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The objective of this experiment was to test the possibilities of measuring the rate of DNA synthesis in chicken embryos by applying a simple 15N tracer technique. We hypothesized that the rate of 15N incorporation into liver DNA depends on the type of labelled substance, reflecting precursor availability to provide substrates for nucleotide synthesis. Fertilized eggs were divided into 4 groups (4 × 15): control – not treated, and treated with 15N labeled glycine, ammonium chloride, or sodium nitrate. 15N labeled solutions were given in ovo by injection into albumen. After 20 days of incubation, the labeled substances had no effect on embryo development or morphology. Hepatic DNA was purified and 15N abundance was measured by isotope ratio mass spectrometry. There was significant enrichment of 15N in DNA from the glycine and ammonium chloride groups. We conclude that this simple technique of injecting 15N tracers into incubating eggs can be used to estimate the rate of DNA synthesis.
Twenty-five surficial sediment samples, collected on board ORV Sagar Kanya during her 199th and 200th cruises along a north-south transect between latitudes 9.69◦N and 55.01◦S, and longitudes 80◦E and 40◦ E were studied for isotopic variations (values of δ18O and δ13C) of the indicator planktonic species Globigerina bulloides. The results indicate that from latitudes 9.69◦N to 15◦ S both these isotopes (δ18O and δ13C) fluctuated significantly. Between latitudes from around 15◦S to 30–35◦S δ18O values steadily increased, whereas δ13C showed a decreasing trend. However, to the south of latitudes 30–35◦S, both isotope values showed a similar response with a gradual increase up to latitude 50◦S, beyond which δ18O continued to increase while δ13C declined. The characteristic patterns of the values of both isotopes indicates that the signatures of different water masses are associated with various frontal systems and/or water masses across the transect. The signature of the Polar Front at around latitude 50◦S shows the specific response of the isotopic values (δ18O and δ13C) of G. bulloides. Such a response beyond 50◦S latitude is ascribable to the general decrease in the ambient temperature, resulting in a continuous increase in δ18O values, while δ13C values decrease as a result of reduced photosynthesis in regions approaching higher latitudes owing to low light penetration. To further corroborate our results, those of many such transects from geographically distinct regions need to be studied for isotopic variations in the calcareous shells of planktonic foraminiferal species. The results have the potential to be used as a proxy to assess the movement of frontal systems in southern high latitude regions.
Ecological research using stable isotopes has progressed rapidly during the last 20 years and although more studies are including the addition of isotopically labelled compounds at tracer levels, the overwhelming majority rely on measurements of natural abundance ratios. Access to isotope ratio mass spectrometry has increased, spurred on by awareness of the techniques and increasing demand, and consequently cost of sample analysis has dropped. Today stable isotopes of carbon (¹³C/¹²C), nitrogen (¹⁵N/¹⁴N), sulphur (³⁴S/³²S), oxygen (¹⁸O/¹⁶O), and hydrogen (²H/¹H) can be determined routinely. Perhaps one of the most appealing attributes of isotopic signatures is their potential use to find patterns and determine mechanisms across a range of scales from the molecular level through to characterising whole food webs, reconstructing palaeoenvironments, tracing nutrient fluxes between ecosystems and identifying subsidies, or migrations of organisms. Ecologists from every discipline who are unlikely to have been trained as isotope chemists have added stable isotope analysis (SIA) to their “toolbox”, but often increasing use leads to increasing abuse. The usefulness of SIA arises from predictable physical and enzymatic-based discrimination between biological and non-biological materials leading to different isotopic compositions. Without some ecological understanding of these, interpretation of isotope-derived data can often be flawed. Here, I explore how SIA recently has been used for research in aquatic ecology, reviewing how some of these techniques have allowed elucidation of key processes in aquatic systems such as the contribution of allochthony to lake food webs, and discuss the “state of the art”. Included are some thoughts on where our knowledge in aquatic ecology remains deficient and how continued development and future application of SIA and interdisciplinary methodologies may be applied.
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