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Importance of explant size and origin for cryopreservation of Rosa pomifera “Karpatia” shoot tips by a droplet vitrification method

100%

Kwasniewska E., Dziedzic E., Pawlowska B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

2

In vitro regeneration of Plantago media L. from shoot tips and other seedling explants

86%

Budzianowska A., Malkiewicz M., Budzianowski J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

3

Cryopreservation of cherry rootstock Gisela 5 (Prunus cerasus × Prunus canescens) shoot tips by droplet-vitrification technique

86%

Ruzic D., Vujovic T., Cerovic R.

Journal of Horticultural Research

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2013

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tom 21

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nr 2

4

Micropropagation of Senecio macrophyllus M.Bieb.

86%

Trejgell A., Michalska M., tretyn A.

Acta Biologica Cracoviensia. Series Botanica

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2010

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tom 52

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nr 1

67-72

This is the first protocol for in vitro micropropagation of Senecio macrophyllus. Shoot tips and fragments of the cotyledon, hypocotyls and roots were isolated from 10-day-old sterile seedlings. The morphological response was tested on MS medium supplemented with different types of cytokinins: BA (2.2 μM, 4.4 μM or 13.3 μM), KN (4.7 μM or 13.9 μM) and ZEA (4.6 μM or 13,7 μM) in combination with 0.54 μM NAA or 0.27 μM NAA (with 2.2 μM BA only), but only shoot tips were capable of shoot organogenesis. Shoot proliferation was highest for explants cultured on MS medium supplemented with 4.4 μM BA in combination with 0.54 μM NAA. The shoots formed were then multiplied on the same medium. Rooting was achieved on full- and half-strength MS medium without auxin, but shoots cultured on medium BA-supplemented began inducing roots a week later than shoots obtained on media with other types of cytokinins. Well-rooted plantlets were transferred to ex vitro conditions. The survival rate of rooted plants was 100% for plants cultured in a mixture of vermiculite and sand, and 92% for those planted in soil after 4 weeks of acclimatization. In the first year the plants grew intensively under field conditions and were able to develop a leaf rosette. In the second year the plants were able to flower and produce viable seeds.

5

A protocol for synthetic seeds from Salvia officinalis L. shoot tips

72%

Grzegorczyk I., Wysokinska H.

Acta Biologica Cracoviensia. Series Botanica

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2011

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tom 53

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nr 1

Shoot tips excised from shoot culture of Salvia officinalis were encapsulated in 2% or 3% (w/v) sodium alginate and exposed to 50 mM calcium chloride for complexation. Immediately or after 6, 12 or 24 weeks of storage at 4°C, the synthetic seeds were cultured for 6 weeks on half-strength MS medium supplemented with indole-3- acetic acid (IAA) (0.1 mg/l) and solidified with 0.7% agar. The frequency of shoot and root emergence from encapsulated shoot tips was affected by the concentrations of sodium alginate and additives in the gel matrix (sucrose, gibberellic acid, MS nutrient medium) as well as duration of storage. The frequency of shoot and root induction of non-stored synthetic seeds was highest with shoot tips encapsulated with 2% sodium alginate containing 1.5% sucrose and 0.5 mg/l gibberellic acid (GA3). Shoot tips maintained their viability and ability to develop shoots even after 24 weeks of storage when they were encapsulated in 3% alginate with 1/3 MS medium, sucrose (1.5%) and GA3 (0.25 mg/l). Root formation tended to decrease with storage time. Overall, 90% of the plantlets derived from stored and non-stored synthetic seeds survived in the greenhouse and grew to phenotypically normal plants. This procedure can enable the use of synthetic seed technology for germplasm conservation of S. officinalis, a plant species of high medical and commercial value.

6

In vitro regeneration of Vitellaria paradoxa from shoot tip explants

72%

Afful N. T., Abdulai I., Azu E., Elegba W., Annor W., Akama C., Asare K., Dentey J., Amoatey H. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2022

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tom 103

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nr 1

7

Micropropagation of cloudberry [Rubus chamaemorus L.] by initiation of axillary shoots

72%

Thiem B.

Acta Societatis Botanicorum Poloniae

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2001

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tom 70

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nr 1

8

In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants

72%

Piatczak E., Wysokinska H.

Acta Societatis Botanicorum Poloniae

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2003

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tom 72

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nr 4

Various explants from 30-day-old seedlings of Centaurium erythraea Rafn were evaluated for their morphogenetic capacity under in vitro culture conditions. Shoot formation from shoot tip explants was achieved mainly through adventitious bud differentiation. The highest number of shoots (up to 43.3 ± 2.2 from a single shoot tip) was obtained on Murashige and Skoog medium (MS) supplemented with indole-3-acetic acid (IAA) (0.57 μM) and 6-benzylaminopurine (BAP) (4.4 μM). Adventitious shoot regeneration was also achieved through organogenesis from calluses obtained from hypocotyls, cotyledons, roots and leaves on MS medium containing IAA (2.85 μM) and BAP (0.88 μM). Significant differences were noted between explant types in their effects on shoot regeneration. In the primary culture, the best response was obtained either from calluses derived from roots or leaves (44.4 ± 4.5 and 40.2 ± 6.0 shoots per callus, respectively). The number of subcultures of inoculated calluses affected both the multiplication rate (the number of shoots/explant) and shoot morphology (the frequency of shoot hyperhydricity). Shoots rooted with the frequency of 94-100% after culture on MS medium without growth regulators. Plantlets were successfully acclimatized (97%) under high relative humidity and then moved to the greenhouse.

9

Effect of various preculture and osmotic dehydration conditions on cryopreservation efficiency and morphogenetic response of chrysanthemum shoot tips

51%

Kulus D., Serocka M., Mikula A.

Acta Scientiarum Polonorum. Hortorum Cultus

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2018

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tom 17

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nr 1

The aim of this study was to evaluate the influence of sucrose concentration in the preculture medium, as well as the duration of osmotic dehydration on the efficiency of chrysanthemum ‘Richmond’, ‘Lady Orange’ and ‘Lady Salmon’ cryopreservation by encapsulation-dehydration technique. For all cultivars tested, the best regrowth of cryopreserved shot tips was recorded with 0.25 M sucrose concentration and 10 µM ABA during a two-week preculture, followed by a 4- or 7-day osmotic dehydration. The survival rate ranged from 56.8–58.0% (the Lady group) to 63.6% (‘Richmond’). However, the ability to grow was smaller and reached 18.2–50.7%. It was found that higher sucrose concentration during the preculture slowed the growth of chrysanthemum shoot tips and led to an increased formation of multiple shoots (by activating axillary buds) or deformed adventitious shoots (incapable of further growth). The frequency of tissue hyperhydricity also increased, while the rhizogenesis efficiency decreased when higher sucrose concentration in the preculture medium was applied. The influence of osmotic dehydration duration on the explants morphogenetic response was cultivar-dependent.

Ograniczanie wyników

Importance of explant size and origin for cryopreservation of Rosa pomifera “Karpatia” shoot tips by a droplet vitrification method

In vitro regeneration of Plantago media L. from shoot tips and other seedling explants

Cryopreservation of cherry rootstock Gisela 5 (Prunus cerasus × Prunus canescens) shoot tips by droplet-vitrification technique

Micropropagation of Senecio macrophyllus M.Bieb.

A protocol for synthetic seeds from Salvia officinalis L. shoot tips

In vitro regeneration of Vitellaria paradoxa from shoot tip explants

Micropropagation of cloudberry [Rubus chamaemorus L.] by initiation of axillary shoots

In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants

Effect of various preculture and osmotic dehydration conditions on cryopreservation efficiency and morphogenetic response of chrysanthemum shoot tips