Wyniki wyszukiwania

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The heterogeneity of cell subtypes from a primary culture of human amniotic fluid

100%

Zhang S., Geng H., Xie H., Wu Q., Ma X., Zhou J., Chen F.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 3

424-439

Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells.

2

Primary culture of cat intestinal epithelial cells in vitro and the cDNA library construction

84%

Zhao G. H., Liu Y., Cheng Y. T., Zhao Q. S., Qiu X., Xu C., Xiao T., Zhu S., Liu G. Z., Yin K.

Acta Parasitologica

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2018

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tom 63

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nr 2

3

Acute cytotoxicity evoked by tetrabromobisphenol A in primary cultures of rat cerebellar granule cells outweighs the effects of polychlorinated biphenyls

84%

Zieminska E., Stafiej A., Toczylowska B., Lazarewicz J. W.

Polish Journal of Environmental Studies

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2012

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tom 21

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nr 4

The aim of this study was to identify, among selected environmental toxins, the substance with the highest in vitro toxicity to neurons combined with the most marked induction of calcium imbalance, oxidative stress and mitochondrial dysfunction. Exposure of primary cultures of rat cerebellar granule cells for 30 min to polychlorinated biphenyls (PCBs) or brominated flame retardants (BFRs) at concentrations of 10-50 µM identified tetrabromobisphenol A as the compound with the highest toxicity. At a concentration of 25 µM, apart from the moderate activation of ⁴⁵Ca uptake, this BFR induced the most pronounced increase in intracellular Ca²⁺ concentration, depolarization of mitochondria, and activation of ROS production.

4

The delay of anoikis due to the inhibition of protein tyrosine dephosphorylation enables the maintenance of normal rat colonocyte primary culture

84%

Dzierzewicz Z., Orchel A., Parfiniewicz B., Weglarz L., Stojko J., Swierczak-Zieba G., Wilczok T.

Folia Histochemica et Cytobiologica

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2003

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tom 41

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nr 4

5

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Physiological response of in vitro cultured Magnolia sp. to nutrient medium composition

67%

Sokolov R. S., Atanassova B. Y., Iakimova E. T.

Journal of Horticultural Research

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2014

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tom 22

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nr 1

6

Oestrogen effects on kainate-induced toxicity in primary cultures of rat cortical neurons

67%

Kajta M., Lason W.

Acta Neurobiologiae Experimentalis

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2000

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tom 60

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