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The investigations of structure of the genets and populations of Iris sibirica L. were carried out in the period 1999–2002. The studies were conducted in Molinietum caeruleae patches localized in Southern Poland. The patch labelled MW (“more wet”) measured 5,600 m² and was chracterised by presence of large depressions filled with stagnant water, concentrated chiefly in the central part of the site. In this locality species with a high competitive potential (like Phagmites australis Trin., Chamanerion angustifolium (L.) Scop., Epilobium hirsutum L., Filipendula ulmaria (L.) Maxim. and others) prevailed. The patch called LW (“less wet”) measured 1,800 m² and was characterised by small number of depressions scattered throughout the site. In this locality dominated the plants of minor competitive abilities (like Lathyrus pratensis L., Lotus corniculatus L. and others). The spatial pattern of populations was described on the basis of the locations of all ramet clusters in 100 m² square plots. Each ramet cluster was attributed to a developmental stage. The differences in height of ramets between stages was estimated on the basis of lenght of the tallest leaf and generative shoot measured in all genets and ramet clusters in both populations (30 leaves and all generative shoots in 5 clusters of ramet at generative and senile stages and 4 – at fragmentation phase in both populations). In study year 2000 Iris sibirica population in LW locality was consisted of 26 ramet clusters and showed an aggregation structure, while population growing in MW site was consisted of 502 ramet clusters and presented the group-aggregation spatial structure constituted by groups of dozen or so ramet clusters, forming large aggregations. Both populations showed signs of senility in effect of absence of genets in prereproductive stages. In 2001 and 2002 the seedlings and juvenile genets appeared only in artificially made gaps. The genets in initial stage are much lower (about 40 %) than older ones and the ramet in MW locality reached greather height (6 to 23%) than in LW site. The evaluation of structure of populations taken as indicator of their vitality, show that the population from less wet locality was in better condition despite of low abundance. Domination of ramet cluster at generative stage and vicinity of plants with low competitive potential enables longer existence of Iris sibirica in occupied site. In more wet locality the I. sibirica population is worse equiped to survive. Advanced senility and the vicinity of plants with high competitive potential might inhibit the proliferation of I. sibirica ramet clusters, accelerate their fragmentation or even eliminate from occupied site.
To analyse the population structure and reproductive biology of the Iberian hare Lepus granatensis Rosenhauer, 1856, 498 hares (264 males and 234 females) were collected in monthly samples from October 1998 to September 1999. Females reached larger sizes than males, with approximately 400 g difference in body mass on reaching sexual maturity. The total sex ratio was 1:1, with a bias in favour of males in winter. Sexually active males and females appeared in every month but August, when no sexually active female was found. Births occurred in every month and were more frequent between March and July. Seasonal variation in kidney fat index (KFI) followed a similar pattern in males and females, with an increase in mid-autumn and a decline at the end of winter. Reproductive activity appeared in every month, with a maximum from February to June. Reproductively hyperactive females (simultaneously pregnant and nursing) appeared in every month except in January, with a first peak in March and a second lower peak in May-June. Litter size fluctuated between 1 and 7 leverets. The most frequent gestations involved 1 or 2 foetuses. The mean annual litter size was 2.08 and the average number of litters per productive female per year was estimated to be 3.48. The maximum productivity was recorded between March and May. The total annual production of young per adult female was estimated to be 7.21. The minimum annual survival rate of young was 27.91%. On the basis of these results we propose to maintain the limit of the hunting period between October and December. We discuss the relationship between the low young/adult ratio obtained and the hunting method used.
Modern technologies and accurate information on genetic diversity and structure are contributing to improve the plant breeding, in particular for all the minor species with a lack of data. Genetic diversity of 139 different Ficus carica L. genotypes collected from Italy and Croatia, and divided into two subgroups: uniferous (only main crop) and biferous (breba and main crop), was investigated using 49 microsatellite markers. A total of 70 alleles were generated, of which 64 (91.4%) showed a polymorphic pattern indicating high level of genetic diversity within the studied collection. The mean heterozygosity over the 64 single locus microsatellites was 0.33 and the expected and observed averaged variance were 16.50 and 184.08, respectively. The 139 fig genotypes formed two clusters in the PCoA analysis, suggesting a division between Italian and Croatian genotypes. Moreover, the fig accessions could be divided into two main clusters based on the STRUCTURE analysis according to the biological type, uniferous or biferous, with partly overlapping varieties. In conclusion, our results demonstrated that molecular markers were able to discriminate among genotypes and useful for the authentication of fig tree varieties (homonymies and synonymies).
Based on previously published electrophoretic data on genetic variability in 31 roe deer Capreolus capreolus Linnaeus, 1758 populations, the proportion of loci polymorphic (P), average heterozygosity (H), and the inbreeding coefficient (FIS) were examined for relationships with the social structure displayed in the various populations. Our hypothesis was that genetic variability is lower and FIS-values are more positive in populations where males maintain a stable pattern of territories during the rutting season (forest dwelling roe deer) than in those characterized by pronounced fluctuations in population structure, both within and among seasons (field or mountain dwelling roe deer). P and H did not show differences among those two groups. FIS was significantly more positive in the 'forest' roe deer than in the more migratory 'type', but only when populations subjected to high culling rates were excluded from the analysis. Highly negative FIS-values in forest populations with high culling rates suggested that considerable perturbations of population structure may be caused by hunting. In conclusion, the 'forest' roe deer and the 'field' roe deer do not represent two distinct ecotypes with a particular genetic integrity, but rather reflect the considerable behavioural plasticity of the species.
The paper presents results of a study conducted in Ińsko Landscape Park in the years 2002 and 2003. The study has confirmed the occurrence of 11 amphibian species, which coincides with results obtained previously by other researchers. The presence of two amphibian species from the “Red List of Threatened Species in Poland”, namely Triturus cristatus and Bombina bombina has been confirmed. Another important result of the study is the confirmation of the occurrence of Bufo calamita in the studied area. Finally, the paper presents the dynamics of occurrence of selected species.
The genetic diversity within and among nine natural populations of Thymus hirtus Willd. subsp. algeriensis (Boiss. et Reut.) Murb. from different geographical and bioclimatic zones were assessed using Random Amplified Polymorphic DNA data. A total of 154 bands were generated from seven selected primers. 141 bands were polymorphic (P=91.56%). The genetic diversity within a population based on Shannon's index which was high (H'pop=0.307) and varied according to bioclimatic zones. A high genetic differentiation among populations (GST=0.335 and FST=0.296) was revealed, suggesting a population isolation and a low level of gene flow among them. The major proportion of the variation was attributable to individual differences within populations. The genetic structure is in accordance with geography distances. The Neighbour- joining tree based on Nei's and Li's genetic distance among individuals showed that individuals from each population clustered together. The UPGMA dendrogram based on FST values revealed three clusters each of them includes populations closuring to their geographical origin. The high genetic structure of populations resulted from genetic drift caused both by the habitat fragmentation and the low size of most populations. The in situ conservation should interest all populations. The ex situ conservation should be based on the collection of seeds rather within than among populations because of the maximum of variation was revealed within populations.
The genetic diversity of two Aconitum species endemic to the Carpathian Mountains and Sudetes was studied. A reticulate evolution between them was earlier postulated as an effect of secondary contact. The genetic diversity at the individual and taxonomic levels was examined across the entire geographical ranges of the taxa in 11 populations based on 247 AFLP markers found in 112 individuals in the Sudetes and Western Carpathians. The overall genetic differentiation was greater within the Sudetic A. plicatum (Fst = 0.139, P < 0.001) than within the Carpathian A. firmum (Fst = 0.062, P < 0.001), presumably due to the long-lasting geographic isolation between the Giant Mts and Praděd (Sudetes) populations of the species. Interestingly, relatively distant and presently isolated populations of A. plicatum and A. f. subsp. maninense share a part of their genomes. It could be an effect of their common evolutionary history, including past and present reticulations. The introgression among infraspecific taxa of Aconitum is common, probably as a result of seed dispersal within a distance of ca. 20 km (Mantel's r = 0.36, P = 0.01). Aconitum f. subsp. maninense had the highest genetic diversity indices: Nei's h and rarefied FAr, and divergence index DW (P < 0.05), pointing to its presumably ancient age and long-term isolation.
Twenty years of the Lowland European bison Bison bonasus bonasus conservation in captivity. The aim of the work was to track back changes taking place in the Lowland European bison population kept in captivity in years 1995-2015. The material for the study was data from European Bison Pedigree Book, collected from the years 1924-2015. The parameters such as inbreeding coefficient, kinship coefficient, contribution of founder genes and completeness of pedigree information were analyzed as well as the demographic structure of the population. Over the past 20 years, a steady increase in the population size has been observed. The share of different countries in Lowland wisent breeding also changed. The study showed that the accuracy of pedigree information decreased significantly between 1995 and 2015. In 1995 169 from 342 animals living in captivity had full pedigree information. In 2005 it was 76 from 320 animals. Within the population being alive in 2015, only 62 form 577 (10%) of all animals from Lowland line have full pedigree information, that leads to the founders of the population. An increase in the average values of inbreeding coefficient in the population and mean kinship was also observed.
The paper deals with the issue of rural problem areas. The author tries to present the possibility of an integrated approach to problem areas, showing different criteria of their delimitation as well as the time-space template. On the example of the Małopolska case study different categories and types of problem communes have been distinguished. The statistics referring to this area have also been shown.
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