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Effectiveness of chemical agents in removing platinum from DNA isolated from cisplatin-treated HL-60 cells

100%

Olinski R., Briggs R. C., Basinger M., Jones M. M.

Acta Biochimica Polonica

1992

tom 39

nr 4

Human promyelocytic leukemia cells,. HL-60, were treated with cisplattn [cis-diamminedichloroplatinum (II)] (2 itiM, 1 h). DNA- eisplatin-protein complexes were isolated and exposed to thiourea (1M), NaCN (100 mM), diethyldithio-carbamate (500 mM), or tf-methyl-D- glucamine dithiocarbamate (500 mM) for 12 h. The release of platinum was measured by atomic absorption spectroscopy. Sodium cyanide was the most effective agent, releasing about 90% of the DNA-bound platinum. Thiourea was the least effective agent, while dithiocar- bamates exhibited an intermediate. The ability of the same group of agents to split the proteins off from the protein-cisplatin-DNA complex was also evaluated and similarly dithiocarbamate were also the most effective.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Basinger M.

1 Briggs R. C.

1 Jones M. M.

1 Olinski R.

1 1992

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Effectiveness of chemical agents in removing platinum from DNA isolated from cisplatin-treated HL-60 cells