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Fractionation of buckwheat seed phenolics and analysis of their antioxidant activity

100%

Karamac M., Biskup I., Kulczyk A.

Polish Journal of Food and Nutrition Sciences

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2015

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tom 65

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nr 4

Five fractions of phenolic compounds were obtained from the extract of common buckwheat seed (Fagopyrum esculentum Moench) using Sephadex LH-20 column chromatography with methanol as a mobile phase. The total phenolics content ranged from 19.8±1.5 (fraction I) to 164±2.2 mg (+)-catechin eq/g (fraction IV). The profiles of phenolic acids and flavonoids in the fractions were analysed using RP-HPLC-DAD. The antioxidant activity was tested as ABTS^+ and DPPH scavenging activity and capability to reduce the Fe(III)/2,4,6-Tris(2-pyridyl)-s-triazine complex to the ferrous form. Results were expressed as Trolox equivalent antioxidant capacity (TEAC), IC50 and ferric reducing antioxidant power (FRAP) values, respectively. The highest antioxidant activity was noted for fraction IV that was predominated by flavones. TEAC, IC50 and FRAP values were: 1.47±0.01 mmol Trolox eq/g, 0.058±0.003 mg/assay and 2.18±0.05 mmol Fe(II)/g, respectively. Rutin constituted 77.7% of the compounds identified in fraction III. The antiradical activity and reducing capability of this fraction were lower compared to fraction IV, but significantly higher than in fractions I and II. The main phenolic compounds of fractions I and II were phenolic acids (caffeic, 5-O-caffeoylquinic and p-coumaric). The antioxidant activity of fraction V was similar to that of fraction III.

2

Characterization and quantitative analysis of procyanidins in fruits of hawthorn species (Crataegus spp.)

100%

Liu P., Kallio H., Yang B.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

3

Antioxidant activity, phenol and flavonoid contents of Portuguese shrub: Erica australis

100%

Carvalho I. S., Nunes R. S.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

|

nr Suppl.1

4

Phenolics in undifferentiated tissue cultures of Cichorieae

100%

Malarz J., Stojakowska A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

5

The antimicrobial activity of Prunella vulgaris extracts

100%

Mahboubi M., Mahboubi A., Kazempour N.

Herba Polonica

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2015

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tom 61

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nr 1

Prunella vulgaris ( Labiatae family) or self-heal is traditionally used for different ailments such as eye pain and inflammation, headache, dizziness, sore throat and wound healing. Total phenolic and total flavonoid contents of extracts (methanol, ethanol and aqueous) were determined by a spectrophotometer. The antimicrobial activity was evaluated by micro broth dilution assay. The total phenolic content of P. vulgaris extracts were higher in aqueous extract (156.5 mg GAC/g) followed by ethanol extract and methanol extract. The TFC content of P. vulgaris methanol extract (82.8 mg QE/g) was higher than ethanol extract (22.7 mg QE/g) and aqueous extract (16.2 mg QE/g). The antimicrobial activity of methanol or ethanol extracts was higher than aqueous extract from P. vulgaris. The sensitivity of microorganisms to different extracts is related to type of pathogens. There is no positive relation between total phenolic content and its antimicrobial activity. Prunella vulgaris ethanolic extract as a source of phenolic and flavonoid contents can be used as an antimicrobial agent

6

Effect of soil sieving on respiration induced by low-molecular-weight substrates

100%

Datta R., Vranova V., Pavelka M., Rejsek K., Formanek P.

International Agrophysics

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2014

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tom 28

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nr 1

The mesh size of sieves has a significant impact upon soil disturbance, affecting pore structure, fungal hyphae, proportion of fungi to bacteria, and organic matter fractions. The effects are dependent upon soil type and plant coverage. Sieving through a 2 mm mesh increases mineralization of exogenously supplied carbohydrates and phenolics compared to a 5 mm mesh and the effect is significant (p<0.05), especially in organic horizons, due to increased microbial metabolism and alteration of other soil properties. Finer mesh size particularly increases arabinose, mannose, galactose, ferulic and pthalic acid metabolism, whereas maltose mineralization is less affected. Sieving through a 5 mm mesh size is suggested for all type of experiments where enhanced mineralization of low-molecular-weight organic compounds needs to be minimalized.

7

Phenolic compounds and antioxidant activity of Castanopsis phuthoensis and Castanopsis grandicicatricata

100%

Tuyen P. T., Khang D. T., Minh L. T., Minh T. N., Ha P. T. T., Elzaawely A. A., Xuan T. D.

International Letters of Natural Sciences

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2016

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tom 55

In this study, total phenolic and flavonoid contents, antioxidant capacity, and phenolic compositions of Castanopsis phuthoensis and Castanopsis grandicicatricata (Fagaceae family) were investigated. It was found that bark extracts were rich of phenolic contents, whereas leaf extracts were abundant of flavonoids. The total phenolics varied from 11.20 to 35.47 mg gallic acid equivalent g-1 dry weight (DW), and the total flavonoids were from 2.24 to 12.55 mg rutin equivalent g-1 DW. The results of antioxidant activity evaluation showed that the DPPH (1,1- diphenyl-2-picryl-hydrazyl) radical scavenging activity of the free phenolic extracts were higher than the bound phenolic extracts. Regarding the reducing power and β-carotene bleaching assays, the free phenolic extracts showed remarkably strong antioxidant capacity that were similar to the levels of the standard BHT (dibutyl hydroxytoluene) did. It could be concluded that free phenolic extracts were more effective in antioxidant activities than bound phenolic extracts. Highly significant correlations were observed between phenolic contents and antioxidant activities (0.813 for DPPH and 0.841 for reducing power). By HPLC analysis, seven phenolic acids were detected including gallic, p-hydroxybenzoic, vanillic, sinapic, p-coumaric, ellagic acids, and vanillin. Of which, gallic, ellagic, and sinapic acids were the most abundant compounds in the two species. The results suggest C. phuthoensis and C. grandicicatricata contain rich sources of natural antioxidants and phenolic compounds which are probably considered in pharmaceutical use.

8

Total phenolics variation in leaves of some Hieracium L. and Pilosella Hill. species

100%

Ayaz F. A., Coskuncelebi K.

Acta Biologica Cracoviensia. Series Botanica

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2002

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tom 44

Mature leaves of 20 Hieracium L. and 11 Pilosella Hill. species collected from the alpine and subalpine regions of northeast Anatolia (Turkey) were examined for total phenolic content. The highest level was found in leaves of H. conicum (22.5 mg phenol equiv/g dry wt) and the lowest H. cardiophyllum (3.7 mg phenol equiv/g dry wt). Total phenolics content varied significantly (p = 0.05) among species of Hieracium and in decreasing order as follows: H. conicum (22.5) > H. hypoglaucum (21.5) > H. amblylepis (18.1) > H. subsilvularum (17 .2) > H. jurassicum (15.1) >H. tamderense (14.8) > H. mannagettae (14.7) > H. cardiophyllum (3 .7). In Pilosella the level ranged between 5.6 and 25.5 mg phenol equiv/g dry wt, with the lowest in P. grossheimii and the highest in P. hypeuryum. Total phenolics content varied significantly (p = 0.05) among species of Pilosella, in decreasing order: P. hypeuryum (22.5) > P. tephrocephala (17.9) > P. officinarum (15.7) > P. macranthum (13.7) > P. fennica (10.5) > P. macrotricha (7.7) > P. grosheimii (5.6). Within genera, 40% of the Hieracium species and ~64% of the Pilosella species significantly differed in total phenolics content, while ~19.4% of the species significantly differed between genera. The range of differences in total phenolics content in the genera was not wide, clearly due to their taxonomically close relationships between species.

9

Content of phenolics in mycorrhizal roots of Pinus sylvestris seedlings grown in vitro

100%

Napierala-Filipiak A., Werner A., Karolewski P.

Acta Physiologiae Plantarum

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2002

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tom 24

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nr 3

10

Methods to determine antioxidative capacity in fruit

100%

Vangdal E., Slimestad R.

Journal of Fruit and Ornamental Plant Research

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2006

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tom 14

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nr Suppl.2

11

Phenolics in apples and processed apple products

100%

Markowski J., Kolodziejczyk K., Krol B., Plocharski W., Rutkowski K.

Polish Journal of Food and Nutrition Sciences

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2007

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tom 57

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nr 4[B]

12

Antioxidant capacity of roasted health-promoting products

100%

Kosinska A., Karamac M.

Polish Journal of Food and Nutrition Sciences

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2006

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tom 15/56

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nr 2

Roasted sesame seeds, pumpkin seeds, sunflower seeds, soy beans, and wheat germs were obtained from Ekoprodukt company (Częstochowa, Poland). Phenolic compounds were extracted with 80% aqueous methanol. The extract of roasted sunflower seeds was characterised by a higher content of total phenolics (158 mg/g). The Total Antioxidant Capacity was the highest in the extract of roasted sunflower seeds (0.478 mmol Trolox/g) followed by the extract of roasted wheat germs (0.066 mmol Trolox/g). The extracts of roasted sunflower seeds and wheat germs were strong scavengers of DPPH radical. Their reducing power was strong as well.

13

Effect of faba beans coat with different phenolics content on the use of protein by rats

100%

Zdunczyk Z., Frejnagel S., Krefft B.

Polish Journal of Food and Nutrition Sciences

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1996

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tom 05

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nr 2

The aim of the study was to determine the content and biological activity of phenolic compounds of three faba bean genotypes - a coloured flowered Nadwiślański variety, a coloured flowered "self-ending" Tinos variety and white flowered Caspar variety. Comparing the white flowered Caspar variety containing 0.34% of phenolic compounds in the seed coat, in this proantho- cyanidins made from 0.04%, the Polish Nadwiślański and Tinos variety were characterised as having a higher phenolic compound content in the seed coat, i.e. 8.70% and 11.75%, respectively. The proanthocyanidins content, being 6.54% and 8.32% respectively, is in accordance with the lower values determined in the seed coats of many European high-tannin faba bean varieties. Seed coats containing a higher level of polyphenols, added to casein diets in amounts of 15%, highly significantly lowered the digestibility and protein efficiency. Depending on the variety of seed coat, TD protein coefficients amounted to: Caspar — 85.4%, Nadwiślański — 71.3%, Tinos — 69.3%. For these same diets the NPU index was 71.2, 59.3 and 49.4, and the PER index was 2.63, 2.23 and 2.16, respectively. It was also found highly significant decrease of Ca, Fe, and Zn apparent digestibilities in casein diets with faba bean seed coats of a high polyphenolic content.

14

The first report on alternate bearing of barberry (Berberis vulgaris L.): change in total carbohydrate and phenolic contents

84%

Khayyat M., Arefnezhad Z., Zahan M. H. S., Zamani G.

Journal of Horticultural Research

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2018

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tom 26

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nr 1

15

Content of some chemical components in carrot (Daucus carota L.) roots depending on growth stimulators and stubble crops

84%

Kwiatkowski C. A., Haliniarz M., Kolodziej B., Harasim E., Tomczynska-Mleko M.

Journal of Elementology

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2015

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tom 20

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nr 4

Agronomic factors, and mineral fertilization in particular, have a strong impact on the quality of carrot roots. In recent years, researchers have begun to design eco-friendly agricultural practices that would stimulate the quality and yield of carrot roots. Our aim has been to analyse selected quality parameters pertaining to the chemical composition of carrot roots, and relate them to the application of different growth stimulators and cover crops. For this purpose, a field experiment was conducted in 2009-2011 investigating the effect of growth stimulators and stubble crops on the accumulation of chemical components in storage roots of carrot (cv. Laguna F1). The study included three growth stimulators: Asahi SL, Bio-algeen S 90 and Tytanit. Plots without any foliar application of these growth stimulators served as control treatment. Another factor consisted of the previous crop (spring barley) and stubble crops: tansy phacelia and a mixture of spring vetch and field pea, grown after the harvest of barley and then ploughed in. All the growth stimulators significantly reduced the NO3 content in carrot roots. Asahi SL significantly increased the phenolic content. The effects of the growth stimulators on the macronutrient content in carrot roots were statistically significant in the case of Mg and Na. Stubble cropping contributed to the reduction of nitrates and stimulated an increase in the content of phenolics as well as of P, K, Ca and Mg in carrot roots.

16

Antimicrobial and antioxidant activity of Epimedium pinnatum

84%

Mahboubi M., Kazempour N., Hosseini H., Mahboubi M.

Herba Polonica

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2013

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tom 59

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nr 2

Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity.

17

Influence of ripening time on fruit chemical composition of two blue honeysuckle cultigens

84%

Skupien K., Ochmian I., Grajkowski J.

Journal of Fruit and Ornamental Plant Research

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2009

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tom 17

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nr 1

101-111

The aim of 3 -year experiment was to evaluate quality and chemical composition of blue honeysuckle berries cultivar 'Czarna' and non-specified seedling "N" as dependent on time of ripening. The results obtained showed that berries harvested later were bigger but less firm. Furthermore, an increase of soluble solids content was observed in fruit of both cultigens at the end of harvest (for seedling "N" from 11 to 13.5% and for 'Czarna' from 9.8% to 12.6%). On the other hand, for late-harvested berries of both cultigens 20% decrease of titratable acidity and vitamin C was noticed. In berries of seedling "N" at the beginning of harvest 232 mg 100 g-1 of total polyphenols was determined, whereas the late ripening fruit contained 284 mg 100 g-1 of total polyphenols. Similar relation was observed in 'Czarna' berries (an increase from 164 mg 100 g-1 to 221 mg 100 g-1). The berries of late harvest contained more antho- cyanins (167 mg 100 g-1 in 'Czarna' and 227 mg 100 g-1 in seedling "N") as compared to early collected ones (122 and 173 mg 100 g-1 for, 'Czarna' and "N", respectively). Hydroxycinnamic acids content varied from 37.0 to 29.3 mg 100 g-1, respectively, for early and late collected berries of seedling "N" and from 22.3 mg 100 g-1 to 26.6 mg 100 g-1 for early and late harvested berries of 'Czarna'. Among flavonols, quercetin-3-rutinoside, quercetin-3-glucoside and two unidentified flavonols with retention times 26 min and 32 min, were determined. Luteolin 7-O-a-glucoside content varied from 4.59 to 7.05 mg 100 g-1 in the early and late harvested berries of seedling "N" and from 3.12 mg 100 g-1 in the early to 4.25 mg 100 g-1 in the late harvested berries of 'Czarna'.

18

Seasonal distribution of phenolics in leaves of aromatic plants (Origanum vulgare L., Mentha spicata L., Clinopodium vulgare L.) and their ecophysiological implications

84%

Kofidis G., Bosabalidis A. M.

Biological Letters

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2012

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tom 49

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nr 1

19

Influence of substrate on yield and chemical composition of highbush blueberry fruit cv. 'Sierra'

84%

Ochmian I., Grajkowski J., Skupien K.

Journal of Fruit and Ornamental Plant Research

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2009

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tom 17

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nr 1

89-100

The objective of the study carried out during 2004-2007 was to evaluate the influence of three types of substrates (peat, sawdust and cocoa husk) on yield, quality and chemical composition of highbushblueberries cv. 'Sierra'. On 4-year average, the lowest yield per a bush (0.89 kg) was obtained from bushes grown in cocoa husk substrate and these berries had the lowest weight of 100 fruit (64 g) and were the smallest. On the other hand, they had the highest firmness, measured both vertically (512 G mm-1) and horizontally (275 G mm-1). The highest soluble solids content (14.45%) and titratable acidity (1.02 g of citric acid equivalents 100 g-1 ) were found in fruit harvested from bushes growing in peat. The substrates did not affect significantly total sugar (11.98-12.30 g 100 g-1) and vitamin C contents (26.0-27.9 mg 100 g-1). The nitrates content in blueberries was low, ranging from 17.5 (peat) to 34.7 mg kg-1 (sawdust) whereas the content of nitrites varied from 0.85 (peat) to 1.10 mg kg-1 (sawdust). Blueberries obtained from peat- and cocoa husk- bedded bushes had significantly higher total phenolics content (231.03 mg and 222.29 mg per 100 g-1, respectively) compared to these grown in sawdust (181.74 mg 100 g-1). Similarly, the berries originating from peat- and cocoa husk- grown plants showed higher total anthocyanin content (144.55 mg 100 g-1 and 146.73 mg 100 g-1, respectively) than fruit collected from sawdust-bedded bushes (120.92 mg 100 g-1). Taking into account the yield alone, the best effects were obtained from 'Sierra' bushes grown in sawdust. However, regarding chemical composition, the berries from bushes grown in peat and cocoa husk were richest in nutritional components and, especially, in phenolic compounds.

20

Effects of honey addition on antioxidative properties of different herbal teas

84%

Toydemir G., Capanoglu E., Kamiloglu S., Firatligil-Durmus E., Sunay A. E., Samanci T., Boyacioglu D.

Polish Journal of Food and Nutrition Sciences

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2015

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tom 65

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nr 2

Tea and herbal infusions are among the major contributors of phenolic compounds, specifically flavonoids, in our daily diet. Honey is another antioxidant-rich food that is widely used as a natural sweetener. In this work, the effects of honey addition on antioxidant properties of different herbal teas were investigated. For this purpose, 2 different types of honey (flower and pine honey) were added into 9 different herbal teas (melissa, green tea, rosehip, sage, echinacea, fennel, linden, daisy, and ginger) at 4 different temperatures (55°C, 65°C, 75°C, and 85°C), and the changes in the content of total pheolics, total flavonoids, and total antioxidant capacity were determined. The total phenolic content and the total antioxidant capacity of the honey-added-tea samples were found to be increased (up to 57% for both), especially with pine honey and at higher temperatures of honey addition. The findings of this study supported the use of honey as a natural sweetener in tea in order to be able to benefit from the health-enhancing antioxidative properties of these two promising food products.

Ograniczanie wyników

Fractionation of buckwheat seed phenolics and analysis of their antioxidant activity

Characterization and quantitative analysis of procyanidins in fruits of hawthorn species (Crataegus spp.)

Antioxidant activity, phenol and flavonoid contents of Portuguese shrub: Erica australis

Phenolics in undifferentiated tissue cultures of Cichorieae