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Phenolic acids of walnut (Juglans regia L.)

86%
The chromatographic separations showed the presence of eleven free phenolic acids (trans-cinnamic, o-coumaric, p-coumaric, chlorogenic, ferulic, gallic, caffeic, p-hydroxybenzoic, syringic, tannic and vanillic) in the walnut tissues. The p-hydroxybenzoic acid was a predominant compound in the green husks (618.8 µg·g-1), though, high content of it was found in leaves obtained in July (478.3 µg·g-1). The content of chlorogenic acid in green husks of walnut (306.7 µg·g-1) was twice higher than that in the leaves obtained in July. Similar level of coumaric acids was determined in the leaves and green husks (o-coumaric: 42.4–48.2 µg·g-1; p-coumaric: 78.6–94.3 µg·g-1), though, o-coumaric acid was not found in May-leaves. Moreover, leaves of walnut possessed high content of caffeic acid (leaves obtained in May – 456.9; leaves obtained in July – 262.1 µg·g-1) and ferulic acid (leaves obtained in May – 93.5; leaves obtained in July – 292.5 µg·g-1). The predominant compound of the male inflorescences was vanillic acid (359.5 µg·g-1). The green husks of walnut were rich in polyphenolic acids, syringic (427.0 µg·g-1) and tannic (237.4 µg·g-1). However, the content of tannic acid was higher in the leaves - especially in leaves collected in May (350.7 µg·g-1). In tested tissues of walnut, gallic acid was found in the lowest concentration (male inflorescences – 32.7 µg·g-1and leaves obtained in July – 12.7 µg·g-1). In general, there was a great variation in a level of the phenolic acids between the studied organs of the walnut; nevertheless, walnut tissues are precious source of phenolic acids.
The aim of the study was to determine the effect of soil contamination with arsenic on the concentration of iron in some plant species. In addition, the following arsenic neutralising substances were added to soil for tests: lime, dolomite, natural and synthetic zeolite, charcoal, loam and compost. Soil contamination with arsenic, application of neutralising substances to the soil as well as the crop species had significant influence on the content of iron in the tested plant organs. Soil contamination with arsenic had an antagonistic or synergistic effect on the content of iron in plants, which was closely related with the crop species. In a series without neutralising substances added to the soil, arsenic had a negative effect on the content of iron in the above-ground parts and roots of cocksfoot, roots of maize, grain, straw and roots of spring barley, but a positive influence on its accumulation in the above-ground parts of maize and roots of swede. The highest increase in the content of iron was found in the above-ground parts of maize. The application of inactivating substances to the soil significantly modified the content of iron in the tested plant organs. These substances caused a decrease in the concentration of iron in the above-ground parts of maize and cocksfoot, but led to an increase in the accumulation of this element in straw and roots of spring barley. The direction of change in the concentration of iron in plants, i.e. decrease or increase, following the application of arsenic neutralising substances depended on plant species and the tested organ as well as the type of substance added to the soil.
In this study we propose a simple mathematical model based on the equilibrium equation for the materials deformed elastically. Owing to the turgor pressure of the cells, the peripheral walls of the outer tissue are under tension, while the extensible inner tissue is under compression. This well known properties of growing multicellular plant organs can be derived from the equation for equilibrium. The analytic solutions may serve as a good starting point for modeling the growth of a single plant cell or an organ.
During lateral root (LR) development a coordinate sequence of cell divisions, accompanied by a change of the organ form takes place. Both the order of anatomical events and morphological features may vary for individual primordia. At early stages of LR primordia development oblique division walls are inserted in cells that are symmetrically located on both sides of the axis of the developing LR primordium, and thereby allow for the protrusion of the LR. We hypothesize that both oblique cell wall insertion and continuous changes in primordium form could be a consequence of a local change in stress distribution in the region of the LR initiation.
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58%
This is a first part of work devoted highland rush endophytes: Penicillium expansum, Cladosporium oxysporum, Arthrinium state of Apiospora montagnei and Aureobasidium pullulans. The basidiomycete strain, possibly Lagarobasidium detriticum was also isolated.
The content and chemical composition of phenolic compounds in above- and underground organs of dropwort during second year of plant vegetation were studied. Five flavonoids (hyperosid, astragalin, spireaoside, kaempferol, quercetin), 2 catechin derivatives ((+)-catechin, (-)-epigallocatechin), and 7 polyphenolic acids (ellagic, gallic, syringic, salicylic, chlorogenic, caffeic and rosmarinic) were identified in aboveground organs. Their content, both in flowers and leaves, was significantly higher at the beginning of flowering as compared with full flowering stage. In underground organs (+)-catechin and its derivatives ((-)-epigallocatechin, (-)-epigallocatechin gallate, (-)-epicatechin) as well as 2 polyphenolic acids (ellagic and gallic) were identified. Their content was not closely related to the stage of plant development.
In this study, calcium oxalate (CaOx) crystals were investigated and their morphology and distribution determined by light microscopy in tissues and organs of Inula graveolens (L.) Desf., Pulicaria dysenterica (L.) Bernh., Filago eriocephala Guss., Logfia arvensis (L.) Holup and Logfia gallica (L.) Coss. & Germ., which belong to tribe Inuleae (Asteraceae). CaOx crystals were identified in cleared organs and tissues by a histochemical technique using silver nitrate and rubeanic acid. Druses were observed in stem pith cells, leaf mesophyll cells and style cells of I. graveolens. In anther tissues, crystals were determined as styloids, and in the ovary they were identified as prismatics. No crystals were found in petal and filament cells of I. graveolens. Druse crystals were present in the filament and style cells of P. dysenterica; styloids were found in the endothecial tissues of anthers, and prismatic crystals in the ovary cells of this species. No crystals were found in petal, stem and leaf tissues of P. dysenterica. F. eriocephala and L. arvensis, and L. gallica had small prismatic crystals only in their ovaries. No crystals were observed in the other tissues of these species. This study represents additional data on the presence of CaOx crystals in Asteraceae.
Wild berries of the genus Vaccinium have become increasingly popular in human health promotion due to their nutritional and medicinal properties. Some striking divergence of opinion about the content of triterpenoids in these plants still exists, meanwhile, this very large class of natural isoprenoids exhibits a wide range of biological activities and hence is of growing research interest. An investigation of triterpenoidal constituents from the cowberry (Vaccinium vitis-idaeaL.) plant led to the isolation of two isomeric acids:oleanolic and ursolic and the occurrence of their derivatives in this plant was demonstrated for the first time. Free triterpene acids as well as small amounts of their bound forms (presumable glycosides and glycoside esters) occur in fruits and the vegetative part of the plant, however, in various amounts and different ratios. The total content of both acids was the highest in organs regarded as traditional herbal resources, namely fruits and leaves (1 and 0.6% of dry mass, respectively), whereas it was markedly lower in stems and rhizomes. However, the rhizomes were in turn the plant organ containing relatively the highest amount of the bound forms of both acids (0.01% of dry mass). Ursolic acid was dominant in the whole plant, but the ratio of oleanolic to ursolic acid was significantly different in individual organs, decreasing from the upper (fruits 1:2.4, leaves 1:2) to the lower (stems 1:3.5, rhizomes 1:5.2) parts of the plant. This pattern of distribution of triterpenoids in the plant may have an important physiological and ecological meaning.
Thaumatin II is an extremely sweet-tasting protein produced by fruits of the West African shrub Thaumatococcus daniellii Benth, so it can be used in biotechnology to improve the tastes of various plant products. This study is concerned with the spatial and temporal aspects of expression of the 35S-pre-prothaumatin II chimeric gene in flower buds and fruits of transgenic cucumber (Cucumis sativus L.) line 225. The activity of the 35S promoter in organs of line 225 was compared with its activity in 2 other transgenic lines. The accumulation of recombinant thaumatin varied spatially in flower bud tissues of transgenic lines. We found that these differences in the spatial accumulation of transgenic protein concerned the ovary of female buds and the perianth of male buds. In contrast to flower parts, recombinant thaumatin was found in nearly all parts of the young fruit from the transgenic plants. The pre-prothaumatln II gene expression was detected at a very early developmental stage in male buds, and its pattern was rather conserved as the buds aged. The expression of the transgene was also detected in vascular tissues of examined organs but was undetectable in pollen grains, in agreement with the generally held view that the CaMV 35S promoter is virtually silent in pollen. Immunocytochemical analyses of sections of control organs revealed endogenous homolog(s) of thaumatin when using polyclonal antisera, but not when using monoclonal antibodies for recombinant thaumatin detection in transgenic cucumber.
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