Studies on food preference of two-spotted spider mite Tetranychus urticae Koch were carried out on 21 perennial weed species in cereals. Feeding of females of two-spotted spider mite placed on leaf disk of selected weed species lasted the longest on Ranunculus regens and Amoracia lapathifolia. Meanwhile, phase of initial feeding of two-spotted spider mite was the shortest. Time spent on searching for food varied, and the longest one was on Helichrysum arenarium, Agrimonia euparoria and Saponaria officinalis, while the shortest on R. regens and A. lapathifolia. However, if females of two-spotted spider mite had for choice 21 perennial weed species, then they preferred most Convolvulus arvensis, Urtica dioica and Ballota nigra, but least H. arenarium, Artemisia absinthium, Artemisia vulgaris, Medicago palcata and Taraxacum officinale.