Immunological effects of environmental exposure to NO2 and NO. Results of our study

• Autorzy: Rutowski J. , Moszczynski P. , Dobrowolski J. , Krochmal D.
• Języki publikacji: EN

Abstrakty

EN

The immunological effects of the environmental and/or occupational exposure to NO2 and NO in air as polluting gases have been examined in groups of 16 men. The determina-tion of NO2 and NO concentrations in ambient air in the work enviroment as well as in ambient air in residential areas of these men was performed, always by use of an Amaya-Sugiura passive sampling spectrophotometric method. Mean concentration of NO2 in ambient air in residential areas was 0.0210 mg x m-3 (0.0070 to 0.0470). NO2and NO mean concentrations in ambient air in the work enviroment were 0.0867 mg x m-3 (0.0165 to 0.1960) and 0.0614 mg x m-3 (0.0220 to 0.1090) respectively. For the determination of T-cell and (CD19+)B-cells populations Behring monoclonal antibodies were used in indirect immuno-fluores-cence tests. The serum levels of immunoglobulins: G, A, M, E; C3c and C4 complement components; total circulating immunological complexes (CIC) as well as acute phase proteins: C-reactive protein (CRP), haptoglobin, ceruloplasmin and transferrin were determined by nephelometry. Stimulation T-cell line in exposed to NO2 and NO was evidenced by in-creased number of (CD3+)T-cells, by about twice (p<0.001) increased number of (CD4+)T-helper cells and by increased number of (CD8+)T-suppressor cells. The higher increase in count of (CD4+)T-helper cells than (CD8+)T-suppressor cells population caused the increased value of the (CD+4)T-helper/(CD8+)T-suppressor ratio by about 25% (p<0.01) in the men exposed to NO2 and NO. No changes were observed in the number of (CD19+)B-cells as well as in the (CD3+)T/(CD8+)T-suppressor ratio. In men of exposed to NO2 and NO elevation of IgG serum concentration by a 17.7% (p<0.01) was evidenced as well as decreased of C3c by 18.6% (p<0.001) and C4 by 35% (p<0.001), whereas total CIC in serum was elevated by about twice (p<0.001). Significant positive correlations between concentrations of NO2 in air and numbers of total lymphocytes, (CD3+)-cells, (CD4+)T-helper, (CD8+)T-suppressor cells or IgG (/r/ from 0.31 to 0.71) as well as significant negative correlations between concentrations in air of NO2 and IgE, C3c, CRP or haptoglobin (/r/ from -0.49 to -0.31) were calculated. Moreover, significant positive correlation between NO concentrations in air in work place and counts of (CD3+)T-, (CD8+)T-suppressor, (CD19+)B-cells and levels in serum of C4, haptoglobin and ceruloplasmin (/r/ from 0.33 to 0.63) as well as significant negative correlations between NO concentrations in air in work place and serum levels of IgG, IgA and IgM (/r/ from -0.67 to -0.47) were also observed. In conclusion, environmental exposure to NO2 and NO can modificate in the peripheral blood of humans the parameters of cell-mediated and/or humoral immunity.

Słowa kluczowe

EN

occupational exposure; environmental exposure; immunity; nitric oxide; environment; Nitrous dioxide

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 1998
• Tom: 07
• Numer: 4
• Opis fizyczny: p.245-250,ref.

Twórcy

autor

Rutowski J.

• Polish Academy of Medicine in Warsaw, Department in Tarnow, 178A Lwowska Street, 33-100 Tarnow, Poland

autor

Moszczynski P.

autor

Dobrowolski J.

autor

Krochmal D.

Bibliografia

• 1.BADR O., PROBERT S.D. Appl. Energy 44, 197, 1993.
• 2.United Nations Environmental Programme, Environmental Data Report, 1995/1996, The Alden Press, Oxford, 1997.
• 3.The Environment in Europe: A Global Perspective, National Institute of Public Health and Environmental Protection, RIVM, Bilthoven, 1992.
• 4.ROSSI N.J. Pollut. Eng. 27, 50, 1995.
• 5.EWETZ L. Scand. J. Work. Environ. Health, 19, 21, 1993.
• 6.GUSTAFSSON L.E. Scand. J. Work. Environ. Health 19, 44, 1993.
• 7.BODY S.C., HARTIGAN P.M. Resp. Care Clin. N. Am. 3, 411, 1997.
• 8.AIMASHEVA N.P., ZENINA T.A., MANUKHINA E.B., MI- KOIAN V.D., KUBRINA L.N., VANIN A.F., MALYSHEV LIu. Izv. Akad Nauk Ser. Biol. 5, 634, 1997.
• 9.DELLINGER R.P. ZIMMERMAN J.L. TAYLOR R.W., STRAUBE R.C., HAUSNER D.L., CRINER G.J., DAVIS Jr K., HYERS T.M., PAPADAKOS P. Crit. Care Med. 26, 15, 1998.
• 10.MONCADA S., PALMER R.M.J., HIGGS E.A. Pharmacol. Rev. 43, 109, 1991.
• 11.FEELISCH M., OSTROWSKI J., NOACK E. J. Cardiovasc. Pharmacol. 14, 13, 1989.
• 12.GRYGLEWSKI R.J., BOTTING R.M., VANE J.R. Hyperten sion 12, 530, 1988.
• 13.GRYGLEWSKI R.J. Tromb. Haemorrh. Disorders 1/2, 1, 1990.
• 14.DRAPIER J.C. Nitric oxide: brain and immune systems. Ed. MONCADA S., NISTICO G., HIGGS E.A. (red.). Portland Press, London & Chapel Hill, pp 205-212, 1993.
• 15.KONOREV E.A., TARPEY M.M., JOSEPH J., BAKER J.E., KALYANARAMAN B. Free Radic. Biol. Med. 18, 169, 1995.
• 16.BECKMAN J.S., BECKMAN T.W., CHEN J., MARSHALL P.A., FREEMAN B.A. Proc. Natl. Acad. Sci. USA 87, 1620, 1990.
• 17.KROCHMAL D., KALINA A. Environ. Pollution 96, 401, 1997.
• 18.KROCHMAL D., KALINA A. Atmospheric Environ. 31, 3473, 1997.
• 19.KROCHMAL D., GORSKI L. Fresenius J. Anal. Chem. 340, 220, 1991.
• 20.KROCHMAL D. International Conference "EURO-ECO 97", Krakow, AGH, pp.55-59, 1997.
• 21.BROWN R.H. Pure Appl. Chem. 65, 1859, 1993.
• 22.WANNER H.U. Experientia 49, 754, 1993.
• 23.YANG S.C., YANG S.P. In: Health and Toxicology. Ed. Gulf Publishing Company, Houston, USA, pp.47-52, 1997.
• 24.BYLIN O., HEDENSTIERNA G., LINDVALL T., SUNDIN B. Eur. Resp. J. 1, 606, 1988.
• 25.KLEEBERGER S.R., LIU-YI Z., JAKOB G.J. Inhal. Toxicol. 9,601, 1997.
• 26.FARMAN C.A., PINKERTON K.E., WITSCHI H., LAST J.A. Inhal. Toxicol. 9, 647, 1997.
• 27.STUDNICKA M., HACKLE., PISCHINGER J., FANGMEY­ER C, HASCHKE N., KUHR J., URBANEK R., NEUMANN M., FRISCHER T. Eur. Resp. J. 10, 2275, 1997.
• 28.FUJIKAWA S., YANG L., WAFFARN F., LERNER M. J. Am. Acad. Audiol. 8, 263, 1997.