One of the main purpose of the research on remote sensing in plant protection is to develop simple and cheap method to detect symptoms of diseases and pests of agricultural crops in early stages of development. The aim of this study was to determine the possibility of using an ASD Field Spec3 hyperspectral radiometer to detect and monitor the development of Puccinia recondita f. sp. recondita Roberge on winter rye. The highest values of a coefficient of determination (R2 = 0.84) was obtained for the relationship between degree of infestation of leaves and values of the NDVI1 and ARI indices, which were calculated on the basis of reflectance of green (550 nm) and red (700 nm) wave lengths.