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The reversibility of anoestrus caused by long-acting GnRH analogue (deslorelin), and the effectiveness of GnRH analogue to induce standing oestrous, and in consequence to the retardation of breeding season, was investigated. During the transition period, 26 fat tailed breed ewes were randomly divided into 3 groups. GnRH analogue Ovuplant and Suprelorin were inserted under the neck skin in group I (n=7) and group II, respectively. No administration was applied in group III (control group, n=10). Suprelorin was removed 60 d after the implant's insertion. The initiation of sexual activities based on the day of progesterone increase, and the first standing oestrous were 12.9±1.86, 34.7±9.69, 81.9±6.31, and 27.3±1.94, 48.3±9.24, 96.9±5.76 in control, Ovuplant and Suprelorin was administered to groups; respectively (P<0.001). In conclusion, deslorelin administration resulted in reversible anoestrus in ewes. During the 60 d experimental period, Suprelorin effectively suppressed sexual activity; therefore, Suprelorin could be used to delay the breeding season.
The effects of levamisole, as an anthelmintic or immunomodulator, on pregnancy were investigated in ewes. Immunomodulatory and anthelmintic doses of levamisole and physiologic saline were injected on days 0 (oestrus), 7, 14, and 21 in group 1 (immunomodulatory dose; n=7), group II (anthelmintic dose; n=6), and group III (control; n=6), respectively. The first service pregnancy rate, tended to be lower (P<0.06) in group I (28.6%) compared to that of groups II (83.3%) and III (83.3%). Plasma progesterone (P₄) concentration was significantly higher (P<0.05) in group II compared to that of group III on day 3, and there was a treatment x day interaction (P<0.05). No effect of levamisole treatments on plasma P₄ concentrations was detected once pregnancy was established. There was a numeric decline in plasma cholesterol concentrations in group II, compared to group I or III on day 3. In conclusion, levamisole as an immunomodulator is detrimental for the establishment of pregnancy, possibly by stimulating general and intrauterine immunity.
The aims of this study were to compare two methods of estrus synchronization and to evaluate the effectiveness of the PMSG treatment combined with P4 application. Fifty non-lactating seasonal anestrus fat-tailed ewes were randomly assigned into five groups. The controlled internal drug release devices (CIDR) were applied during day 14 in group I and in group II. Progesterone impregnated sponges were applied during day 14 in group III and in group IV. And then 500 IU PMSG was injected in group I and III i.m. intravaginal devices removed. Ewes in group V served as controls. There was no difference between the groups in the peak value of LH and LH surge. Although LH surge was seen in the control groups 5 sheep, none of the control ewes expressed estrus. Different progestagen treatments have no different results when they are evaluated in terms of the success of the estrus synchronization. PMSG application, after P4 treatment, increased the success of the synchronization.
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