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1

Identification and antimicrobial susceptibility of microbial agents of otitis externa in dogs

100%
Kahya Demirbilek S., Yilmaz O.
Medycyna Weterynaryjna
|
2019
|
tom 75
|
nr 02
In this study, a total of 277 unmedicated dogs with otitis externa were used. Overall, 413 agents were isolated from 277 ear swab samples; 52.7% of the cases were mono-infections (146 cases), and 37.1% of the cases were poly-infections (103 cases). In 10.1% (28) of the cases, neither bacteria nor yeasts were isolated. Coagulase-positive Staphylococcus spp. were the most frequently isolated bacteria and were found in 90 (21.8%) of the samples. Fifty-eight samples, (14%) were positive for Staphylococcus aureus, 51 (12.3%) for Pseudomonas aeruginosa, 27 (6.5%) for Proteus mirabilis, 27 (6.5%) for Malassezia pachydermatis, 21 (5%) for Corynebacterium spp., 21 (5%) for β-haemolytic Streptococcus spp., 15 (3.6%) for Staphylococcus pseudointermedius, 12 (2.9%) for Proteus spp., 12 (2.9%) for Escherichia coli, 9 (2.1%) for Acinetobacter calcoaceticus, 7 (1.6%) for Trichophyton mentagrophytes, 5 (1.2%) for Staphylococcus auricularis, and 46 (11.1%) for different bacteria and yeasts. A total of 14 different bacteria and yeasts were isolated and identified. Kirby-Bauer antibiotic susceptibility testing was carried out for 10 different antibiotics. The bacterial isolates were found to be resistant to amoxicillin-clavulanic acid (45%), gentamycin (28%), ampicillin/cloxacillin (69%), tobramycin (28%), amikacin (23%), enrofloxacin (47%), chloramphenicol (58%), doxycycline (65%), lincomycin/spectinomycin (58%) and polymyxin B (62%). In conclusion, it is important to test the antimicrobial sensitivity of aetiological agents of otitis externa before treatment so as to prevent the development of antibiotic resistance in bacteria and yeasts.
2

Tracheobronchoscopic, cytological and microbiological results of tracheal and bronchial collapse in dogs

41%
Salci H., Cetin M., Kahya Demirbilek S., Akkoc A., Yilmaz O., Canatan U., Cesme H., Aslan V., Altinci C., Khider M., Yilmaz Z., Bayram A. S.
Medycyna Weterynaryjna
|
2018
|
tom 74
|
nr 02
The aim of this study was to evaluate the tracheobronchoscopic, cytological and microbiological results of tracheal and bronchial collapse in dogs. In total, 8 dogs were included in the study. Clinically, tracheal palpations of the dogs were reflective of tracheal disease, and all dogs coughed on tracheal palpation. Vital parameters and hematological values of the dogs were within the normal ranges. Radiological views of the respiratory tracts and thorax were largely normal, but distinctive tracheal contours were noted in cases 3 and 6. Tracheobronchoscopy was performed under general anesthesia, and endoscopic findings (mucosal surfaces and color, prominent appearance of vessels, chondral ring abnormalities of the trachea, and the presence of bronchial and tracheal collapse) were scored. Bronchoalveolar lavage (BAL) was performed to collect samples for cytological and microbiological analysis. Five cases had tracheal collapse, and two cases had right bronchial collapse. Concurrent tracheal and right bronchial collapse were diagnosed in one case. Cytological results were not indicative of inflammation or infection, but Escherichia coli was isolated from case 2 (bronchial collapse) and case 3 (tracheal collapse). Antibiotic susceptibility results revealed that the organisms were susceptible to sulfamethoxazole/trimethoprim. Statistically, there were no significant differences between the cases in terms of total endoscopic scores. In conclusion, tracheal and/or bronchial collapse should only be diagnosed by tracheobronchoscopic examination. Cytological and microbiological analyses of the BAL fluid in these cases do not always provide valuable data for clinical practitioners.
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