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The experiment was carried out on 24 turkeys fed for 16 weeks with standard feed diets supplemented with oxidised fat with peroxide values below 5, 50, 100 and 150 meq Cb/kg of fat in groups I, II, III and IV, respetively. The fat (rapeseed oil and poultry fat in 2:1 ratio) was oxidised in controlled conditions and supplemented to feed diets in amounts from 2 to 5% in the subsequent, 4-week, turkey fattening periods. Each group was then divided into two subgroups. One of them wasted with a mix supplemented with antioxidant Hadox dry preparation (0.125 g/kg of fat) containing butylhydroxyanisole and ethoxyquine. Bird slaughter analysis was completed and breast muscles were sampled for meat quality analysis. In the breast muscles the following parameters were determined: pH value, basic chemical composition, the concentration of malondialdehyde (MDA) and in the muscle fat the fatty acid composition was analysed. The muscles underwent sensory evaluation with the use of the flavour profile method immediately after treatment in convection and steam furnace. The application of oxidised fat in the turkey feed mixture significantly decreased the bird body weight without degrading the carcass quality (except the increase in the amount of paraintestinal fat). The presence of the oxidised fat in the feed had an insignificant effect on the concentration of the unsaturated fatty acids in the breast muscle fat, however, it resulted in the increase in MDA and undesirable meat flavour profile changes. Additionally, supplementing the feed with Hadox dry antioxidant did not influence the results of turkey fattening and carcass quality, however, it decreased the concentration of MDA in the analysed muscles and reduced the intensity of the undesirable sensory properties.
The objective of the research study was to apply near infrared (NIR) spectroscopy to evaluate the quality of protein supplements available in the Polish shops and gyms. The evaluation was performed on the basis of the determination of the protein quantity contained in the individual samples by a Kjeldahl method and then the evaluation results were correlated with the measured NIR spectra using an appropriate chemometric method. The research material consisted of fifteen protein supplement samples for athletes, which included the following types: WPI (protein isolate), WPC (protein concentrate), WPH (protein hydrolysate), and mixtures thereof. The obtained NIR spectra of protein supplements were characterized by a similar shape of the bands. Depending on the type of protein, a different intensity of absorption of individual bands could be observed. A Principal Component Analysis (PCA) was used to distinguish the samples based on the spectra measured. Unfortunately, owing to the varying composition of the protein mixtures, it was not possible to find characteristic arrangement of the samples depending on their types. The spectra were correlated with the protein contents determined in the samples using a Partial Least Squares regression method (PLS regression) and various mathematic transformations of the NIR spectral data. The obtained regression models were analysed and the analysis results confirmed that it was possible to apply NIR spectra to estimate the content of proteins in protein supplements. The best result was obtained in a spectrum region between 9401 and 5448 cm⁻¹ and after the first derivative was applied with Multiplicate Scatter Correction (MSC) as a mathematical pre-treatment. On the basis of the results obtained, it was proved that the NIR spectra applied together with the chemometric analysis could be used to quickly evaluate the products studied.
The effect of a diet supplemented with copper (50 ppm) on the uptake and distribution of a single trace dose of ²⁰³HgCl₂ in rats was investigated. The copper-supplemented diet depressed the carcass retention and organ content of mercury-203 given intragastrically. Alterations were accompanied with disturbances in the organ mercury-203 contents. Moreover, the supplements of copper also affected the elimination of an intraperitoneal dose of mercury-203 from the rat body. It is suggested that the alterations in the kinetics of mercury-203 distribution probably resulted from interaction between copper and mercury at the absorption site and from the interaction in the organs. The supplement of copper effectively increases body weight gains and reveals no effect on organ/body ratios and copper contents in organs.
The aim of the study was (1) to determine changes in the total (TC), free (FC) and esterified (EC) L-carnitine (C) in plasma and urine of biathlonists ingesting various doses of C and (2) to find out whether the quantities of C ingested with the diet covered the metabolic needs for this compound. Male (n = 24) and female (n = 22) athletes, aged 17 years were divided in 4 groups given 0, 250, 500 and 750 mg-d"1 of C, respectively. The contents of energy, selected nutrients and of C were assessed by using the food composition tables. Relative C deficiency was determined from FC/TC and EC/FC ratios obtained from plasma measurements. The supplementation with C increased its concentration in plasma and excretion of FC and TC with urine, the levels of EC remaining relatively stable. Mean values of FC/TC and EC/FC ratios were within normal limits and did not depend on the degree of supplementation with C. However, relative C deficiency was observed in two men and five women and could have resulted from a too low C intake with diet or from an intensified catabolism of fatty acids. Supplementing athletes with small doses of C (250-500 mg·d-1) may thus be recommendable.
Male Wistar rats on a standard or vitamin C or selenium supplemented diet received orally a solution of cadmium chloride at a dose corresponding to 10 mg of cadmium/kg of diet. Rats were killed within 28 days of cadmium administration. Results showed that the cadmium intoxication decreased renal and hepatic superoxide dismutase (SOD) activity on day 7 and increased on days 14, 21 and 28. Rats fed the selenium or vitamin C supplemented diet exhibited time- and organ-dependent alterations in SOD activity. In several time-points results varied from those found in intact rats and those treated with cadmium.
The response of broiler chickens on supplemented diets with Avilamycin (10 mg kg-1) or mannanoligosaccharides 1.0 or 2.0 / 1.0 / 0.5 g kg-1 analysed on basic of performance, carcass quality, number of microorganisms in jejunum and caecum content, was determined. The performance results by antibiotic and mannanoligosaccharides (MOS) supplementation of diets were comparable. Reduced number of intestinal pathogenic microflora (E. coli, Clostridium perfringens) was visible (p<0.05) in chickens from MOS (2.0 / 1.0 / 0.5 g kg-1) group in comparisons to control animals. The highest number of Lactobacillus spp. in intestine content was observed in antibiotic group.
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