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The aim of the present research was to determine the total selenium content in soil and plants from a microplot experiment with different nitrogen fertilization regimes, and to identify the relationships of the selenium content in soil and plants versus the soil catalase activity. The experiment was conducted in randomized blocks with three replications. The soil and plant samples were collected from a microplot experiment established at the IUNG in Pulawy. The soil was enriched with mineral nitrogen and with nitrogen supplied in slurry, both applied at doses of 100 kg N ha-1. The total selenium content in soil under each of the crop rotation systems was no more than 0.2 mg kg-1. Data from the references imply that the soil was deficient in selenium. The highest amount of selenium was under winter wheat and spring barley with undersown crop in crop rotation A, and in soil under maize crop rotation B. Slurry fertilization significantly stimulated the activity of catalase in soil, as compared with the control and mineral nitrogen fertilization treatments. The highest catalase activity – nearly double the control – was detected in soil under winter wheat in crop rotation A and under spring barley in crop rotation B; winter wheat, regardless of the type of crop rotation, accumulated on average 0.3 mg Se kg-1 d.w. in aerial parts and 0.344 mg Se kg-1 d.w. in roots. The highest amounts of selenium in the investigated parts of plants were reported in the control plots and in the plots with slurry fertilization. Mineral fertilization reduced selenium availability to plants. In both crop rotation systems, the highest bioaccumulation of selenium was noted in winter wheat roots from control plots, while the lowest one was detectetd in aerial parts of plants from the plots with slurry fertilization. Despite the fertilization applied, the selenium content in plant roots was higher than its content in aerial parts. The correlation analysis of the results on selenium concentration in soil and plants as well as the catalase activity of soil identified only a significant dependence between the total selenium content and catalase activity in soil from crop rotation B.
Long-term application of fertilizers can change the chemical composition of the soil environment. Our objective has been to determine total amounts of trace elements (Cu, Mn, Zn, Cd and Pb) in the top layer of soil after 34 years of annual applications of organic and mineral fertilizers. The first experiment was set up in 1972 on lessive soil, while the second one was started in 1973 on brown soil. The same crops were grown in both experiments. Bovine manure and slurry were applied in the first experiment, while the other one involved the application of swine manure and slurry. Slurry was applied at two different doses. One dose of slurry was applied together with manure and mineral fertilization in amounts balanced according to nitrogen. The other dose of slurry was determined so that it brought to soil the same amount of organic carbon as introduced with a manure dose. Manure and slurry were also applied in combination with phosphorus and potassium fertilizers in amounts equal half the content of these components used delivered through exclusive mineral fertilization. The total metal content was assessed using the atomic absorption spectrometry method, with prior mineralization in a 1:1 mixture of nitric and chloric acid. The application of fertilizers over many years increased the content of trace elements. The actual effect varied between the analyzed soil types, depending also on the type of fertilizer and the dose of slurry. The average content of Cu, Cd and Pb was 13.8% higher in lessive than in brown soil, while the amount Mn was lower by as much as 32.7%. Among the doses balanced with respect to nitrogen, the effect of manure caused an increase in the Mn, Zn and Ni content in lessive soil, as well as Cu and Pb in brown soil compared to the application of the slurry dose balanced with manure according toorganic carbon. Mineral fertilizers did not increase the content of the analyzed metals as much as manure, with the exception of Cu and Pb in lessive soil and Ni in brown soil.
The penetration of bovine enterovirus, LCR - 4 strain deep into soil profiles in the field conditions was researched. Three soil types were selected: podzolic soil, black earth and browned black earth. The soils differed considerably in respect to their physical properties and chemical analysis. The best conditions for drainage were observed in podzolic soil, whereas the worst - in browned black earth. Experimental plots were being sprinkled with the 1:1 mixture of bovine enterovirus (at the concen-tration of 1x106.0 TCID50/1 ml) and slurry. The enterovirus researched, in any soil, migrated to the depth of 7.5 to 10 cm. The highest titres were observed in the surface layers of black earth and podzolic soil (0-2.5 cm). They differed from 102.72 - 104.27 TCID50/g of the soil. With depth the titres decreased, with slight fluctuations observed.
Проведен анализ условий, влияющих на интенсификацию процесса метанового сбраживания. Определена степень влияния на эффективность производства биогаза и его тепловую ценность температурного режима метантенка, сырьевой базы, перемешивания, наличия коферментаторов
The subject of this study was an assessment of the effectiveness of hygienization of pig slurry during the methane fermentation process. The basic criterion for evaluating the effectiveness of the process was the inactivation rate of Salmonella Senftenberg (W775, H2S negative) and the eggs of Ascaris suum. Bacteria suspensions were introduced into slurry inside microbiological carriers of the FILTER-SANDWICH type, while parasite eggs were in special perlon parasitological carriers. The obtained results indicate that during anaerobic digestion of slurry there occurred a rapid elimination of both Salmonella Senftenberg (W775, H2S negative) and Ascaris suum eggs. The shape of the regression line shows that the complete inactivation of Salmonella Senftenberg (W775, H2S negative) occurred between the 12th and 13th hour of the process. Ascaris suum eggs were completely eliminated after 4 hours. Study results suggest that even in the case of strong contamination of the feed material, the technology of anaerobic digestion guarantees obtaining a microbiologically safe product.
Mineral and organic fertilization is one of the rtiost important factors affecting activity of soil enzymes. It is commonly accepted that organic fertilization is more beneficial to the soil biological activity than the mineral. The objectives of this investigation were to assay Corg, Ntot, Ptot content and to determine urease and amylase activities in the soil fertilised for a long time with various rates of farmyard manure and slurry. Soil samples were taken from the experiment established on a typical lessivé soil. FYM was used at the following rates: 20, 40, 60, 80 t ha-1 fresh weight once in the rotation on the plots with potatoes, while slurry was applied in four doses corresponding to manure fresh weight. Soil samples were taken after potato harvest in the 8th year after the experiment start-up from two depths: 5-15 and 25-35 cm of the soil profile. Usually, higher amounts of Corg and Ptot were found in the soil manured with FYM in comparison with the pig slurry-manured soil. These amounts increased when the doses of both fertilizers were the highest. The content of Corg ranged from 5.5 to 8.2 g kg-1 of soil when 80 t ha-1 of FYM was used. Total nitrogen ranged from 0.45 to 0.95 g kg-1 of soil, average for doses of both fertilizers, whereas total phosphorus content ranged from 0.26 to 0.48 g kg-1 of soil. The highest amylase activity was observed in the soil when FYM was used (0.48 ng starch g-1 l6 h-1) as compared to the soil where slurry was applied (0.41 g starch ug-1 16 h-1). No increase of amylase activity was observed when the doses of both fertilizers were the highest. A higher urease activity was noted when FYM was used than in the case of the soil manured with slurry.A decrease of urease activity was noted in the control samples (3.0 mg NH3 10 g-1 24 h-1), while the urease activity increased when the doses of both fertilizers were the highest. The highest activities were noted when 80 t ha-1 of FYM or slurry were used.
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