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The aim of the study was to investigate the effect of some different environmental conditions prevailing during the development and ripening of Echinochloa crus-galli diaspores on their germination. Some seeds were tested in the autumn the same year, whereas others were divided into two groups: dispersed seeds and seeds within the inflorescence. Then the seeds of both groups were buried. After eight-month stratification in the soil, the diaspores were tested under the same conditions as the samples examined in the autumn. The seeds tested in the spring germinated faster than those tested in the autumn. Also the germination capacity of barnyard grass caryopses examined in the summer was almost twofold higher than the germination capacity of those examined in the autumn. Both autumn and spring tests revealed that the harvest time affected germination. The seeds obtained in the second half of August and at the beginning of September (in the middle of the growing season) were characterized by a higher germination capacity than the caryopses collected at the beginning and the end of the reproduction period. The results show that the germination capacity and rate were not influenced by the place of origin, habitat conditions and accompanying plants. It was found in spring tests that germination depended on the kind of dissemination unit stored in the soil. After eight-month soil stratification, dispersed caryopses germinated by approx. 20% better than those stored with a part of the inflorescence.
The paper describes structural and ultrastructural changes in primary explants, induction of embryogenesis, somatic embryo development, and four protocols for cryopreservation of cell suspensions. The changes during tissue culture of hypocotyl and cotyledon explants from 10-day-old seedlings and fragments of leaf explant of Gentiana cruciata L. were studied. Seedling explants were cultured on MS medium supplemented with 1.0 mg/l dicamba + 0.1 mg/l NAA + 2.00 mg/l BAP + 80.0 mg/l adenine sulphate. The hypocotyl callus tissue was initiated by cell divisions of the vascular cylinder, but in cotyledons only parenchyma cells took part in callus formation. The leaf blade expiants usually responded only by proliferation of the wounded surface. The effect of auxins (2,4-D, NAA, DIC) and cytokinins (kinetin, zeatin, BAP) in various concentration and combinations on leaf explant response was examined. Generally, embryos were formed sporadically on media containing NAA (1.64% responding explants) or 2,4-D (0.38%), but were not produced in the presence of dicamba. Production of somatic embryos was more effective from suspension culture than from agar medium. Liquid culture made it possible to maintain the cell suspension’s embryogenic competence for 5 years. For preservation of proembryogenic masses, four protocols of cryopreservation were studied: direct cooling, sorbitol/DMSO treatment, vitrification, and encapsulation. Direct cooling and sorbitol/DMSO treatment was unsuccessful. Vitrified tissue required a minimum 3 weeks of culture on solid medium for cell proliferation to reach the proper fresh weight for manipulation. Alginate beads with PEMs were transferred directly to liquid medium for post-freezing culture. Vitrification and encapsulation maintained high viability of post-freezing PEM, but encapsulation ensured faster restoration of G. cruciata cell suspension.
Trees are long living organisms, rarely used in molecular experiments because of large size of the genome and long time of reproduction cycle. Sequencing data from Populus trichocarpa genome allowed for the development of research on the processes associated with tree biology such as secondary wood formation, long-term perennial growth, seasonal changes, biotic interactions, evolution etc. Reference data enable the investigation of non-model trees such as Quercus or Fagus, having ecological and economic significance. During projects scientists use genomic, transcriptomic, proteomic and metabolomic approaches which contribute to better understanding of the physiological processes regulating tree biology. Data collected from these multiple studies need to be integrated. The integration of data is the subject of the newly established field of science called systems biology. This review presents progress in tree research after finishing the sequencing project of Populus. It concentrates on modern trends in 'omics' and systems biology study of temperate broadleave trees during the last 10 years of studies.
The paper discusses the results of rooting experiments conducted with the cuttings obtained from 8-year-old spruce trees of Istebna race. The studies investigated the effects of endogenous factors, such as the place of extraction, length and origin of cuttings, and an exogenous factor, which is a rooting stimulus.
Blackberries growing in forests regenerate mainly in a vegetative way; however, detailed studies have shown substantial variability in performance of Rubus individuals. The main problem is if the differences among plants are genetically based, or are due to site heterogeneity? This study was aimed at determining 1) how large are the differences in performance of individual plants and how persistent they are during consecutive years, 2) how much of that variability can be explained by the local environmental conditions, especially the intensity of competition among plants, and whether the performance of individual plants is related to their genetic characteristics. In a partially cut mature beech forest in Western Carpathians 35 randomly chosen individuals of Rubus hirtus (Waldst. & Kitt.) were tagged and measured each year for 9 years. Ten largest and ten smallest individuals were selected for genetic analysis. Mean size of individual plants differed between consecutive growing seasons, but the overall size ranking was quite consistent throughout the entire period of study. Analysis of environmental variables: relative light intensity and local abundance of potential competitors explained about 24% of the differences in individual plant performance. In the genetic analysis the five primers were applied to analyse all 20 samples. The five primers produced 10 polymorphic bands, showing a high variability. Only a few samples displayed identical band patterns, indicating either vegetative propagation or apomictic origin. Distribution of the results of Spearman’s rank correlation of plant size among genetically related individuals did not differ significantly from the distribution of rank correlation coefficients in the entire sample. In the analysed population genetical affinity did not seem to correlate with the performance of Rubus hirtus.
The interaction between seed plants and animals during pollination and fruit and seed dispersal is well known, and marks the sexual reproduction process. During the history of the plant kingdom, the development of sexual reproduction has been governed by changes in the environment of the plant, together with the increasing complexity of organisms. The interactions between gametes and the environment are prepared during gametogenesis, and therefore reproduction and dispersal are related from the beginning. The dynamic environment should be considered as an interactive partner. The more intensive interactions in multicellular organisms make the interaction in seed plants far more complex. Sexual reproduction plays a key role in the progress of the interaction between the dynamic environment and the biosphere. Sexual reproduction embodies the renewal and dispersal of organisms. This means that the interactions between organisms and their environment are not only an essential element of sexual reproduction but also a characteristic of life, based on the unity of organism and environment. The driving force of the increasing complexity of life is the dynamic environment and the persisting organism.
Sexual reproduction in angiosperms is an interactive process involving the sporophyte, gametophytes, embryo and endosperm as well as the environment, aimed at achieving pollination, fertilization and dispersal. This interaction occurs via an interface with nutrients and signals outside the cell and even outside the plant. Sexual reproduction has a history. In water, algae have different types of sex organs and gametes, and in some cases the female gamete stays on the plant. The zygote uses water movement and gravity for dispersal. Some algae have alternation of generations in the life cycle, and only the gametophyte functions in sexual reproduction. On land, ferns and mosses inherited alternation of generations, with oogamy and zygote development on the gametophyte, with wind dispersal of the meiospore. In angiosperms, heterospory and the retention of the megaspore, megagametophyte and embryo on the sporophyte lead to a seed with gravity and biotic dispersal. The history of sexual reproduction is based on sex determination, due to cross-fertilization and recombination. Sex differentiation is manifested in the increasing complexity of interaction in the nutrient supply, the retention of the gametophyte or even the embryo, and the type of vector of dispersal. Regulation of sexual reproduction in angiosperms is governed mainly by the sporophyte, with the expression of new genes for biotic pollination and seed dispersal. In the heterotrophic gametophyte some gene expression is suppressed. The development of sexual reproduction is due to the communication between the organism and a dynamic environment.
In the last few decades, changes of reproductive pattern of polar vascular plants have been observed, for the benefit of generative propagation. The reasons for this phenomenon are attributed to intensively following climate change, whose effects may be various. Warming causes the production of the greater number of generative structures, with higher quality. Our macroscopic observations conducted on specimens of polar vascular plants, cultivated in University of Warmia and Mazury greenhouse, indicate that the effect of temperature increase on flower development and seed formation is inconsistent. On the other hand enhanced levels of UV-B radiation can negatively affect seedlings. The complexity of the climate change causes tremendous difficulties in defining a clear and unquestioned way of modifications during the reproductive phase of the described plants.
We performed manipulative field experiments to investigate the effects of soil disturbance and exposure to a fungal plant pathogen, Puccinia coronata (Corda), on the establishment and spread of two introduced, cultivated genotypes of perennial ryegrass, Lolium perenne (L.). The two cultivars of L. perenne with different levels of susceptibility to P. coronata were introduced to grassland sites to investigate whether a plant cultivar selected to resist a pathogen shows better establishment in semi-natural plant communities than a susceptible cultivar. At two sites where L. perenne was already present, the addition of L. perenne seeds had no significant effect on the shoot biomass of the species, indicating that these populations were not seed limited. Exposure to the pathogen resulted in disease, and infected L. perenne populations showed increased shoot biomass over the course of the 3 year experiment and at harvest the final year, but no effect on seed production. Reproductive allocation was not affected by disease exposure in disturbed plots, but decreased in the presence of disease in undisturbed plots. The increased biomass observed in the semi-natural plant communities when exposed to the pathogen contrasts with the reduced biomass observed in garden experiments when the two cultivars of L. perenne were exposed to pathogen attack. The surprising positive effect of P. coronata on biomass in semi-natural communities indicates that processes here are more complex than in more intensively managed production systems.
A rare and protected species of the sedge Carex secalina was recorded in Dulsk near Inowrocław (Kujawy-Pomerania Province) in June 2014. It occupied the shores of ephemeral midfield water reservoirs in the complex of intensively used crop fields. Two subpopulations of this sedge with 36 tussocks in total were found. In the first subpopulation, the tussock diameter reached up to 25 cm, with 12–16 generative shoots per a tussock, while in the second subpopulation, the diameter was up to 10.5 cm, with 2–11 generative shoots. The rapid spread of this halophyte species is probably connected with the presence of permanent soil seed bank. This observation indicates a high potential of Carex secalina to colonise new habitats.
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