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Effect of dried pumpkin (Cucurbita maxima D.) supplementation on growth performance, serum biochemistry and parameters antioxidant status of rats. The aim of the studies was to determine the effect of dried pumpkin, used in the diets for rats on parameters of growth, nutrient metabolism and antioxidant status of the animals. The experiment was carried out for 7 weeks with 30 growing male Wistar rats. The animals were classified into three groups, 10 individuals in each group, with the initial body weight of 108 g. The control group (G-0) was fed the semi-synthetic mixture without dried pumpkin additive whereas the experimental groups received the mixture with 5-% (G-5) and 10-% (G-10) additive of the dried pumpkin, Ambar variety. The dry substance was obtained from disintegrated fruits, deprived of seed nests, dried at temperature of 60°C. During the experiment weight gains and feed intake were controlled. After termination of the experiment, the rats were killed by anaesthesia; the blood samples were collected and biochemical indices and indicators of antioxidant status were determined. The dietary treatments had no effects on animal growth and feed utilization. In the animals receiving dried pumpkin in their diets (G-5, G-10) significantly lower level of glucose concentration in serum was found. In group G-0, the higher concentration of triacylglycerolsin relation to group G-10 was recorded. Also, the concentration of total cholesterol in group G-0 was higher in comparison to groups G-5 and G-10. In group G-0, VLDL concentration was also higher in relation to group G-10. In group G-10 vs. groups G-5 and G-0, the higher activity of glutathione peroxidise (GPx) was recorded. Total antioxidant status (TAS) was higher in group G-10 in comparison to groups G-0 and G-5. The effect of the administered diet on indicators of the degree of lipid oxidation was also found. In group G-10 vs. group G-0, thiobarbituric acid reactive substances (TBARS) concentration was lower.
Effect of ensiling pumpkin Cucurbita maxima with the addition of inoculant or without it on chemical composition and quality of silages. The aim of the studies was to determine the effect of ensiling the pumpkin on chemical composition and quality of silages. The silages were produced from pumpkin of Justynka variety. Before ensiling, the disintegrated pumpkin fruits were mixed with the dried beet pulp in ratio equals 80 : 20. Two variants of silages were prepared: with the inoculant and without it. In the silages, the following basic chemical composition was determined: the content of dry matter, crude ash, crude protein, crude fat, crude fibre, NDF, ADF and ADL. The indicators, being an evidence of the run of ensiling process and the quality of the obtained silages, were also determined, i.e. pH, lactic, acetic and butyric acids, ammonia nitrogen, ethanol and aerobic stability. In the obtained silages, as compared to the material before ensiling, the lower content of crude fibre and ADF was found whereas in the silage with inoculant, NDF level was also lowered. The silages with the inoculant were characterized by higher content of lactic and acetic acids and lower level of nitrogen, ammonia and ethanol. The silages with the inoculant had also higher aerobic stability. The conducted studies indicate that the application of inoculant has affected the improvement of the quality of the obtained silages.
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