Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: new agent

Sortuj według:

Ogranicz wyniki do:

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Blastocystis hominis s. l. ST6 - parasite of chickens - new zoonotic agent in Poland

100%

Lewicki A., Rozej-Bielicka W., Salamatin R.

Annals of Parasitology

2016

tom 62

nr Suppl.

Cloning and expression of a new recombinant thrombolytic and antithrombotic agent - a staphylokinase variant

67%

Kowalski M., Brown G., Bieniasz M., Oszajca K., Chabielska E., Pietras T., Szemraj Z., Makandjou-Ola E., Bartkowiak J., Szemraj J.

Acta Biochimica Polonica

2009

tom 56

nr 1

41-53

To develop a more potent antithrombin agent with thrombolytic and antiplatelet properties, a new staphylokinase (SAK) variant was constructed. The kringle 2 domain (K2) of tissue type-plasminogen activator (t-PA) containing a fibrin-specific binding site (i), the RGD sequence (Arg-Gly-Asp) for the prevention of platelet aggregation (ii) and the antithrombotic agent - hirulog (iii) was assembled to the C-terminal part of recombinant staphylokinase (r-SAK). cDNA for the hybrid protein SAK-RGD-K2-Hirul was cloned into Pichia pastoris pPIC9K yeast expression vector. The introduction of K2 t-PA, the RGD sequence and hirulog into the C-terminus of r-SAK did not alter the staphylokinase activity. We observed a higher clot lysis potency of SAK-RGD-K2-Hirul as evidenced by a faster and more profound lysis of 125I-labeled human fibrin clots. The potency of thrombin inhibition by the hirulog C-terminal part of the recombinant fusion protein was almost identical to that of r-Hir alone. These results suggest that the SAK-RGD-K2-Hirul construct can be a more potent and faster-acting thrombolytic agent with better antithrombin and antiplatelet properties compared to r-SAK and SAK-RGD-K2-Hir.

Activities of synthetic peptides against human pathogenic bacteria

67%

Bogucka K., Krolicka A., Kamysz W., Ossowski T., Lukasiak J., Lojkowska E.

Polish Journal of Microbiology

2004

tom 53

nr 1

The increasing, problem of antibiotic resistance among pathogenic bacteria requires development of new antimicrobial agents. Synthesis and experimental application of the hybrids peptides may be one of the interesting possibilities in antimicrobial treatment. The aim of the present investigation is to determinate in vitro activities of two synthetic peptide amides: cecropin-melittin hybrid peptide (CAMEL) and protegrin analogue (IB-367) against control strains and multi-resistant clinical isolates. Antimicrobial activities were measured by MIC and MBC. The tested strains were susceptible to the peptides at concentrations in the range of 1 to 32 ug ml⁻¹.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Annals of Parasitology

1 Polish Journal of Microbiology

1 Bartkowiak J.

1 Bieniasz M.

1 Bogucka K.

1 Brown G.

1 Chabielska E.

1 Kamysz W.

1 Kowalski M.

1 Krolicka A.

1 Lewicki A.

1 Lojkowska E.

1 Lukasiak J.

1 Makandjou-Ola E.

1 Ossowski T.

1 Oszajca K.

1 Pietras T.

1 Rozej-Bielicka W.

1 Salamatin R.

1 Szemraj J.

1 Szemraj Z.

1 2016

1 2009

1 2004

Wyniki wyszukiwania

Blastocystis hominis s. l. ST6 - parasite of chickens - new zoonotic agent in Poland

Cloning and expression of a new recombinant thrombolytic and antithrombotic agent - a staphylokinase variant

Activities of synthetic peptides against human pathogenic bacteria