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1
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Expression of macrophage surface markers in induced sputum of patients with chronic obstructive pulmonary disease

100%
Domagala-Kulawik J., Maskey-Warzechowska M., Hermanowicz-Salamon J., Chazan R.
Journal of Physiology and Pharmacology. Supplement
|
2006
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tom 57
|
nr 4
75-84
The aim of our study was to evaluate cellular content in induced sputum in chronic obstructive pulmonary disease (COPD) in relation to the degree of airway obstruction, macrophage count, and phenotype. We compared the proportion of macrophages and cells expressing the following markers: CD11b, CD14, CD54, and CD71 in induced sputum obtained from patients with mild-to-moderate and severe COPD (n=29)], asymptomatic smokers (n=18), and nonsmokers (n=18). The differential cell count and macrophage phenotypes were examined in induced sputum by immunocytochemistry. We observed a greater proportion of neutrophils and eosinophils and an elevated macrophage count in patients with COPD and in smokers in comparison with nonsmokers. Macrophages in patients with severe airway obstruction were characterized by a significantly elevated expression of CD11b and CD14 markers. There were higher proportions of macrophages with expression of CD11b, CD14, CD54, and CD71 in induced sputum of smokers in comparison with nonsmokers. We concluded that macrophages are the cells involved in the inflammatory process caused by smoking in COPD. The macrophage phenotype with elevated CD11b and CD14 expressions was associated with severe airflow limitation.
2

Plasminogen activator inhibitor-1 [PAI-1] and urokinase plasminogen activator [uPA] in sputum of allergic asthma patients

100%
Kowal K., Zukowski S., Moniuszko M., Bodzenta-Lukaszyk A.
Folia Histochemica et Cytobiologica
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2008
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tom 46
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nr 2
193-198
3

Biomarkers guided treatment strategies in adult patients with asthma: ready for the clinical field?

100%
Tsilogianni Z., Ntontsi P., Papaioannou A. I., Bakakos P., Loukides S.
Archivum Immunologiae et Therapiae Experimentalis
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2017
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tom 65
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nr 1
4

Nitric oxide production by pulmonary leucocytes from induced sputum in patients with asthma and its effect on epithelial cell viability

100%
Bienkowska-Haba M., Liebhart J., Cembrzynska-Nowak M.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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tom 54
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nr 3
5
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Cathelicidin LL-37, granzymes, TGF-beta1 and cytokines levels in induced sputum from farmers with and without COPD

80%
Golec M., Reichel C., Mackiewicz B., Skorska C., Curzytek K., Lemieszek M., Dutkiewicz J., Gora A., Ziesche R., Boltuc J., Sodolska K., Milanowski J., Spiewak R.
Annals of Agricultural and Environmental Medicine
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2009
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tom 16
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nr 2
289-297
6
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Induced sputum eosinophils, bronchial reactivity, and cough sensitivity in subjects with allergic rhinitis

80%
Tatar M., Petriskova J., Zucha J., Pecova R., Hutka Z., Raffajova J., Brozmanova M.
Journal of Physiology and Pharmacology. Supplement
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2005
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tom 56
|
nr 4
227-236
Some patients with allergic rhinitis and no clinical evidence of asthma exhibit bronchial hyperresponsiveness. In the present study, induced sputum and acetylcholine and capsaicin challenges were assessed in four groups of adult subjects: allergic rhinitis (AR), allergic rhinitis with lower airway symptoms (ARLA), mild stable asthma (BA), and healthy volunteers (C) to correlate lower airway inflammatory markers with bronchial and cough reactivity. Patients with AR (n = 13) and ARLA (n = 11) did not take any anti-inflammatory drugs. Those with BA (n = 9) used inhaled corticosteroids and C (n = 10) were respiratory symptoms free. The patients underwent capsaicin cough challenge and sputum induction with hypertonic saline during the first visit, and acetylcholine bronchial challenge on a separate day. We found that the percentage of eosinophils in induced sputum was significantly higher in patients with AR, ARLA, and BA than in C 14.5 ±1.8(SE) vs. 13.5 ±2.9 vs. 13.9 ±4.0 vs. 3.6 ±0.8 %, respectively (P=0.012). In contrast, acetylcholine PD20 in patients with AR, ARLA, and BA was significantly lower than in C 5.6 ±0.9 vs. 4.1 ±0.4 vs. 2.8 ±0.4 vs. 12.9 ±2.7 mg, respectively (P=0.0001). Neither the eosinophil percentage nor PD20, nor cough sensitivity appreciably differed across the patients groups. Sputum eosinophils correlated significantly with the acetylcholine PD20 (r=0.37, P=0.016). We conclude that eosinophilic inflammation of lower airways and increased bronchial reactivity were present in adult patients with allergic rhinitis.
7
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Sputum screening for lung cancer in radon exposed uranium miners: a comparison of semi-automated sputum cytometry and conventional cytology

80%
Marek W., Richartz G., Philippou S., Marek L., Kotschy-Lang N.
Journal of Physiology and Pharmacology. Supplement
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2007
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tom 58
|
nr 5
8
Content available remote

Comparison of induced sputum and bronchoalveolar lavage fluid cell profile during the treatment of pulmonary sarcoidosis

80%
Mroz R. M., Korniluk M., Stasiak-Barmuta A., Chyczewska E.
Journal of Physiology and Pharmacology. Supplement
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2007
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tom 58
|
nr 5
9
Content available remote

Correlation of exhaled nitric oxide with nitrogen oxides and selected cytokines in induced sputum of chronic obstructive pulmonary disease patients

80%
Ziora D., Dworniczak S., Kaczmarczyk G., Jastrzebski D., Krzywiecki A., Kozielski J.
Journal of Physiology and Pharmacology. Supplement
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2007
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tom 58
|
nr 5
10

Concentrations of plasminogen activator inhibitor-1 (PAI-1) and urokinase plasminogen activator (uPA) in induced sputum of asthma patients after allergen challenge

71%
Kowal K., Moniuszko M., Zukowski S., Bodzenta-Lukaszyk A.
Folia Histochemica et Cytobiologica
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2010
|
tom 48
|
nr 4
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