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The structure of the ovule of Sida hermaphrodita (L.) Rusby after pollination

100%
Chudzik B., Szczuka E., Domaciuk M., Pavlov D.
Acta Agrobotanica
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2010
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tom 63
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nr 2
The anatomical structure and the distribution of arabinogalactan proteins were investigated in the ovule of an energetic plant Sida hermaphrodita (L.) Rusby after pollination. Material was collected from an experimental field of the University of Life Sciences in Lublin (Felin). After embedding for immunoflurescence, semi-thin sections were stained with toluidine blue or incubated with JIM 13 or MAC 207 monoclonal antibodies for immunolabeling of arabinogalactan proteins. The structure of the ovule showed some differences in comparison to other representatives of the Malvaceae family. Conversely, the distribution of arabinogalactan proteins did not differ significantly from their distribution in the ovules of other angiosperms at the investigated stage of development.
2

Estimation of chemical composition, net energy and protein value of lucerne by NIRS and regression equation based of the days of vegetation or the accumulated temperature

100%
Atanassova S., Pavlov D., Todorov N.
Journal of Animal and Feed Sciences
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1994
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tom 03
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nr 4
325-335
3

Alteration of oxidative stress markers and behavior of rats in a novel model of depression

76%
Gorlova A., Pavlov D., Zubkov E., Zorkina Y., Inozemtsev A., Morozova A., Chekhonin V.
Acta Neurobiologiae Experimentalis
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2019
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tom 79
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nr 3
Emotional stress is considered a serious pathogenetic factor of depression. In this study an ultrasound model of emotional stress developed in our laboratory was applied. It is characterized by the use of ultrasound as the stressor agent. Animals are triggered not by any organic or physical disturbances but by the perception of adverse information. This type of stress can induce depressive‑like behavioral changes in rodents, manifested by decreased sucrose preference and increased time of immobility in a forced swim test. Ultrasound stress also increased the levels of oxidative stress markers. This is important, as stress has an established association with increased oxidative processes in the central nervous system. Total glutathione and carbonyl protein content were selected as relevant brain markers, as glutathione plays a critical role in cellular defensive mechanisms during oxidative stress and the level of protein carbonyls can be a measure of global protein oxidation. We demonstrated that two weeks of chronic exposure to ultrasound was enough to cause depressive‑like behavioral changes in rats. Increased levels of oxidative stress markers in the hippocampus and prefrontal cortex were also observed after two weeks of such stress. The current study has two goals: the first is to study the relationship of depression and oxidative stress; the second is an additional validation of our approach to modeling stress‑induced depressive‑like states in rats. The present data further support the validity of the ultrasound model by expanding information related to the influence of ultrasound stress on behavioral and physiological parameters, which are of great importance in the development of stress‑induced depression. A time correlation between the onset of symptoms and a change in the level of oxidative stress markers in the brain is also demonstrated.
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