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Combining ability analysis of seven silk yield attributes in silkworm, Bombyx mori L.

100%

Bhargava S. K.

Journal of Applied Genetics

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1995

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tom 36

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nr 1

The combining ability effects of five silkworm (Bombyx mori L.) strains and their 20 F₁'s including reciprocals were analysed in a 5x5 diallel cross for seven silk yield attributes, viz. effective rate of rearing (ERR), a measure of survival, cocoon yield, cocoon weight, shell weight, raw silk percentage, silk filament length/cocoon and silk reelability. Higher values of specific combining ability (SCA) than the corresponding general combining ability (GCA) for all the attributes except ERR and cocoon yield are indicative of non-additive gene action. The parental strain JC2P was the best general combiner for all the attributes, except cocoon yield. The highest general combining ability effect for cocoon yield was shown by N4. The highest desirable or positive SCA effects resulted from N4 × SH2 (all the attributes except shell weight), JC2P × 14M (all the attributes except cocoon weight and shell weight), 14M × SH2 (all the attributes except ERR), N4 × SPJ1 (all the attributes except shell weight and raw silk percentage). Only one reciprocal, namely SPJ1 × N4, showed positive effects for all the attributes except silk reelability. The improvement of cocoon yield through mass selection followed by intermating and the use of the parental strain JC2P in future cross breeding are discussed.

2

The genomic survey of Tc1-like elements in the silkworm microsporidia Nosema bombycis

75%

Song H., Tang X., Lan L., Zhang X., Zhang X.

Acta Parasitologica

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2020

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tom 65

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nr 1

3

The telomere-specific non-LTR retrotransposons SART 1 and TRAS1 are suppressed by PIWI subfamily proteins in the silkworm, Bombyx mori

63%

Tatsuke T., Sakashita K., Masaki Y., Lee J. M., Kawaguchi Y., Kusakabe T.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 1

118-133

The telomere structures in Bombyx mori are thought to be maintained mainly by the transposition of the specialized telomeric retroelements SART and TRAS. The silkworm genome has telomeric TTAGG repeats and telomerase, but this telomerase displays little or no activity. Here, we report that the transcription of the telomeric retroelements SART1 and TRAS1 is suppressed by the silkworm Piwi subfamily proteins BmAgo3 and Siwi. The silkworm Piwi subfamily was found to be expressed predominantly in the gonads and early embryo, as in other model organisms, but in BmN4 cultured cells, these proteins formed granules that were separate from the nuage, which is a different behaviour pattern. The expression of TRAS1 was increased in BmN4 cells when BmAgo3 or Siwi were silenced by RNAi. Our results suggest that B. mori Piwi proteins are involved in regulating the transposition of telomeric retroelements, and that the functional piRNA pathway is conserved in BmN4 cultured cells.

4

Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae

63%

Huang D., Chao Y.-C., Lv Z., Jan J.-T., Yang Y.-C., Hsiao P.-W., Wu C.-Y., Liao C.-H., Wu T.-H., Wang Y.-C.

Journal of Veterinary Research

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2021

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tom 65

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nr 2

5

Genetic diversity among microsporidian isolates from the silkworm, Bombyx mori, as revealed by randomly amplified polymorphic DNA (RAPD) markers

63%

Surendra Nath B., Hassan W., Nageswara Rao S., Vijaya Prakash N. B., Gupta S. K., Madana Mohanan N., Bajpai A. K.

Acta Parasitologica

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2011

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tom 56

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nr 4

Random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was carried out to assess the genetic diversity of five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworms. A type species, NIK-1s_mys was used as control for comparison. Differences in the spore shape, length and width were observed. Of the 30 decamer random primers tested, 22 primers gave repeatable RAPD profiles and yielded a total of 143 fragments, of which 78 were polymorphic (55%). The resulting data was used to derive genetic similarity values for constructing a dendrogram. The neighbour joining method based on Dice coefficients indicate a major cluster comprising NIK-1s_mys, NIWB-11bp and NIWB-12n, whereas NIWB-13md, NIWB-14b and NIWB-15mb appear to be different from each other as well from the major cluster mentioned above which includes the type species (NIK-1s_mys). Based on the reproducibility of RAPD profiles, we are able to identify these microsporidians as different isolates. The RAPD technique may be useful in detecting sources of infection of this economically important domestic insect.

6

Usability of silkworm pupa meal in the feeding of honey bee [Apis mellifera L.]

63%

Szymas B., Soszka M., Przybyl A.

Animal Science. Scientific Papers of Agricultural University of Poznan

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2000

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tom 02

7

Molecular characterization and phylogenetic relationships among microsporidian isolates infecting silkworm, Bombyx mori using small subunit rRNA (SSU-rRNA) gene sequence analysis

51%

Surendra Nath B., Gupta S. K., Bajpai A. K.

Acta Parasitologica

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2012

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tom 57

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nr 4

The life cycle, spore morphology, pathogenicity, tissue specificity, mode of transmission and small subunit rRNA (SSU-rRNA) gene sequence analysis of the five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworm, Bombyx mori have been studied along with type species, NIK-1s_mys. The life cycle of the microsporidians identified exhibited the sequential developmental cycles that are similar to the general developmental cycle of the genus, Nosema. The spores showed considerable variations in their shape, length and width. The pathogenicity observed was dose-dependent and differed from each of the microsporidian isolates; the NIWB-15mb was found to be more virulent than other isolates. All of the microsporidians were found to infect most of the tissues examined and showed gonadal infection and transovarial transmission in the infected silkworms. SSU-rRNA sequence based phylogenetic tree placed NIWB-14b, NIWB-12n and NIWB-11bp in a separate branch along with other Nosema species and Nosema bombycis; while NIWB-15mb and NIWB-13md together formed another cluster along with other Nosema species. NIK-1s_mys revealed a signature sequence similar to standard type species, N. bombycis, indicating that NIK-1s_mys is similar to N. bombycis. Based on phylogenetic relationships, branch length information based on genetic distance and nucleotide differences, we conclude that the microsporidian isolates identified are distinctly different from the other known species and belonging to the genus, Nosema. This SSU-rRNA gene sequence analysis method is found to be more useful approach in detecting different and closely related microsporidians of this economically important domestic insect.

8

FMRFamide-related peptides regulate the prothoracic gland activity

51%

Yamanaka N., Zitnan D., Hua Y.-J., Mizoguchi A., Tanaka Y., Kataoka H.

Pestycydy

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2005

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nr 3

147-152

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Selective toxicity of diafenthiuron to non-target organisms: honey bees, coccinellids, chelonus, earthworms, silkworms and fish

51%

Stanley J., Chandrasekaran S., Preetha G., Kuttalam S., Jasmine R. S.

Journal of Plant Protection Research

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2016

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tom 56

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nr 1

Diafenthiuron, an insecticide widely used in the management of pests of cardamom and cotton, was assessed for its toxicityeffect on beneficials commonly found in these ecosystems. Diafenthiuron was found to be toxic to honey bees, the prime pollinators of crop plants. Diafenthiuron at the highest tested dose caused 40% mortality to the coccinelid grubs at 48 h after treatment so, diafenthiuron was found to be slightly harmful. Monocrotophos, on the other hand has been found to be a highly toxic pesticide. Diafenthiuron is moderately harmful to the adults of Chelonus blackburni L. The testing was done using the insecticide diafenthiuron. An insecticidecoated vial (scintillation) bioassay was performed. It was found that there was 86.67% mortality in 48 h, at the recommended dose. Diafenthiuron is highly toxic to the silkworm, killing more than 80% of the caterpillars in 24 h, at all the doses tested. Diafenthiuron, even in the highest dose tested, is non-toxic to the earthworm, Perionyx excavatus, which was found to have a 3.33% mortality. As far as fish are concerned, the common carp, Cyprinus carpio L., is found to be highly susceptible to diafenthiuron and even doses 10 times lower than the field dose can kill the fish within 6 h.

Ograniczanie wyników

Combining ability analysis of seven silk yield attributes in silkworm, Bombyx mori L.

The genomic survey of Tc1-like elements in the silkworm microsporidia Nosema bombycis

The telomere-specific non-LTR retrotransposons SART 1 and TRAS1 are suppressed by PIWI subfamily proteins in the silkworm, Bombyx mori

Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae

Genetic diversity among microsporidian isolates from the silkworm, Bombyx mori, as revealed by randomly amplified polymorphic DNA (RAPD) markers

Usability of silkworm pupa meal in the feeding of honey bee [Apis mellifera L.]

Molecular characterization and phylogenetic relationships among microsporidian isolates infecting silkworm, Bombyx mori using small subunit rRNA (SSU-rRNA) gene sequence analysis

FMRFamide-related peptides regulate the prothoracic gland activity

Selective toxicity of diafenthiuron to non-target organisms: honey bees, coccinellids, chelonus, earthworms, silkworms and fish