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1

The nucleotide sequence of 5s rRNA from bovine liver

100%

Szymanski M., Nalaskowska M., Barciszewski J.

Acta Biochimica Polonica

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1992

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tom 39

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nr 3

We have determined the nucleotide sequence of ribosomal 5S RNA from bovine liver. The comparison of this sequence with those from other cukaryotic sources shows that a common secondary structure model for all eukaryotic 5S rRNAs may exist Analysis of the evolutionary conserved nucleotides in metazoan 5S rRNAs suggests that the tertiary interactions, proposed earlier for plant 5S rRNA, are also possible.

2

Nucleotide probes of DNA polymerases

88%

Wright G. E.

Acta Biochimica Polonica

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1996

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tom 43

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nr 1

The modified nucleotides, N2-(p-n-butylphenyl)dGTP and 2-(p-n-butylanilino) dATP and related compounds have been developed as inhibitor-probes of B family DNA polymerases. Synthetic approaches to these compounds are summarized. The nucleotides are potent, non-substrate inhibitors of DNA polymerase a. In contrast, they inhibit other members of the family with less potency but act as substrates for these enzymes. Modelling of the inhibitor: enzyme binding mechanism has been done based on the known structure of E. coli DNA polymerase I, and site-directed mutagenesis experiments to evaluate this mechanism are proposed.

3

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MicroRNA-26a-5p: multiple functions, multiple possibilities – a mini-review

75%

Zapolnik P., Zapolnik B.

Journal of Pre-Clinical and Clinical Research

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2020

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tom 14

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nr 4

4

A new approach to phosphorylation of nucleosides using oxyonium phosphobetaines as intermediates

75%

Materna M., Stawinski J., Sobkowski M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2023

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tom 104

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nr 1

5

Annexin VI, a novel ATP-dependent protein. Alterations of phospholipid binding properties of annexin VI by nucleotides

75%

Danieluk M., Sikorski A. F., Pikula S., Bandorowicz-Pikula J.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 2

6

The effect of Arg209 to Lys mutation in mouse thymidylate synthase

75%

Ciesla J., Golos B., Walajtys-Rode E., Jagielska E., Plucienniczak A., Rode W.

Acta Biochimica Polonica

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2002

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tom 49

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nr 3

Mouse thymidylate synthase R209K (a mutation corresponding to R218K in Lactobacillus casei), overexpressed in thymidylate synthase-deficient Escherichia coli strain, was poorly soluble and with only feeble enzyme activity. The mutated protein, incubated with FdUMP and N5,10-methylenetetrahydrofolate, did not form a complex stable under conditions of SDS/polyacrylamide gel electrophoresis. The reaction catalyzed by the R209K enzyme (studied in a crude extract), compared to that catalyzed by purified wild-type recombinant mouse thymidylate synthase, showed the Km value for dUMP 571-fold higher and Vmax value over 50-fold (assuming that the mutated enzyme constituted 20% of total crude extract protein) lower. Thus the ratios kcat,R209K/kcat,'wild' and (kcat, R209K/Km, R209K dUMP)/ (kcat, 'wild'/Km, 'wild' dUMP) were 0.019 and 0.000032, respectively, documenting that mouse thymidylate synthase R209, similar to the corresponding L. casei R218, is essential for both dUMP binding and enzyme reaction.

7

Intron-containing human pre-tRNA Leu is a substrate for m5C34-methyltransferase

75%

Karwowska U., Szweykowska-Kulinska Z.

Biological Bulletin of Poznań

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1998

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tom 35

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nr 1

8

A single nucleotide polymorphism in the 5'untranslated region of RAD51 in breast cancer

75%

Blasiak J., Przybylowska K., Czechowska A., Zadrozny M., Rykala J., Kolacinska A., Morawiec Z., Drzewoski J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

9

Different responses for Ap5A and ATP in human cerebrocortical synaptic terminals

75%

Pintor J., Diaz-Hernandez M., Gualix J., Gomez-Villafuertes R., Miras-Portugal T.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

10

A single nucleotide polymorphism in the matrix metalloproteinase-1 promoter in breast cancer

75%

Przybylowska K., Zielinska J., Zadrozny M., Rykala J., Kolacinska A., Morawiec Z., Drzewoski J., Blasiak J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

11

Myosin VI is associated with secretory granules and is present in the nucleus in adrenal medulla chromaffin cells

63%

Majewski L., Sobczak M., Redowicz M. J.

Acta Biochimica Polonica

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2010

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tom 57

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nr 1

109-114

Myosin VI (MVI) is the only known myosin walking towards minus end of actin filaments. Here, MVI, but not myosins IB or IIB, was detected in chromaffin granules isolated from bovine medulla and found to be tightly associated with the granule apical surface. MVI also localized to secretory granules within rat pheochromocytoma PC12 cells as well as to the Golgi apparatus, endoplasmic reticulum and clathrin-coated pits. Notably, it was also found in the nucleus. RT-PCR revealed that MVI splice variants with a large insert (LI), characteristic of polarized cells, were barely detectable in PC12 cells, whereas variants with a small insert (SI) were the major isoforms. The presented data indicate that MVI in adrenal medulla cells is engaged in secretory vesicle trafficking within the cytoplasm and possibly also involved in transport within the nucleus.

12

Alpha-naphthyl and AS-BI-naphthyl esters of nucleotides, specific substrates for cytochemical localization of mammalian pancreatic RNase and 5'-exonuclease and of plant nucleotide pyrophosphatase and cyclic nucleotide phosphodiesterase

63%

Sierakowska H., Zan-Kowalczewska M., Bartkiewicz M.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 3-4

13

Methodology of Petri networks for simultaneous evaluation of the impact of different modifiers on the fluorescence of nucleotides from electron transport chain in isolated mitochondria and on the process of swelling

63%

Danylovych H., Chunikhin A., Danylovych Y., Kosterin S.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2018

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tom 99

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nr 1

14

Pathways of purine metabolism: effects of exercise and training in competitive athletes

63%

Zielinski J., Kusy K.

Trends in Sport Sciences

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2015

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tom 22

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nr 3

Introduction. The main part of skeletal muscle adenosine- 5'-triphosphate (ATP) is restored by inosine monophosphate (IMP) reamination in the purine nucleotide cycle. The intramuscular resources of IMP may be resynthesized via the quick and economical salvage pathway, in which muscle hypoxanthine (Hx) is reconverted to IMP by hypoxanthineguanine phosphoribosyl transferase (HGPRT). IMP is subsequently reutilized in the adenine nucleotide (AdN) pool. Inosine and Hx, which flow out of the skeletal muscle, represent the loss of AdN precursors. In the latter case, full restoration of resting ATP levels depends on a slow and energy-consuming de novo pathway. Plasma Hx and erythrocyte HGPRT are indirect indicators of muscle metabolism, particularly of AdN degradation, that reflect exercise- and training-induced muscle energy status. Results. Our analyses of long-term training cycles in different sports show that plasma Hx concentration and erythrocyte HGPRT activity significantly change in consecutive training phases. Both high-intensity sprint training and endurance training incorporating high-intensity exercise lead to a decrease in plasma Hx levels and an increase in erythrocyte HGPRT activity. The lowest Hx concentration and the highest HGPRT activity are observed in the competition phase characterised by low-volume and high-intensity training loads. Training cessation in the transition phase brings about a reverse phenomenon: an increase in Hx levels and a decrease in HGPRT activity. Conclusions. Low plasma purine levels indicate that the administered training adapts the athletes to high-intensity exercise (more economical AdN use, limited purine efflux from muscle into the blood). Such an adaptation is of great importance for contemporary elite athletes. Purine metabolites are more sensitive markers of training status and better performance predictors than typical biochemical and physiological indicators (e.g. blood lactate and oxygen uptake) in highly-trained athletes of different specializations and ages. The use of Hx and HGPRT for monitoring and control of the training process is worthy of consideration.

15

Cloning, sequence, expression and characterization of human beta-mannosidase

63%

Samra Z. Q., Athar M. A.

Acta Biochimica Polonica

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2008

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tom 55

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nr 3

479-490

β-mannosidase (EC 3.2.1.25, MANB) dissects the non-reducing end of N-linked mannose moieties of glycoproteins in eukaryotic cells. The human β-mannosidase gene was amplified by RT-PCR, cloned and sequenced. The DNA sequence was compared with reported human β-mannosidase DNA sequence and sixteen nucleotide differences were found. The deduced amino-acid sequence showed that seven codons coded the same amino acids and nine codons coded different amino acids with reference to nucleotide substitution positions but did not affect recombinant MANB enzyme activity. No splice mutation was observed after comparison with reported MANB DNA sequences. A 75% homology of deduced amino-acid sequence was observed with mouse, goat and bovine β-mannosidase amino-acid sequences. The cloned β-mannosidase gene was subcloned into pET22b+ and pET28a+ expression vectors to transform the BL21-codon plus cells for expression of recombinant MAN22 and MAN28 enzymes, respectively. The optimized conditions for overexpression of recombinant β-mannosidase enzyme were induction with 1 mM IPTG for 12 h at 37oC. The expressed β-mannosidase enzyme was purified to homogeneity by a combination of DEAE-ion exchange and size exclusion chromatography. The molecular mass of MAN22 and MAN28 enzymes is 97 kDa by SDS/PAGE and is confirmed by western blot analysis. The recombinant enzymes are active at 37oC and at pH 5.0 and showed activity with p-nitrophenyl-β-d-mannopyranoside and not with p-nitrophenyl-α-d-mannopyranoside. The Kmvalue of enzymes was 2.53 mM. The enzyme activity was inhibited by Zn2+, Co2+, Cu2+, Pb2+, Ag1+,iodoacetate, SDS, DMF, DMSO and ethanol. Fe3+, Ca2+mg2+, mn2+, Triton X-100 and PMSF did not inhibit the enzyme activity. Northern blot analysis showed a transcript of about 3.7 kb in all cells and tissues studied. This is the first report on the expression and characterization of recombinant human mANB enzyme.

16

Bidirectional action of extracellular ATP on intracapillary volume of isolated rat renal glomeruli

63%

Jankowski M., Szczepanska-Konkel M., Kalinowski L., Angielski S.

Journal of Physiology and Pharmacology

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2000

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tom 51

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nr 3

17

The effects of galactosamine on UTP levels in the livers of young, adult and old rats

63%

Kmiec Z., Smolenski R. T., Zych M., Mysliwski A.

Acta Biochimica Polonica

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2000

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tom 47

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nr 2

Galactosamine (GalN), a well-known hepatotoxin that depletes the cellular pool of uracil nucleotides, was previously shown to have greater impact on the inhibition of protein synthesis in hepatocytes of old rats as compared with young animals (Kmiec 1994, Ann. N.Y. Ac. Sci. 717, 216-225). In the present study we compared the effects of GalN on the nucleotide content (measured by ion-exchange HPLC) in the livers of young (4 months), adult (12 months), and old (24-26 months old) rats two hours after its intraperitoneal administration. UTP content of the livers of old control rats was significantly lower (by 28%) than that of young animals. GalN administration decreased the UTP content in the livers of young, adult and old rats by, respectively, 55%, 65% and 89%, and increased the content of UDP-sugars by 189%, 175% and 305%. The hepatic content of ATP, ADP, AMP, NAD, GTP except CTP did not differ significantly among the age groups of rats studied, and was not changed by GalN treatment. The content of CTP was significantly higher in old rats (P < 0.03) upon GalN treatment. The lower hepatic content of UTP may partially explain the increased sensitivity of hepatocytes and livers of old rats to the action of galactosamine, and possibly to other hepatotoxic compounds that decrease transcription in the liver.

18

Determination of nucleotides and its metabolic products in human saliva

63%

Kochanska B., Smolenski R. T., Knap N.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

19

Enhanced adenosine production in human endothelium by inhibition of adenosine metabolism and nucleotide precursor supply

63%

Smolenski R. T., Kalsi K. T., Gray C. C., Zych M., Kochan Z., Yacoub M. H.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

20

Changes of nucleotide content in human and rat heart during cardiac surgery and ischemia

63%

Smolenski R. T., Skladanowski A. C., Swierczynski J., Perko M., Narkiewicz M., Zydowo M. M.

Acta Biochimica Polonica

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1993

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tom 40

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nr 4

The influence of ischemia on purine nucleotide and their catabolite concentration in human myocardium was investigated during surgery of acquired and congenital heart defects. This was compared with the influence of ischemia on rat heart. Concentrations of adenine and guanine nucleotides and their catabolites were measured in the extracts of heart biopsies taken at the onset of ischemia and at the time of reperfusion. The content of myocardial ATP in human heart decreased from the initial value of 223 ± 1.1 to 14.6 ±1.5 nmol/mg protein and total adenine nucleotide pool decreased from 34.2 ± 1.8 to 27.6 ± 1.5 nmol/mg protein during the operation. Significant increases in myocardial concentrations of purine catabolites were also observed with the most prominent rise in inosine from below 0.5 at the onset of the ischemia to 3.0 ± 0.5 nmol/mg protein at the time of reperfusion. A positive correlation was demonstrated between the concentration of purine catabolites in the heart at the end of ischemia with the decrease of both ATP and the total nucleotide pool. An interesting metabolic specificity of the ischemic human heart appeared to be only a small accumulation of inosine monophospahate (IMP). The increase of IMP in the rat heart after ischemia was several-fold higher.

Ograniczanie wyników

The nucleotide sequence of 5s rRNA from bovine liver

Nucleotide probes of DNA polymerases

MicroRNA-26a-5p: multiple functions, multiple possibilities – a mini-review

A new approach to phosphorylation of nucleosides using oxyonium phosphobetaines as intermediates

Annexin VI, a novel ATP-dependent protein. Alterations of phospholipid binding properties of annexin VI by nucleotides

The effect of Arg209 to Lys mutation in mouse thymidylate synthase

Intron-containing human pre-tRNA Leu is a substrate for m5C34-methyltransferase

A single nucleotide polymorphism in the 5'untranslated region of RAD51 in breast cancer

Different responses for Ap5A and ATP in human cerebrocortical synaptic terminals

A single nucleotide polymorphism in the matrix metalloproteinase-1 promoter in breast cancer

Myosin VI is associated with secretory granules and is present in the nucleus in adrenal medulla chromaffin cells

Alpha-naphthyl and AS-BI-naphthyl esters of nucleotides, specific substrates for cytochemical localization of mammalian pancreatic RNase and 5'-exonuclease and of plant nucleotide pyrophosphatase and cyclic nucleotide phosphodiesterase