Oxidative stress induced by cadmium in Nicotiana tabacum L.: effects on growth parameters, oxidative damage and antioxidant responses in different plant parts

• Autorzy: Martins L.L. , Mourato M. P. , Cardoso A. I. , Pinto A. P. , Mota A. M. , Lurdes S Goncalves M. , Varennes A.
• Języki publikacji: EN

Abstrakty

EN

Tobacco (Nicotiana tabacum L.) is a tolerant species that accumulates cadmium. We studied the effect of Cd (0, 10, 25, 50, 100 µM) on growth parameters, chlorophyll and proline contents, enzymatic antioxidative response and lipid peroxidation of tobacco plants grown in hydroponic culture for 11 days to clarify the strategy of plant response against oxidative stress caused by this heavy metal. Cadmium accumulated more in roots than in shoots. Plant growth was not significantly affected by the cadmium concentrations used. Young leaves were more affected, showing visible chlorosis and a significant decrease in chlorophyll content at high Cd concentrations. Dry weight of both leaves and roots increased indicating a lower capacity for roots to absorb water. An increase in malondialdehyde levels was observed, indicating that lipid peroxidation occurred as a result of ROS formation. The activity of guaiacol peroxidase in leaves increased, indicating that it was very important in the scavenging of H₂O₂, while superoxide dismutase activity only increased in old leaves. Ascorbate peroxidase showed constant activity levels in tobacco leaves, suggesting that the ascorbate–glutathione pathway was less important as a defense mechanism.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 4
• Opis fizyczny: p.1375-1383,fig.,ref.

Twórcy

autor

Martins L.L.

• Departamento de Química Agrícola e Ambiental, UIQA-Instituto Superior de Agronomia, Technical University of Lisbon, 1349-017, Tapada da Ajuda, Lisboa, Portugal

autor

Mourato M. P.

• Departamento de Química Agrícola e Ambiental, UIQA-Instituto Superior de Agronomia, Technical University of Lisbon, 1349-017, Tapada da Ajuda, Lisboa, Portugal

autor

Cardoso A. I.

• Departamento de Química Agrícola e Ambiental, UIQA-Instituto Superior de Agronomia, Technical University of Lisbon, 1349-017, Tapada da Ajuda, Lisboa, Portugal

autor

Pinto A. P.

• ICAAM- Instituto de Ciencias Agrarias e Ambientais Mediterranicas, University of Evora, Herdade Experimental da Mitra, 7000-676, Evora, Portugal

autor

Mota A. M.

• Departamento de Engenharia Quimica e Biologica, Instituto Superior Tecnico, Technical University of Lisbon, Av. Rovisco Pais, 1049-001, Lisbon, Portugal

autor

Lurdes S Goncalves M.

• Departamento de Engenharia Quimica e Biologica, Instituto Superior Tecnico, Technical University of Lisbon, Av. Rovisco Pais, 1049-001, Lisbon, Portugal

autor

Varennes A.

• CEER-Instituto Superior de Agronomia, Technical University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal

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