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1

Ankyrin inhibits interaction of erythrocyte spectrin with phospholipids

100%
Bialkowska K., Sikorski A. F.
Biophysics of Membrane Transport
|
1994
|
tom 12
|
nr 2
2

Sphingosine, sphingosylphosphorylcholine and sphingosine 1-phosphate modulate phosphatidylserine homeostasis in glioma C6 cells

100%
Wojcik M., Baranska J.
Acta Biochimica Polonica
|
1999
|
tom 46
|
nr 1
The effect of sphingosine, sphingosylphosphorylcholine and sphingosine 1-phosphate on L-[U-14C]serine incorporation into phosphatidylserine and phosphatidylserine-derived phosphatidylethanolamine was investigated in intact glioma C6 cells. Sphingosine, sphingosylphosphorylcholine and sphingosine 1-phosphate are potent signalling molecules which, due to their physicochemical features, may function as amphiphilic compounds. It has been found that sphingosine and sphingosylphosphorylcholine (amphiphilic cations) significantly increase [14C]phosphatidylserine synthesis and decrease the amount of 14C-labeled phosphatidylethanolamine. Sphingosine 1-phosphate (an amphiphilic anion) was without effect on phosphatidylserine synthesis but, similarly as sphingosine and sphingosylphosphorylcholine, reduced the conversion of phosphatidylserine to phosphatidylethanolamine. These results strongly suggest that sphingosine, sphingosylphosphorylcholine and sphingosine 1-phosphate can modulate cellular phospholipid homeostasis by stimulation of phosphatidylserine synthesis and an interference with phosphatidylserine decarboxylase.
3

The effect of the lipid-binding site of the ankyrin-binding domain of erythroid beta-spectrin on the properties of natural membranes and skeletal structures

84%
Chorzalska A., Lach A., Borowik T., Wolny M., Hryniewicz-Jankowska A., Kolondra A., Langner M., Sikorski A. F.
Cellular and Molecular Biology Letters
|
2010
|
tom 15
|
nr 3
406-423
It was previously shown that the beta-spectrin ankyrin-binding domain binds lipid domains rich in PE in an ankyrin-dependent manner, and that its N-terminal sequence is crucial in interactions with phospholipids. In this study, the effect of the full-length ankyrin-binding domain of β-spectrin on natural erythrocyte and HeLa cell membranes was tested. It was found that, when encapsulated in resealed erythrocyte ghosts, the protein representing the full-length ankyrin-binding domain strongly affected the shape and barrier properties of the erythrocyte membrane, and induced partial spectrin release from the membrane, while truncated mutants had no effect. As found previously (Bok et al. Cell Biol. Int. 31 (2007) 1482–94), overexpression of the full-length GFP-tagged ankyrin-binding domain aggregated and induced aggregation of endogenous spectrin, but this was not the case with overexpression of proteins truncated at their N-terminus. Here, we show that the aggregation of spectrin was accompanied by the aggregation of integral membrane proteins that are known to be connected to spectrin via ankyrin, i.e. Na+K+ATP-ase, IP3 receptor protein and L1 CAM. By contrast, the morphology of the actin cytoskeleton remained unchanged and aggregation of cadherin E or N did not occur upon the overexpression of either full-length or truncated ankyrin-binding domain proteins. The obtained results indicate a substantial role of the lipid-binding part of the β-spectrin ankyrin-binding domain in the determination of the membrane and spectrin-based skeleton functional properties.
4

Transmembrane segment M2 of glycine receptor as a model system for the pore-forming structure of ion channels

84%
Bednarczyk P., Szewczyk A., Dolowy K.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 4
The glycine receptor belongs to the ligand-gated ion channel superfamily. It is a chlo­ride conducting channel composed of four transmembrane domains. It was previously shown that the second transmembrane domain (M2) of the glycine receptor forms an ion conduction pathway throught lipid bilayers. The amino-acid sequence of the transmembrane segment M2 of the glycine receptor has a high homology to all recep­tors of the ligand-gated ion channel superfamily. In our report, we have used a syn­thetic M2 peptide. It was incorporated into a planar membrane of known lipid compo­sition and currents induced by M2 were measured by the Black Lipid Membrane tech­nique. When the planar lipid bilayer was composed of 75% phosphatidylethanolamine and 25% phosphatidylserine, the reversal potential measured in a 150/600 mM KCl (cis/trans) gradient was -19 mV suggesting that the examined pore was preferential to anions, Pk/Pci = 0.25. In contrast, when 75% phosphatidylserine and 25% phosphatidylethanolamine was used, the reversal potential was +20 mV and the pore was preferential to cations, Pk/Pci = 4.36. Single-channel currents were recorded with two predominant amplitudes corresponding to the main-conductance and sub-conductance states. Both conductance states (about 12 pS and 30 pS) were mea­sured in a symmetric solution of 50 mM KCl. The observed single-channel properties suggest that the selectivity and conductance of the pore formed by the M2 peptide of the glycine receptor depend on the lipid composition of the planar bilayer.
5

Interaction of spectrin with phospholipids is inhibited by isolated erythrocyte ankyrin. A monolayer study

84%
Bialkowska K., Lesniewski J., Nietubyc M., Sikorski A. F.
Cellular and Molecular Biology Letters
|
1999
|
tom 04
|
nr 2
It was previously found (Białkowska, K., Zembroń, A. and Sikorski, A. F. (1994) Biochim. Biophys. Acta 1191, 21-26.) that isolated erythrocyte ankyrin inhibited interaction of spectrin with phospholipid liposomes, mainly those prepared from lipid mixtures containing aminophospholipids. In this communication we report on the effect of isolated ankyrin on red blood cell spectrin interaction with phospholipids in monolayers. Our data indicate that spectrin interaction with monolayers containing PE and, to a smaller extent, PS is sensitive to inhibition by ankyrin while interaction with monolayers containing only PC is not. Tetrameric spectrin affects monolayer surface pressure similarly to the heterodimer. However, an interaction of tetrameric spectrin with phospholipids was much more effectively inhibited by purified ankyrin indicating that one site per spectrin tetramer engaged in this interaction. When interactions of purified individual spectrin subunits (α or β) with phospholipid monolayers was studied, the β-subunit caused a strong, saturable effect on the surface pressure of the PE/PC monolayer, in contrast to the α-chain which induced much smaller and monotonic changes on the surface pressure of the same monolayer. Also interactions of the β-subunit with amino-phospholipids/PC monolayers were more sensitive to inhibition by ankyrin than those with native αβ-heterodimer of spectrin, e.g. threefold lower concentration of ankyrin was necessary to induce the same effect, while interaction of the α-subunit with phospholipid monolayers was entirely insensitive to ankyrin. Phosphorylation of spectrin in vitro with either cAMP-dependent protein kinase, or metabolically in intact cells, induced a decrease in the effect of either dimer or tetramer on the surface pressure of phospholipid monolayers. The sensitivity of this interaction to ankyrin was also greatly diminished. When isolated ankyrin was phosphorylated by the same cAMP-dependent protein kinase its ability to compete with phospholipid for spectrin was also diminished. The described effects may indicate a physiological significance of spectrin’s interaction with phospholipids, particularly in situations when there is not enough functional ankyrin to accommodate all spectrin molecules in the membrane.
6

Construction and optimisation of a computer model for a bacterial membrane

84%
Murzyn K., Pasenkiewicz-Gierula M.
Acta Biochimica Polonica
|
1999
|
tom 46
|
nr 3
The main steps in the construction of a computer model for a bacterial membrane are described. The membrane has been built of 72 lipid molecules, 54 of which being 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphatidylethanolamine (POPE) and 18 - 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphatidyl-rac-glycerol (POPG) molecules (thus in the proportion of 3:1). The membrane was hydrated with 1955 water molecules (approximately 27 water molecules per lipid). To neutralise the electronic charge (-e) on each POPG molecule, 18 sodium ions (Na+) were added to the membrane close to the POPG phosphate groups. The atomic charges on the POPE and POPG headgroups were obtained from ab initio quantum mechanical restrained electrostatic potential fitting (RESP) (Bayly et al., 1993, J. Phys. Chem. 97, 10269) using the GAMESS program at the 6-31G* level (Schmidt et al., 1993, J. Comput. Chem. 14, 1347). The model constructed in this way provided an initial structure for subsequent molecular dynamics simulation studies intended to elucidate the atomic level interactions responsible for the structure and dynamics of the bacterial membrane.
7

Interactions of spectrins with phospholipids: possible physiological role

84%
Sikorski A. F.
Cellular and Molecular Biology Letters
|
1998
|
tom 03
|
nr 2
8

The induction of cytochrome P450 isoform, CYP4A1, by clofibrate coincides with activation of ethanolamine-specific phospholipid base exchange reaction in rat liver microsomes

84%
Lenart J., Komanska I., Jasinska R., Pikula S.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 1
Administration of a hypolipidaemic drug, clofibrate, to rats resulted, 24 h after a single intraperitoneal injection (250 mg/kg body weight), in pronounced enhancement of the rate of phosphatidylethanolamine (PE) synthesis via the PE-specific base exchange (PEBE) reaction in liver microsomes. This was accompanied by 3.4-fold activation of microsomal ω-hydroxylation of lauric acid by cytochrome P450 4A1 isoform (CYP4A1) and an increase in the protein content of this isoform in endoplasmic reticulum (ER) membranes. Since PE represents a class of phospholipids (PL) prerequisite for proper functioning of CYP4A1, and the PEBE reaction is an inducible pathway of PL synthesis in hepatocytes under metabolic stress, one may speculate that this reaction is switched on when extensive remodelling of PL molecular species or/and massive synthesis of lipid bilayer components for membrane assembly is required.
9

Comparing the content of lipids derived from the eye lenses of various species

67%
Panz T., Lepiarczyk M., Zuber A.
Folia Histochemica et Cytobiologica
|
2011
|
tom 49
|
nr 3
10
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Effect of triiodothyronine on the content of phospholipids in the rat liver nuclei

67%
Bucki R., Gorska M., Zendzian-Piotrowska M., Gorski J.
Journal of Physiology and Pharmacology
|
2000
|
tom 51
|
nr 3
11

Interaction of isolated erythrocyte membrane skeletons with phospholipids

67%
Sikorski A. F., Zielinski B., Wroblewski Z., Kozubek A.
Current Topics in Biophysics
|
1995
|
tom 19
|
nr 1
12

Molecular dynamics simulation studies of lipid bilayer systems

67%
Pasenkiewicz-Gierula M., Murzyn K., Rog T., Czaplewski C.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 3
The main structural element of biological membranes is a liquid-crystalline lipid bilayer. Other constituents, i.e. proteins, sterols and peptides, either intercalate into or loosely attach to the bilayer. We applied a molecular dynamics simulation method to study membrane systems at various levels of compositional complexity. The studies were started from simple lipid bilayers containing a single type phosphatidylcholine (PC) and water molecules (PC bilayers). As a next step, cholesterol (Chol) molecules were introduced to the PC bilayers (PC-Chol bilayers). These studies provided detailed information about the structure and dynamics of the membrane/water interface and the hydrocarbon chain region in bilayers built of various types of PCs and Chol. This enabled studies of membrane systems of higher complexity. They included the investigation of an integral membrane protein in its natural environment of a PC bilayer, and the antibacterial activity of magainin-2. The latter study required the construction of a model bacterial membrane which consisted of two types of phospholipids and counter ions. Whenever published experimental data were available, the results of the simulations were compared with them.
13

Effect of vegetable oils on the composition of fatty acids in the rat livers and 7,12-dimethylbenz[a]anthracene [DMBA] - induced tumours

67%
Tokarz A., Jelinska M., Oledzka R., Czorniuk-Sliwa A.
Acta Poloniae Toxicologica
|
2001
|
tom 09
|
nr 1
14

Prostaglandin E [dmPGE2] action in vitro on the activity of rat liver Golgi apparatus galactosyltransferase

67%
Kordowiak A. M., Tomecki J., Procyk K., Kapusta P.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 4
In vitro addition of 16,16'-dimethyl prostaglandin E2 to Golgi-rich membrane fraction in final concentration of 0.1 ng/1 mg of protein increased generally the activity of galactosyltransferase in comparison with control. The percentage of phospholipids in the whole fraction was similar in both investigated groups, only the sum of phosphatidylethanolamine + phosphatide acid was significantly lower after addition of dmPGE2 than in the control (0.001
15

Interaction of spectrins with membrane intrinsic domain

67%
Sikorski A. F., Bialkowska K.
Cellular and Molecular Biology Letters
|
1996
|
tom 01
|
nr 1
Although interaction of red blood cell spectrin with phospholipids of the membrane bilayer was first reported more than 20 years ago its physiological significance is still a matter of dispute. In this paper we review the available data on interactions of red blood cell and nonerythroid spectrin with membrane phospholipids. The results from our laboratory indicate that ankyrin and phospholipids (particularly PE1-rich lipid domains) may, at least in part, share a binding site on erythrocyte and brain spectrin b-subunit. The physiological significance of this model is discussed.
16

Evidence for the presence of the Kennedy and Bremer-Greenberg pathways in Caenorhabditis elegans

67%
Lochnit G., Heyer R.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 4
Nematodes were found to synthesize phosphorylcholine-containing molecules not present in higher organisms, i.e. phosphorylcholine-substituted glycosphingolipids and (glyco)proteins. Investigations on the biosynthesis of these structures provided first biochemical evidence for the presence of the Kennedy and Bremer-Greenberg pathways in the model organism Caenorhabditis elegans.
17

Investigation of interaction of human platelet membrane components with anticoagulant drugs abciximab and eptifibatide

59%
Gorodkiewicz E., Sankiewicz A., Figaszewski Z.
Folia Histochemica et Cytobiologica
|
2010
|
tom 48
|
nr 4
18

Temporal dynamics and regional distribution of [14 C] serine uptake into mouse brain

59%
Woronczak J. P., Siucinska E., Kossut M., Baranska J.
Acta Neurobiologiae Experimentalis
|
1995
|
tom 55
|
nr 4
19

Synthesis of aminophospholipids in Saccharomyces cerevisiae and Chinese hamster ovary cells. From mutagenesis to genes and cellular function

59%
Lenart J., Pikula S.
Cellular and Molecular Biology Letters
|
1998
|
tom 03
|
nr 2
The purpose of this article is to provide a concise overview of the characterization of auxotrophic mutated cells to the precursors of lipid synthesis, and of the identification of specific genes encoding enzymatic proteins involved in this process. The focus is on enzymes catalyzing the synthesis of phosphatidylserine and phosphatidylethanolamine in Saccharomyces cerevisiae and Chinese hamster ovary cells, two cell types frequently used by investigators studying the mechanisms of genetic control of metabolic processes.
20

Plasma membrane phospholipid asymmetry and its maintenance

51%
Roelofsen B., Vermeulen W. P., Op den Kamp J.A.F.
Biophysics of Membrane Transport
|
1994
|
tom 12
|
nr 2
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