Determination of nicotine levels in the leaves of some Nicotiana tabacum varieties cultivated in Syria

• Autorzy: Tayoub G. , Sulaiman H. , Alorfi M.

Warianty tytułu

PL

Oznaczenie zawartości nikotyny w liściach niektórych odmian Nicotiana tabacum urorawianych w Syrii

• Języki publikacji: EN

Abstrakty

EN

Tobacco is the most widely grown non-food crop in the world. Nicotine is the most abundant volatile alkaloid in tobacco leaves. This work aimed at measuring nicotine levels in the leaves of seven different varieties of Nicotiana tabacum, namely: Virginia, Burlip, Katrina, Shk al-bent, Zegrin, Basma and Burley, cultivated in Syria. Nicotine was extracted according to approved method and its concentration was determined by LC/MS/MS in comparison with a standard material dilution series. The percentage of nicotine concentration was calculated manually. Statistical analysis was used to assess the significance of differences among variables and to perform multiple comparisons. The amount of nicotine in dry weight of tobacco leaves represented 6.7% in Virginia variety, 4.9% in Burlip, 4.84% in Katrina, 4.67% in Shk al-bent, 4% in Zegrin, 3.3% in Basma and <3% in Burley. Significant differences in nicotine concentration were found among the different varieties as determined by LSD test at a level of 0.05. This study shows the importance of tobacco varieties grown in Syria, particularly Virginia, Burlip, Katrina, as a cheap and wealthy source for nicotine to be used in some industries.

PL

Wstęp: Tytoń jest najczęściej uprawianą niejadalną rośliną na świecie. Głównym lotnym alkaloidem występującym w liściach tytoniu jest nikotyna. Cel: Celem pracy było oznaczenie zawartości nikotyny w siedmiu różnych odmianach Nicotiana tabacum uprawianych w Syrii: Virginia, Burlip, Katrina, Shk al-bent, Zegrin, Basma i Burley. Metody: Ekstrakcję nikotyny prowadzono według obowiązująch metod, a jej stężenie określono za pomocą LC/MS/MS w odniesieniu do wzorca. Procentowe stężenie nikotyny zostało obliczone ręcznie. Analizy statystycznej użyto do określenia istotności różnic zmiennych i dla porównań wielokrotnych. Wyniki: Zawartość nikotyny w suchej masie liści tytoniu wynosiła 6.7% w odmianie Virginia, 4.9% w Burlip, 4.84% w Katrina, 4.67% w Shk al-bent, 4% w Zegrin, 3.3% w Basma i <3% w Burley. Istotne różnice w stężeniu nikotyny u różnych odmian zostały określone za pomocą testu LSD na poziomie 0,05. Wnioski: Otrzymane wyniki wskazują, że odmiany tytoniu uprawiane w Syrii, zwłaszcza Virginia, Burlip i Katrina mogą być tanim i bogatym źródłem nikotyny stosowanej w różnych gałęziach przemysłu.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2015
• Tom: 61
• Numer: 4
• Opis fizyczny: p.23-30,fig.,ref.

Twórcy

autor

Tayoub G.

• Department of Molecular Biology and Biotechnology, AECS, Damascus, PO Box 6091, Syria

autor

Sulaiman H.

• Department of Molecular Biology and Biotechnology, AECS, Damascus, PO Box 6091, Syria

autor

Alorfi M.

• Department of Molecular Biology and Biotechnology, AECS, Damascus, PO Box 6091, Syria

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Identyfikatory

DOI

10.1515/hepo-2015-0028