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Two trials were conducted to investigate the effect of a combination of essential oils (CEO) along with fumarate on in vitro rumen fermentation. In trial 1, the essential oil (EO) from thyme, oregano, cinnamon and lemon were mixed at five different ratios. The CEO were applied at levels of 0-500 mg/l. Addition of CEO decreased gas, methane, total volatile fatty acid (VFA) production at 24 h incubation in a dose-dependent manner. Methane tended to decrease much more than total VFA and gas at the same EO level. The mixture of oils at an equal ratio at 500 mg/l that decreased methane much more than VFA was chosen as the optimal combination. In trial 2, the optimal combination was used with 0, 5, 10 or 15 mmol/l of monosodium fumarate. Addition of fumarate further decreased methane production, with 10 mmol/l fumarate resulting in the largest reduction (80.2%) and the smallest decrease in total VFA (5.7%) and gas production (16.7%). Quantification of several ruminal microbe populations by RT-PCR showed that the optimal combination sharply decreased ruminal protozoa; the populations of fungi and fibrolytic bacteria were also decreased. In summary, at an appropriate level, CEO can inhibit methane production. Inclusion of fumarate can further decrease it, which is attributed mainly to inhibition of protozoa and methanogens.
Our objective was to identify combinations of irrigation and fertiliser application that promoted the photosynthetic productivity and water use efficiency (WUE) of Catalpa bungei. Using response surface methodology based on a rotatable central composite design, we conducted a pot experiment with C. bungei clone 004-1 seedlings. The experiment quantified the effects of interactions between soil water content (W), nitrogen application rate (N), and phosphorus application rate (P) on leaf net photosynthetic rates (Pn), SPAD values (SPAD-502 meter readings of leaf chlorophyll), and carbon isotope composition (δ13C). N had significantly positive effects on the all three leaf parameters, while W had significantly negative effects on leaf δ13C signatures but positive effects on leaf Pn and SPAD values. The magnitude of the N effect exceeded that of the W effect. P had no significant effects on any of the three leaf parameters. The W × N interaction had significant positive effects on Pn and SPAD, but significant negative effects on leaf δ13C. Thus, irrigation and nitrogen application had different effects on seedling growth and WUE, and the two effects were interactive. Catalpa bungei growth was promoted by appropriately increasing nitrogen application and irrigation, but WUE was improved by increasing N and decreasing irrigation. Therefore, strategies for the irrigation and fertilisation of C. bungei plantations should be designed to appropriately balance plant growth and WUE.
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