results. The average value of the z-score was 0.88. In a study in 2014 257 laboratories participated. This is comparable to the number of participants relative to the years 2012 and 2013. The quantitative assessment average z-score was 0.84 and it was lower than in previous years (which means better). The percentage of consistent results in qualitative research in previous years (2009–2011) was a bit higher and ranged from 91.6% to almost 95%. In 2014 the quality of PT has not deteriorated compared to previous years despite a three times smaller number of larvae in samples. It should be emphasized that the organization of the PT scheme permitted additionally assessing the laboratories by examination of the samples at the limit of detection of the method.
6
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Rola dochodzeń epidemiologicznych w aktualnej sytuacji epidemiologicznej włośnicy w Polsce

88%
Bilska-Zajac E., Rozycki M., Karamon J., Sroka J., Cencek T.
Życie Weterynaryjne
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2019
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tom 94
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nr 06
7
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Alaria alata jako potencjalne zagrożenie zdrowia ludzi - występowanie i rozpoznawanie

88%
Chmurzynska E., Rozycki M., Bilska-Zajac E., Karamon J., Cencek T.
Życie Weterynaryjne
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2013
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tom 88
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nr 09
8

Prevalence of tick-borne encephalitis virus antibodies in domestic and game animals from Easten Poland

76%
Cisak E., Wojcik-Fatla A., Sroka J., Zajac W., Bilska-Zajac E., Chmurzynska E., Dutkiewicz J.
Bulletin of the Veterinary Institute in Puławy
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2012
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tom 56
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nr 3
Serum samples from 123 cattle, 95 wild boars, and 43 deer (red deer, roe deer, and fallow deer) from the territory of eastern Poland were examined by the ELISA for the presence of specific antibodies against tick-borne encephalitis virus (TBEV). The rates of positive response in the animals were 4.1%, 16.8%, and 11.6%, respectively. Examination of 37 blood samples from deer with RT-PCR revealed only one positive result in a roe deer (2.7%). The relatively high serologic response rate in wild boars was due to a very high response rate (35.7%) in the Chełm district, which accounted for 94% of the total positive results. These findings seem to indicate that the Chełm district is most probably an endemic area of TBEV.
9
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Uczulenia ludzi na alergeny Anisakis simplex

76%
Kochanowski M., Karamon J., Dabrowska J., Cencek T., Bilska-Zajac E., Rozycki M.
Życie Weterynaryjne
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2013
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tom 88
|
nr 08
10
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Echinococcus multilocularis w Polsce - sytuacja epizootyczna u lisów wskaźnikiem ryzyka zarażenia ludzi

76%
Karamon J., Kochanowski M., Dabrowska J., Rozycki M., Bilska-Zajac E., Sroka J., Cencek T.
Życie Weterynaryjne
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2015
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tom 90
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nr 09
11

Intestinal helminths of raccoon dogs (Nyctereutes procyonoides) and red foxes (Vulpes vulpes) from the Augustow Primeval Forest (north-eastern Poland)

76%
Karamon J., Samorek-Pierog M., Moskwa B., Rozycki J., Bilska-Zajac E., Zdybel J., Wlodarczyk M.
Journal of Veterinary Research
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2016
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tom 60
|
nr 3
12

Dynamics of Echinococcus multilocularis infection in red fox populations with high and low prevalence of this parasite in Poland (2007–2014)

76%
Karamon J., Kochanowski M., Dabrowska J., Sroka J., Rozycki M., Bilska-Zajac E., Cencek T.
Bulletin of the Veterinary Institute in Puławy
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2015
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tom 59
|
nr 2
The aim of the study was to estimate the current prevalence of E. multilocularis in selected populations of red foxes in Poland and to evaluate the changes in prevalence of this parasite by comparison with the results obtained in the same area during earlier surveillance. The investigations were performed in the area of four Polish provinces: 2 eastern/south-eastern (Lubelskie and Podkarpackie) and 2 south-western (Śląskie and Opolskie). Five hundred red foxes coming from the investigated areas were examined between 2013 and 2014 to estimate the current situation in selected provinces. Moreover, 550 red foxes from the same areas examined between 2007 and 2013 were used for comparison of differences in E. multilocularis prevalences in time. Intestines were examined with the use of the sedimentation and counting technique. Among 500 foxes examined in the current study, 118 were positive for E. multilocularis. There were differences in prevalence between individual provinces: Podkarpackie Province - 54.6%, Lubelskie Province - 18.9%, Śląskie Province - 11.7%, and Opolskie Province - 3.9%. Statistical analysis demonstrated that in most cases there were no differences in prevalence between the current results and the results from previous studies. Only in Opolskie Province was a statistically significant increase observed between 2010 and 2014. A stable degree of infection in the region with high prevalence of this parasite was demonstrated. However, a significant increase in the region with very low prevalence of E. multilocularis points out the necessity to monitor this infection during the coming years to control the progress of the disease.
13
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Development of a sandwich ELISA to detect Anisakis simplex antigens in fish products

76%
Kochanowski M., Rozycki M., Karamon J., Bilska-Zajac E., Dabrowska J., Antolak E., Cencek T.
Annals of Parasitology
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2016
|
tom 62
|
nr Suppl.
14
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Włośnica w Polsce północno‑zachodniej. Czy parazytoza wymyka się spod kontroli?

76%
Rozycki M., Kubica M., Bilska-Zajac E., Chmurzynska E., Karamon J., Cencek T.
Życie Weterynaryjne
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2016
|
tom 91
|
nr 05
15

Metody wykrywania Anisakis simplex w rybach i produktach rybnych

64%
Kochanowski M., Rozycki M., Dabrowska J., Bilska-Zajac E., Karamon J., Antolak E., Gradziel-Krukowska K., Cencek T.
Medycyna Weterynaryjna
|
2018
|
tom 74
|
nr 04
Anisakis simplex is a zoonotic nematode which can cause human anisakiasis. Furthermore, A. simplex allergens, even of dead larvae, can cause allergic reactions, including anaphylaxis. Due to the frequent occurrence in fish muscles and pathogenicity, A. simplex is a serious danger for consumers of fish products. Therefore, it is necessary to examine fish and fish products for the presence of these parasites before placing them on the market. The purpose of this paper is the review of the methods for A. simplex detection in fish and fishery products. These methods differ according to their effectiveness and type of the target analyte. They also have different suitability for examination of matrices with different properties. Moreover, this paper presents legislation associated with A. simplex detection.
16
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Toxoplasma gondii infection in selected species of free-living animals in Poland

64%
Sroka J., Karamon J., Wojcik-Fatla A., Dutkiewicz J., Bilska-Zajac E., Zajac V., Piotrowska W., Cencek T.
Annals of Agricultural and Environmental Medicine
|
2019
|
tom 26
|
nr 4
17

Optimisation and comparison of three PCR procedures for molecular identification of Taenia solium

64%
Karamon J., Sroka J., Cecnek T., Rozycki M., Chmurzynska E., Bilska-Zajac E., Zdybel J., Nowak P., Kedzierska J., Debiak P.
Bulletin of the Veterinary Institute in Puławy
|
2013
|
tom 57
|
nr 4
The aim of the study was to optimise selected PCR methods for identification of T. solium, and to compare their effectiveness and usefulness. The investigation concerned three PCR methods described earlier: PCR I (specific to oncosphere- specific protein Tso31 gene), PCR II (specific to large subunit rRNA gene), and PCR III (cytochrome c oxidases ubunit 1 gene). Each of them needed optimisation in connection with some changes in the procedures. Among the examined procedures, PCR I was found to be the most useful, requiring the least corrections during optimisation - only a higher concentration of polymerase was necessary. Testing an optimised PCR II method showed strong unspecific reactions with E. granulosus and T. saginata. This method was not considered diagnostically useful in distinguishing 7. solium. PCR III method yielded products only when annealing temperature was lowered by 2°C. Under such conditions, there were no unspecific reactions with three others Taenidae parasites; however, annealing at a temperature only 1°C lower generated a distinct unspecific PCR product from T. saginata DNA. Therefore, this method was of limited usefulness. Comparison of the effectiveness of the two selected methods (PCR I and III) in detection of T. solium in successive DNA dilutions showed a large difference between them: in the same DNA sample, PCR I showed positive results in a sample diluted 1:3200, while PCR III failed at dilutions greater than 1:50. The results showed that among the three different methods used in the investigations, the most specific and effective for identification of 7. solium was PCR I.
18
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Study on prevalence of Toxoplasma gondii infection in free-living animals in Poland

64%
Sroka J., Karamon J., Bilska-Zajac E., Stojecki K., Rozycki M., Kusyk P., Wojcik-Fatla A., Zajac V., Cencek T.
Annals of Parasitology
|
2016
|
tom 62
|
nr Suppl.
19

First record of wild boar infected with Trichinella pseudospiralis in Poland

64%
Bilska-Zajac E., Rozycki M., Chmurzynska E., Karamon J., Sroka J., Antolak E., Prochniak M., Cencek T.
Journal of Veterinary Research
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2016
|
tom 60
|
nr 2
20
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The changing epidemiological situation of Echinococcus multilocularis in red foxes in Poland

64%
Karamon J., Samorek-Pierog M., Kochanowski M., Dabrowska J., Rozycki M., Bilska-Zajac E., Chmurzynska E., Sroka J., Cencek T.
Annals of Parasitology
|
2016
|
tom 62
|
nr Suppl.
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