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Microbes remain active and play an important role in soil nitrogen (N) cycle during the winter in soil of the alpine zone. A shift from microbial N immobilization process dominant during summer to prevailing microbial mineralization process during the winter is observed. Warmer soil under deep snow cover may increase the microbial activity and rate of organic matter decomposition over the winter. Furthermore, severe shortages of dissolved carbon (C) in the winter may cause microbial mortality and lyses. Thus, C limitation on microbial growth and activity may have an important effect on winter N mineralization and even on soil N pools. However, the combined effects of additional organic C (litter inputs) and snow cover on soil N biogeochemical processes in the Tibetan Plateau remain unclear. In the current study, the in situ effects of snowpack and litter decomposition on N dynamics in the alpine zone of the Eastern Tibetan Plateau were investigated. Intact soil core incubations in three different snow regimes (0, 30 and 100 cm depth snow) in the winter were used to solve the problem by measure concentrations of mineral form of soil N. In addition, the litter bag method was used to analyze the litter decomposition over the winter. Our results indicate that the snow cover reduced the ammonium (NH₄⁺-N) content, accelerate N mineralization in soil, and did not significantly change the dissolvable organic nitrogen (DON) and microbial biomass nitrogen (MBN). Meanwhile, snowpack increased the litter N content and accelerated litter decomposition in late winter. Litter addition reduced the MBN and NH₄⁺ -N contents in soil, but increased the nitrate (NO₃⁻ -N) content and net N mineralization, suggesting that N availability to plants during the spring thaw period may be enhanced.
Climate change scenarios suggest that plants will be exposed to increasing levels of ultraviolet radiation in the future. The dove tree, Davidia involucrata Bill (Davidiaceae) is a rare and endangered tree species in China. Its distribution is predicted to become more montane as the climate warms, exposing it to higher levels of UV-B and different soil conditions. We compared the effects of glasshouse ambient (7.08 uW cm–2) and increased (13.93 uW cm–2) UV-B on survivorship, secondary metabolites indicative of radiation damage, and putative defensive responses of Davidia involucrata saplings growing under current and increased nitrogen conditions (5 and 25 g m–2 a–1 N). Mortalities were higher among plants exposed to increased UV-B, but only in soils with high supplementary nitrogen. Increases in compounds associated with defense against radiation were more frequently recorded under high supplementary nitrogen conditions. This rare and localised plant is highly sensitive to elevated UV-B when growing in high nitrogen soils, a combination that is likely to increase in the summer.
Gallery forests in Central Black Sea Region are dominated by Platanus orientalis L. The studies were performed in four sites (Mert River, Adalar, Kurupelit and Taflan Regions) located in V-shaped river valleys and differing with soil conditions. Nutrient concentrations were measured in green and senescent leaves in selected sympatric tree species. Foliar nutrient resorption efficiency (RE; as the ratio of the resorbed amounts of nutrient losses during the leaf senescence to its prior amount deposited in the leaves) and proficiency (RP; the level to which nutrient content per unit leaf mass, mg g⁻¹, has been reduced in senescent leaves) were examined in several sympatric species. The high nitrogen resorption efficiency (N-RE) (62%) were found in Hedera helix L., while the lowest (40%) – in Salix alba L. The phosphorus resorption efficiency (P-RE) ranged between 35% (Rubus discolor Weithe and Nees) and 50% (S. alba) and that of potassium (K-RE) ranged between 49% (S. alba) and 62% (Robinia pseudoacacia L.). The different trend was observed in the values of proficiency (RP). The high nitrogen resorption proficiency (N-RP) ranged between 10 mg g⁻¹ (Clematis vitalba L.) and 18 mg g⁻¹ (R. discolor). The high phosphorus resorption proficiency (P-RP) was found in R. discolor (0.5 mg g⁻¹) similar to N-RP. The lowest P-RP was found in R. pseudoacacia (0.3 mg g⁻¹) like K-RP (5 mg g⁻¹). The high K-RP (10 mg g⁻¹) was found in H. helix. There is no significant difference between four study sites. Soil N, P and organic matter concentrations were significantly correlated with green-leaf N, P and K concentrations. Significant correlations were also found between K-RP and soil nutrient concentrations and soil moisture. However, no significant correlations were found among green-leaf nutrient concentrations, RE, RP and soil nutrient concentrations and moisture.
The experiment was carried out in Slovenia in 2002-03 and 2004-05. The experimental design was a randomized completed block, with four replicates. In the summer after pea harvesting, 10, 20 and 30 kg of Italian ryegrass ha-1 were drilled into minimally cultivated pea stubble, in which, on average, 12.5 pea grains m-2 were left among crop residues. The ryegrass/pea mixtures in our experiments took up the soil mineral nitrogen the most efficiently and accumulated the highest amount of nitrogen in the autumn herb­age yield, at a seeding rate of 30 kg drilled Italian ryegrass. The differences among treatments regarding 0-60 cm soil N03-N and NH4-N contents were highest at the first cut and decreased until March of the next year. In order to achieve efficient early and high N uptake of mineralized nitrogen, it is not recommended to lower the sowing rates of Italian ryegrass for drilling into pea stubble to reduce the high cost of catch crop seeds.
The objective of the study was the estimation of quality of organic matter of the humus horizon of soil fertilised every year, for ten years, with spent mushroom (Agaricus L.) substrate, as compared to a soil without such amendment. Fractionation of organic matter was performed, as well as quality analyses of humic acids and fractionation of organic nitrogen complexes. In the humus horizon of the soil fertilised with spent mushroom substrate lower acidity was noted, and higher levels of C, N, P and Ca. In the soil amended with the substrate greater accumulation of soil humus was noted (by nearly 50%), and especially of the fraction of humic acids, which had a positive impact on increasing the value of the ratio of humic to fulvic acids. The fertilisation with the substrate only slightly modified the quality parameters of humic acids. In the soil fertilised with the substrate a higher level of carbon and nitrogen was observed – related with the soluble forms of organic matter, as well as greater accumulation of nitrogen, mainly in a stable non-hydrolysing form.
ImmunogenW latach 1997-2006, na terenie Lubelszczyzny, przeprowadzono badania środowiskowe dotyczące wielkości uzyskanego plonu ziarna owsa zwyczajnego. Analizowano plonowanie 13 odmian tego gatunku w zależności od stosowanej agrotechniki oraz czynników glebowych. Wielkość plonu ziarna, przedplon, dawkę azotu oraz odmianę ustalono na podstawie wywiadów prze-prowadzonych z rolnikami. Analizę właściwości fizyczno-chemicznych gleby wykonano w akredytowanym laboratorium Okręgowej Stacji Chemiczno-Rolniczej w Lublinie. W próbach glebowych pobranych z warstwy 0-20 cm oznaczono: pH w 1 mol KCl dm-3, skład granulometryczny metodą Cassagrande,a w modyfikacji Prószyńskiego, zawartość C org. metodą Tiurina, zawartość przyswajalnego fosforu i potasu metodą Egnera-Riehma, zawartość przyswajalnego magnezu metodą Schacht-schabela, zawartość N-NH4+ i N-NO3- metodą kolorymetrii przepływowej. Do obliczenia równania regresji wielokrotnej wykorzystano moduł programu Statistica 6 – regresja wielokrotna krokowa po-stępująca, przy F do wprowadzenia 1. Analizę czynnikową (one way ANOVA z półprzedziałami ufności Tukey’a α = 0,05) przeprowadzono po uprzednim pogrupowaniu dawek azotu na sześć klas: N – bez nawożenia, I – 1-25; II – 26-40; III – 41-55; IV – 56-70; V – powyżej 71 kg N ha-1. Stwierdzono, że spośród rozpatrywanych właściwości gleby i zabiegów agrotechnicznych nawożenie azotem w istotny sposób wpływało na plon ziarna owsa, przy czym najwyższy stwierdzono przy dawce powy-żej 71 kg N·ha-1. Uzyskany plon zależał również, w sposób udowodniony statystycznie, od uprawianej odmiany. Współczynniki korelacji wskazują na dodatnią zależność między plonem ziarna owsa a nawożeniem azotem, zawartością mineralnych form azotu w glebie, części ilastych, pH gleby oraz zawartością węgla organicznego. Czynniki te w 33% determinowały zebrany plon.
The application of large amounts of organic or mineral nitrogen (N) fertilizers to soil may cause high risk of nitrogen (N) losses to air and groundwater. A field experiment on regularly cut (5-6 times per year) grassland located on sandy soil was established to evaluate the effect of different fertilization regimes (control without fertilizer, calcium ammonium nitrate (CAN 100), CAN 50/50 in a split dose, ammonium sulphate (SACAM), cattle slurry in 3 doses (SLUR 20/20/20) of 20 tons of cattle slurry in 2 doses (SLUR 30/30) of 30 tons of manure with additional CAN to equal amounts of N added with mineral fertilizer only) on nitrogen losses via N20 production and emission and N03- leaching to deeper soil layers. For better evaluation of used practices (various N fertilization regimes) nitrogen balances for fields have been estimated. Highest fluxes of N20 were found on fields where slurry was applied. Application of slurry in three doses instead of two decreased emissions. Peak fluxes were found two weeks after application (145 μg m-2 h -1 N), and remained so for at least one month. A second fertilization caused another higher peak on the same fields (205 μg-m2-h-1 N). Mineral fertilization caused lower N20 emissions than slurry: Calcium ammonium nitrate used in one dose caused highest fluxes for mineral fertilizers with a peak also two weeks after application (70 μg m-2 h-1 N). On plots where CAN was divided into two rates, fluxes were lower and similar to plots where ammonium sulphate was applied. Generally, fluxes observed in this experiment were low. This could be attributed to a relatively low mineral N content in soil at the beginning of the growth period. Accumulated losses of N20 during the vegetation period until the first cut were highest for the SLUR 30/30 treatment (slurry 30 m3 at the first and 30 m3 at the third cut) and lowest for the CONTROL treatment.
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