Czestosc wystepowania genow ermA, ermB, ermC i msrA-B u metycylino-opornych klinicznych szczepow Staphylococcus aureus opornych na erytromycyne izolowanych w Polsce

• Autorzy: Mlynarczyk G. , Mlynarczyk A. , Szymanek K. , Luczak M.

Warianty tytułu

EN

The frequency of the occurrence of genes ermA, ermB, ermC and msrA-B among methicillin-resistant Staphylococcus aureus strains resistant to erythromycin

• Języki publikacji: PL

Abstrakty

PL

Przebadano 50 szczepów MRSA(mcthiciIlin resistant Staphylococcus aureus) izolowanych z materiałów od chorych, wykazujących oporność na erytromycynę, na obecność genów erniA, ermB, ermC, msrA/B, przy zastosowaniu PCR i określono częstość występowania tych genów, a także określono, czy oporność ma charakter indukcyjny czy konstytutywny.

EN

The group of 50 clinical MRSA strains resistant to MLS-B was examined for the presence of errnA, enuB, ermC, msrA/B genes by using PCR. Gene ermA was found in 43 strains (86%). 20 oferm A strains demonstrated inducible whereas 23 constitutive type of expression. The gene ermC was present in 15 of examined MRSA strains (30%). The expression of the gene was inducible in the case of 9 and constitutive in the case of б of the strains. The msrA/B gene was present in the case of 5 strains (10%). The ermB gene was not detected among the investigated strains.

Słowa kluczowe

PL

szczepy bakteryjne; Staphylococcus aureus; opornosc na erytromycyne; szczepy metycylinooporne; gen ermA; gen ermB; gen ermC; gen msrA-B; wystepowanie; lancuchowa reakcja polimerazy

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2006
• Tom: 58
• Numer: 3
• Opis fizyczny: s.183-190,tab.,bibliogr.

Twórcy

autor

Mlynarczyk G.

• Akademia Medyczna w Warszawie, ul.Chalubinskiego 5, 02-004 Warszawa

autor

Mlynarczyk A.

autor

Szymanek K.

autor

Luczak M.

Bibliografia

• 1. Bozdogan B, Berrezouga L, Kuo MS i inni. A new resistance gene, linB, conferring resistance to lincosamides by nucleotidylation in Enterococcus faecium HM1025. Antimicrob Agents Chemother 1999; 43: 925-9.
• 2. Haroche J, Morvan A, Davi M i inni. Clonal diversity among streptogramin A-resistant Staphylococcus aureus isolates collected in French hospitals. J Clin Microbiol 2003; 4: 586-91.
• 3. Huang J, О 'Toole PW, Shen W i inni. Novel chromosomally encoded multidrug efflux transporter MdeA in Staphylococcus aureus. Antimicrob Agents Chemother 2004; 48: 909-17.
• 4. Lina G, Quaglia A, Reverdy ME i inni. Distribution of genes encoding resistance to macrolides, lincosamides and streptogramins among staphylococci. Antimicrob Agents Chemother 1999; 43: 1062-106.
• 5. Loeza-Lara PD, Soto-Huipe M, Baizabal-Aguirre VM i inni. pBMSal, a plasmid from a dairy cow isolate of Staphylococcus aureus, encodes a lincomycin resistance determinant and replicates by the rolling-circle mechanism. Plasmid. 2004; 52: 48-56.
• 6. Luna VA, Heiken M, Judge K i inni. Distribution of mef(A) in gram-positive bacteria from healthy Portuguese children. Antimicrob Agents Chemother 2002; 46: 2513-7.
• 7. Malbruny B, Canu A, Bozdogan В i inni. Resistance to quinupristin-dalfopristin due to mutation of L22 ribosomal protein in Staphylococcus aureus. Antimicrob Agents Chemother 2002; 46: 2200-7.
• 8. Martineau F, Picard FJ, Lansac N i inni. Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother 2000; 44: 231-8.
• 9. Matsuoka M, Endou K, Kobayashi H i inni. A dyadic plasmid that shows MLS and PMS resistance in Staphylococcus aureus. FEMS Microbiol Lett 1997; 148: 91-6.
• 10. Matsuoka M, Janosi L, Endou K, Nakajima Y Cloning and sequences of inducible and constitutive macrolide resistance genes in Staphylococcus aureus that correspond to an ABC transporter. FEMS Microbiol Lett 1999; 181: 91-100.
• 11. Mukhtar TA, Koteva KP, Hughes D W Wright GD. Vgb from Staphylococcus aureus inactivates streptogramin В antibiotics by an elimination mechanism not hydrolysis. Biochemistry 2001; 40: 8877-86.
• 12. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: Approved standard M7-A7. National Committee for Clinical Laboratory Standards, Wayne, Pa. 2006.
• 13. Prunier AL, Trong HN, Tande D i inni. Mutation of L4 ribosomal protein conferring unusual macrolide resistance in two independent clinical isolates of Staphylococcus aureus. Microb Drug Resist 2005; 11: 18-20.
• 14. Reynolds E, Ross Jl, Cove JH. Msr(A) and related macrolide/streptogramin resistance determinants: incomplete transporters? Int J Antimicrob Agents 2003; 22: 228-36
• 15. Roberts MC, Sutdiffe J, Courvalin P i inni. Nomenclature for macrolide-lincosamide-streptogramin В resistance determinants. Antimicrob Agents Chemother 1999; 43: 2823-30.
• 16. Schmitz FJ, Petridou J, Fluit A C i inni. Distribution of macrolide-resistance genes m Staphylococcus aureus blood-culture isolates from fifteen German university hospitals. M A.R.S. Study Group. Multicentre Study on Antibiotic Resistance in Staphylococci. Eur J Clin Microbiol Infect Dis 2000; 19: 385-7.
• 17. Schmitz FJ, Sadurski R, Kray A i inni. Prevalence of macrolide-resistance genes in Staphylococcus aureus and Enterococcus faecium isolates from 24 European university hospitals. J Antimicrob Chemother 2000; 45: 891-4.
• 18. Steward CD, Raney PM, Morrell AK i inni. Testing for induction of clindamycin resistance in erythromycin-resistant isolates of Staphylococcus aureus. J Clin Microbiol 2005; 43: 1716-21.
• 19. Sutcliffe J, Grebe T, Tait-Kamradt A, Wondrack L. Detection of erythromycin-resistant determinants by PCR. Antimicrob Agents Chemother, 1996; 40: 2562-6.
• 20. Wondrack L, Massa M, YangBV Sutcliffe J. Clinical strain of Staphylococcus aureus inactivates and causes efflux of macrolides. Antimicrob Agents Chemother 1996; 40: 992-8.