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Background. Pullulan is a water-soluble polysaccharide produced by fungi Aureobasidium pullulans. This glucan was applied for coating of food products. The aim of this study was obtaining of a pullulan coating and checking its effect on growth of microorganisms responsible for spoilage of food. Materials and methods. Pullulan produced by the white mutant A. pullulans B-1 was applied. Permeability of oxygen and carbon dioxide through film produced from 10% water solution of pullulan was checked as well as degree of inhibition of chosen microorganisms through pullulan coating formed on surface of growth‟s media. Results. Low permeability of gases through pullulan film and a considerable growth‟s limitation of all tested microorganisms were found. A total growth‟s inhibition of 21 from 36 tested strains and a partial growth‟s limitation of the remaining 15 strains was observed. The inhibitory effect was diverse and it was from 63 to 100%. Conclusions. These results proved that pullulan coating applied in these tests revealed big barrier characteristics in relation to oxygen and carbon dioxide, which had effect upon growth‟s inhibition of most of the tested microorganisms, responsible for spoilage of food
The aim of the research was to study the influence of the pullulan and pullulan- -protein edible coatings on the reduction of the apples mass loss during the storage. The research was carried out on pullulan received from A. pullulans white mutant B-1 in a batch cultivation process and the ‘Malinova’ and ‘Champion’ apples. In the first stage of the research the apples were covered with 15% and 20% pullulan water solution and storage at 4°C and 22°C during 39 days. In the second stage of the research the apples were covered with pullulan-protein coating obtained from the mixture of pullulan and soy protein. Apples covered with the pullulan-protein coatings were stored at the temperature of 2°C during 10 weeks. Mass losses of apples, durability of the pullulan and pullulan-protein coatings as well as changes in the appearance of the surface coating covered fruit in comparison with the one uncovered were evaluated. Pullulan edible coating significantly limited apples mass losses. Apples covered with coatings showed lower mass losses than the ones uncovered. The smallest mass losses were observed in apples covered with the coatings where the pullulan to protein ratios were: 6:4 and 5:5. It was observed that by adding protein to pullulan the coating stuck better to apples surface. During the storage process the protein-containing layer was less susceptible to crumbling and to peeling off.
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