Wyniki wyszukiwania

1

Changes of nuclear DNA and NYS-protein contents during endosperm development in Zea mays cv. zlota karlowa

100%

Kuran H., Marciniak K.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2000

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tom 42

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nr 1

2

Organization of sequences homologous to tRNA in wheat nuclear genome

86%

Jarmolowski A., Szweykowska-Kulinska Z., Augustyniak J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 7-9

3

The induction of nuclear DNA breaks in human skin cells by ultraviolet a [UVA] light

86%

Brozyna A., Kwiatkowska K., Chwirot B. W.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

4

Photoaddition of psoralens to phosphatidylcholine in micelles. Cell membrane, a target for PUVA therapy

86%

Waszkowska E., Poznanski J., Zaremba Z.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

5

Karyology and amounts of nuclear DNA in Luzula L.- A genus with holokinetic chromosomes

86%

Dubas E., Kuta E., Bohanec B., Vizintin L., Przywara L.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

6

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Shikimate dehydrogenase (E.C. 1.1.1. 25 ShDH) alleles as potential markers for flowering phenology in Pinus sylvestris

72%

Prus-Glowacki W., Sukovata L., Lewandowska-Wosik A., Nowak-Bzowy R.

Dendrobiology

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2015

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tom 73

The aims of this study were 1) to determine the variability in the flowering phenology of Scots pine (Pinus sylvestris L.) clones in a seed orchard and 2) to compare the genetic structure and genetic markers (13 isozyme loci and 5 chloroplast and 3 nuclear DNA microsatellite loci) among groups of clones that are differentiated by flowering phenology. Using the timing of male inflorescence development, 57 plus trees represented by their clones in a seed orchard were classified into three phenological groups: early-, intermediate-, and late-flowering. The microsatellites showed no significant differences in the genetic structure of the analyzed phenological groups. However, the frequency of allele 2 at the shikimate dehydrogenase A locus (ShDH A 2) differed significantly between the groups of early- and late-flowering trees and between the groups of intermediate- and late-flowering trees. In addition, a significant difference in the frequencies of the genotype ShDH A 11 was observed between the intermediate- and late-flowering groups. Nei’s genetic distance indicated that the late-flowering group was the most genetically distant among the phenological groups. These results suggest that the ShDH A locus might be considered as isoenzymatic marker that differentiates these flowering groups of Scots pine clones. At several isozyme and DNA loci, the presence of private alleles in each group of pines was observed. However, these alleles cannot serve as markers of Scots pine flowering time because of their low frequencies.

7

Random forest assessment of correlation between environmental factors and genetic differentiation of populations: Case of marine mussels Mytilus

72%

Kijewski T., Zbawicka M., Strand J., Kautsky H., Kotta J., Ratsep M., Wenne R.

Oceanologia

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2019

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tom 61

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nr 1

8

Nuclear DNA degradation during development of Echinocystis lobata endosperm - programmed cell death

72%

Wojciechowska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2000

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tom 42

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nr 1

9

RAPD studies in Bromus (Poaceae) from the old and new worlds - preliminary results

72%

Joachimiak A., Sutkowska A., Mitka J.

Acta Biologica Cracoviensia. Series Botanica

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2001

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tom 43

RAPD analysis was applied to nine Bromus species (B. fasciculatus, B. arvensis, B. scoparius, B. hordeaceus, B. squarrosus, B. carinatus, B. willdenowii, B. erectus, B. inermis) belonging to four subgenera: subg. Stenobromus, subg. Bromus, subg. Ceratochloa, and subg. Festucaria. With 10 out 13 primers a considerable degree of polymorphism was detected at the interspecific level, and some bands obtained with these primers appeared to be species-specific. The RAPD band distribution and genetic relationships between different Bromus species and subgenera were analyzed. No bands common to all analyzed species were discovered, but some of the amplified DNA fragments were common to all taxa belonging to the same subgenera or even different ones. The divisions suggested by taxonomists generally were confirmed by numerical analysis of the RAPD data presented here.

10

C-banding and nuclear DNA amount in six Bromus species

72%

Joachimiak A., Kula A., Sliwinska E., Sobieszczanska A.

Acta Biologica Cracoviensia. Series Botanica

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2001

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tom 43

Conventional and C-banding chromosome studies and nuclear DN A estimations were performed on six Bromus species (B. arvensis, 2n = 2x = 14; B. hordeaceus, 2n = 4x = 28; B. carinatus, 2n = 8x = 56; B. willdenowii, 2n = 6x = 42; B. erectus, 2n = 8x = 56; and B. inermis, 2n = 8x = 56) belonging to three subgenera: subg. Bromus, subg. Ceratochloa and subg. Festucaria. Three species (B. carinatus, B. erectus and B. inermis) show different chromosome numbers within root-tip meristem. Root-tip cells of these high polyploid species (2n = 8x = 56) showed substantial DNA variability. Densitometric and flow-cytometric analyses clearly showed that many interphase nuclei are beyond the expected 2C-4C limit characteristic of cycling cells. The most distinct DNA instabilities were observed in B. carinatus, where we found especially high numerical chromosome variations and many chromosome numbers higher and lower than 2n = 8x = 56. Such quantitative DNA variability is substantially reduced beyond the meristematic part of the roots and virtually absent within leaf mesophyll cells. Estimated nuclear 2C DNA content was 11.63 pg in B. arvensis, 23.03 pg in B. hordeaceus, 22.94 pg in B. carinatus, 12.99 pg in B. willdenowii, 24.65 pg in B. erectus and 24.54 pg in B. inermis. By DNA amount, the analyzed taxa formed two distinct groups: with 2C DNA value at about 12 pg (B. arvensis and B. willdenowii), and at about 24 pg (B. hordeaceus, B. carinatus, B. erectus and B. inermis). Estimated DNA values were not linked with ploidy level. All species showed a similar, mainly telomeric heterochromatin distribution, but small amounts of intercalary and centromeric heterochromatin were also seen. The majority of analyzed species also had similar heterochromatin amounts within karyotype. The only exception was B. carinatus with 16.20% heterochromatin (calculated as percent of karyotype length). It is suggested that the observed 77% nuclear DNA difference between two closely related representatives of subg. Ceratochloa (B. carinatus and B. willdenowii) is to some extent a result of heterochromatin accumulation in B. carinatus chromosomes.

11

Flow cytometric estimation of ploidy in anisoploid sugar-beet populations

72%

Sliwinska E., Steen P.

Journal of Applied Genetics

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1995

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tom 36

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nr 2

Determination of ploidy level is a common practice in sugar-beet breeding and seed production. Lately many laboratories have replaced microscopic chromosome counting by flow cytometric estimation of the nuclear DNA content. The possibility of direct estimation of cytological composition in anisoploid populations instead of individual plant analyses was investigated. Diploid, triploid and tetraploid sugar-beet lines as well as their mixtures were tested. The results obtained show that the suggested method is fast, simple and, after a proper transformation of the computer values, could be applied in breeding and seed production of polyploid crops. Precise estimation of contamination by small admixture of seeds or plants of undesirable ploidy, however, requires individual plant rather than population analyses.

12

Mitochondrial and nuclear DNA differentiation of Picea abies populations in Poland

58%

Nowakowska J. A.

Dendrobiology

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2009

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tom 61 Supplement

The natural stands of Norway spruce in Poland are split between the southern and the northeastern parts of the country. Two so-called "spruceless" zones separate the northern spruce locations from those in the south, one "spruceless" zone is situated in Central Poland, and the other one in the Beskid Mts. Mitochondrial (STS) and nuclear (SSR) markers were used to perform the genetic identification of Norway spruce. Four different variants of haplotypes, "a", "b", "c" and "d", were found to occur in the nad1 locus of STS markers. Populations from the northern range of Picea abies distribution in Poland harboured exclusively haplotypes "c" and "d", except for the Białowieża population which had haplotypes "a" and "c". Populations from the "spruceless" zones contained four types of haplotypes whilst those from southern Poland were mostly composed of haplotype "a". High mean gene diversity was observed for both STS and SSR markers (HT = 0.529, and HT = 0.851, respectively). The total genetic differentiation of Norway spruce populations was very low (FST= 0.088). Two main groups of populations were distinguished in the dendrogram defined by Nei's genetic distances based on microsatellite markers. The distribution of the genotypes was scattered and did not show any connection with the spatial distribution of P. abies in Poland. Only the mtDNA markers were able to differentiate the northern populations of Norway spruce from the southern ones, proving the historical separation between the Baltico-Nordic and the Hercyno-Carpathian ranges of P. abies in Poland. By contrast, the microsatellite data suggested an overlap between the genotypes due to the human manipulation of Norway spruce stands in the past.

13

Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species

58%

Sliwinska E.

Folia Horticulturae

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2018

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tom 30

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nr 1

14

Association of lupin mitochondrial plasmid DNA with mitochondrial proteins

58%

Lesniewicz K., Bartkowiak S., Augustyniak H.

Biological Bulletin of Poznań

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1996

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tom 33

15

Conserved localization of restriction sites in the vicinity of tRNA gene in nuclear DNA and of tRNA pseudogene in mitochondrial DNA of wheat

58%

Karwowska U., Gozdzicka-Jozefiak A., Jakubowicz M., Szkaradkiewicz B., Augustyniak J.

Biological Bulletin of Poznań

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1994

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tom 31

16

Zastosowanie posrednich metod oznaczania poziomu ploidalnosci ziemniaka

51%

Jakuczyn H., Strzelczyk-Zyta D., Narkiewicz M.

Biuletyn Instytutu Ziemniaka

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1997

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tom 48

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nr 1

91-98

17

Genetyczna zmienność potomstwa czterech proweniencji jodły pospolitej (Abies alba MILL.) wyrażona w polimorfizmie mikrosatelitarnego jądrowego DNA (nSSRs)

51%

Pawlaczyk E. M., Chudzinska E., Bobowicz M. A.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2010

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tom 555

Przedmiotem badań było potomstwo czterech proweniencji jodły pospolitej (Abies alba MILL.) z północno-wschodniej granicy zasięgu, rosnące na powierzchni doświadczalnej w Nadleśnictwie Nawojowa. Celem badań było poznanie międzyproweniencyjnej zmienności genetycznej jodły pospolitej. Do badań wytypowano potomstwo 111 drzew matczynych (rodów) jodły z: Nadleśnictwa Białowieża, Rezerwatu Jata, Rezerwatu Kamienna Góra oraz Rezerwatu Łabowiec. Przeanalizowano zmienność 2 loci mikrosatelitarnego jądrowego DNA. Obliczono: liczbę alleli w locus (Na), liczbę efektywnych alleli (Ne), obserwowaną i oczekiwaną heterozygotyczność (Ho, He), indeks wsobności Wrightʼa (F), parametry równowagi genotypowej (FIS, FIT), współczynnik zróżnicowania międzypopulacyjnego FST, przepływ genów (Nm), oraz odległości genetyczne. Dodatkowo wykonano molekularną analizę wariancji (AMOVA), która wykazała, że populacje różnią się między sobą istotnie statystycznie. Wykryto, że 5% ogólnego wykrytego zróżnicowania występuje pomiędzy populacjami, a 95% wewnątrz populacji. Badania wykazały, że poziom zmienności genetycznej jodły jest niski w porównaniu z innymi drzewami iglastymi, pomimo że wszystkie analizowane loci były polimorficzne. Żadna populacja nie posiadała alleli unikatowych. Przepływ genów (Nm) pomiędzy populacjami był duży, a współczynnik zróżnicowania międzypopulacyjnego był niski. Najwyższą heterozygotyczność obserwowaną wykazywały populacje z Łabowca i z Kamiennej Góry, a najmniejszą populacja z Białowieży. Najwyższą heterozygotyczność oczekiwaną wykazywała populacja z Białowieży, a najmniejszą populacja z Kamiennej Góry. Wszystkie badane populacje wykazały niedobór heterozygot, a największy niedobór heterozygot zaobserwowano w potomstwie populacji z Białowieży, a najmniejszy z Kamiennej Góry. Największą odległość genetyczną zaobserwowano pomiędzy potomstwem populacji z Jaty i Białowieży, a najmniejszą pomiędzy potomstwem populacji z Łabowca i z Kamiennej Góry. Niniejsze badania wykazały, że Białowieska proweniencja różni się zdecydowanie od trzech pozostałych. Proweniencja ta, jest populacją sztuczną, założoną na początku XX wieku i powstałą z nasion niewiadomego pochodzenia. Odrębność proweniencji z Białowieży może wynikać z jej lokalizacji. Rośnie ona ok. 100 km poza granicą naturalnego zasięgu gatunku, co może ograniczać przepływ genów z innymi proweniencjami.

Ograniczanie wyników

Changes of nuclear DNA and NYS-protein contents during endosperm development in Zea mays cv. zlota karlowa

Organization of sequences homologous to tRNA in wheat nuclear genome

The induction of nuclear DNA breaks in human skin cells by ultraviolet a [UVA] light

Photoaddition of psoralens to phosphatidylcholine in micelles. Cell membrane, a target for PUVA therapy

Karyology and amounts of nuclear DNA in Luzula L.- A genus with holokinetic chromosomes

Shikimate dehydrogenase (E.C. 1.1.1. 25 ShDH) alleles as potential markers for flowering phenology in Pinus sylvestris

Random forest assessment of correlation between environmental factors and genetic differentiation of populations: Case of marine mussels Mytilus

Nuclear DNA degradation during development of Echinocystis lobata endosperm - programmed cell death

RAPD studies in Bromus (Poaceae) from the old and new worlds - preliminary results

C-banding and nuclear DNA amount in six Bromus species

Flow cytometric estimation of ploidy in anisoploid sugar-beet populations

Mitochondrial and nuclear DNA differentiation of Picea abies populations in Poland

Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species

Association of lupin mitochondrial plasmid DNA with mitochondrial proteins

Conserved localization of restriction sites in the vicinity of tRNA gene in nuclear DNA and of tRNA pseudogene in mitochondrial DNA of wheat

Zastosowanie posrednich metod oznaczania poziomu ploidalnosci ziemniaka

Genetyczna zmienność potomstwa czterech proweniencji jodły pospolitej (Abies alba MILL.) wyrażona w polimorfizmie mikrosatelitarnego jądrowego DNA (nSSRs)