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The purpose of the project was to study the correlation between the presence of the IHN virus and the presence of antibodies against this virus in fish sera. Experimental infection of rainbow trout was performed at temperatures of 12°C±1 and 15°C±1. The trouts’ sera were collected at 7-day intervals. Samples from farms naturally infected with IHNV were also tested. The presence of antibodies against IHNV was determined by seroneutralization and the virus was detected by isolation in cell culture. It was found that in the experimentally infected rainbow trout, antibodies against IHN were found earlier in the higher temperature, after 21 days post infection, whereas the presence of IHNV was observed to last longer in the lower temperature. In the study performed on 30 samples of sera from IHN-infected farms, antibodies were detected in 12 samples. Detection of antibodies against IHNV in fish serum can be a helpful tool supporting recommended techniques.
The aim of this study was to estimate the prevalence of equine arteritis virus infections in horses in eastern Poland using the SN assay and the ELISA test. Sera of 442 horses were examined. In the SN assay, antibodies to EAV were detected in 185 samples with a titer range of 1:8 to 1:512. All sera samples examined by the SN test were analyzed again by the ELISA test. In this study positive reactions were observed for 136 out of 442 samples examined. Sera of 10 horses which were negative in the SN assay gave a positive reaction in ELISA. On the other hand, 59 sera negative in the ELISA test were positive in the SN assay with titers of 1:8 or higher. The ELISA test detected 49 seropositive animals less than the SN assay. Statistical analysis of the results of this study shows that the ELISA test, as compared with the SN assay, has a sensitivity of 68.1 % and specificity of 96.1%.
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