Wyniki wyszukiwania

1

Electron probe X-ray microanalysis of cultured myogenic C2C12 cells with scanning and scanning transmission electron microscopy

100%

Tylko G., Karasinski J., Wroblewski R., Roomans G. M., Kilarski W. M.

Folia Histochemica et Cytobiologica

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2000

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tom 38

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nr 2

2

Stimulation of myogenesis by 2,2'-thiodiethanol in suboptimal tissue culture conditions

88%

Reiss K., Pietrzykowski Z., Kajstura J., Korohoda W.

Folia Histochemica et Cytobiologica

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1985

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tom 23

|

nr 3

3

Activity of mi and m-calpain in regenerating fast and slow twitch skeletal muscle

88%

Moraczewski J., Piekarska E., Zimowska M., Sobolewska M.

Acta Biochimica Polonica

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1996

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tom 43

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nr 4

Calpains — non-lysosomal intracellular calcium-activated neutral proteinases, form a family consisting of several distinct members. Two of the isoenzymes: ji (calpain I) and m (calpain II) responded differently to the injury during complete regeneration of Extensor digitorum longus (EDL) muscle and partial regeneration of Soleus muscle. In the crushed EDL the level of m-calpain on the 3rd and 7th day of regeneration was higher than in non-operated muscles, whereas the activity of this calpain in injured Soleus decreased. The level of n-calpain in EDL oscillated irregularly during regeneration whereas in Soleus of both injured and contralateral muscles its level rapidly rose. Our results support the hypothesis that m-calpain is involved in the process of fusion of myogenic cells whereas u-calpain plays a significant but indirect role in muscle regeneration.

4

Potassium currents in human myogenic cells from healthy and congenital myotonic dystrophy foetuses

75%

Nurowska E., Constanti A., Dworakowska B., Mouly V., Furling D., Lorenzon P., Pietrangelo T., Dolowy K., Ruzzier F.

Cellular and Molecular Biology Letters

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2009

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tom 14

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nr 2

336-346

The whole-cell patch clamp technique was used to record potassium currents in in vitro differentiating myoblasts isolated from healthy and myotonic dystrophy type 1 (DM1) foetuses carrying 2000 CTG repeats. The fusion of the DM1 myoblasts was reduced in comparison to that of the control cells. The dystrophic muscle cells expressed less voltage-activated K+ (delayed rectifier and non-inactivating delayed rectifier) and inward rectifier channels than the age-matched control cells. However, the resting membrane potential was not significantly different between the control and the DM1 cells. After four days in a differentiation medium, the dystrophic cells expressed the fast-inactivating transient outward K+ channels, which were not observed in healthy cells. We suggest that the low level of potassium currents measured in differentiated DM1 cells could be related to their impaired fusion.

5

Interaction of myogenic cells in the myogenesis of vertebrate skeletal muscles

75%

Kielbowna L.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

6

Myogenic cells applications in regeneration of post-infarction cardiac tissue

63%

Ciecierska A., Chodakowska K., Motyl T., Sadkowski T.

Journal of Physiology and Pharmacology

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2013

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tom 64

|

nr 4

7

The CD271 expression could be alone for establisher phenotypic marker in bone marrow derived mesenchymal stem cells

51%

Flores-Torales E., Orozco-Barocio A., Gonzalez-Ramella O., Carrasco-Yalan A., Gazarian K., Cuneo-Pareto S.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 4

8

The impairment of IGF-I-stimulated protein synthesis and activation of protein kinase B, p70S6k, MAP kinase, and p90rsk in mouse C2C12 myogenic cells exposed to high glucose and high insulin

51%

Grzelkowska-Kowalczyk K., Wieteska W.

Polish Journal of Veterinary Sciences

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2005

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tom 08

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nr 3

9

Treatment with TNF-alpha and IFN-gamma alters the activation of SER-THR protein kinases and the metabolic response to IGF-I in mouse C2C12 myogenic cells

51%

Grzelkowska-Kowalczyk K., Wietelska-Skrzeczynska W.

Cellular and Molecular Biology Letters

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2010

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tom 15

|

nr 1

13-31

The aim of this study was to compare the effects of TNF-α, IL-1β and IFN-γ on the activation of protein kinase B (PKB), p70S6k, mitogen-activated protein kinase (MAPK) and p90rsk, and on IGF-I-stimulated glucose uptake and protein synthesis in mouse C2C12 myotubes. 100 nmol/l IGF-I stimulated glucose uptake in C2C12 myotubes by 198.1% and 10 ng/ml TNF-α abolished this effect. Glucose uptake in cells differentiated in the presence of 10 ng/ml IFN-γ increased by 167.2% but did not undergo significant further modification upon the addition of IGF-I. IGF-I increased the rate of protein synthesis by 249.8%. Neither TNF-α nor IFN-γ influenced basal protein synthesis, but both cytokines prevented the IGF-I effect. 10 ng/ml IL-1β did not modify either the basal or IGF-I-dependent glucose uptake and protein synthesis. With the exception of TNF-α causing an 18% decrease in the level of PKB protein, the cellular levels of PKB, p70S6k, p42MAPK, p44MAPK and p90rsk were not affected by the cytokines. IGF-I caused the phosphorylation of PKB (an approximate 8-fold increase above the basal value after 40 min of IGF-I treatment), p42MAPK (a 2.81-fold increase after 50 min), and the activation of p70S6k and p90rsk, manifesting as gel mobility retardation. In cells differentiated in the presence of TNF-α or IFN-γ, this IGF-I-mediated PKB and p70S6k phosphorylation was significantly diminished, and the increase in p42MAPK and p90rsk phosphorylation was prevented. The basal p42MAPK phosphorylation in C2C12 cells treated with IFN-γ was high and comparable with the activation of this kinase by IGF-I. Pretreatment of myogenic cells with IL-1β did not modify the IGF-I-stimulated phosphorylation of PKB, p70S6k, p42MAPK and p90rsk. In conclusion: i) TNF-α and IFN-γ, but not IL-1β, if present in the extracellular environment during C2C12 myoblast differentiation, prevent the stimulatory action of IGF-I on protein synthesis. ii) TNF-α- and IFN-γ-induced IGF-I resistance of protein synthesis could be associated with the decreased phosphorylation of PKB and p70S6k. iii) The activation of glucose uptake in C2C12 myogenic cells treated with IFN-γ is PKB independent. iv) The similar effects of TNF-α and IFN-γ on the signalling and action of IGF-I on protein synthesis in myogenic cells could suggest the involvement of both of these cytokines in protein loss in skeletal muscle.

10

Exposure to TNF-alpha but not IL-1beta impairs insulin-dependent phosphorylation of protein kinase B and p70S6k in mouse C2C12 myogenic cells

51%

Grzelkowska-Kowalczyk K., Wieteska-Skrzeczynska W.

Polish Journal of Veterinary Sciences

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2006

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tom 09

|

nr 1

Tumor necrosis factor (TNF)-a is a proinflammatory cytokine considered to play an important role in muscle catabolism, but little is known about the mechanisms of its action. The aim of the present study was therefore to examine the effect of TNF-α pretreatment on glucose uptake and protein synthesis as well as the cellular content and phosphorylation of protein kinase B (PKB), p70S6k, Mitogen Activated Protein (MAP) kinase and p90 rsk in mouse C2C12 myotubes stimulated with insulin. To determine whether interleukin (IL)-lß might be involved in the catabolic action of TNF-α, the effects of IL-lß were also tested. Experiments were performed on mouse C2C12 myoblasts subjected to differentiation in the presence of increasing concentrations of TNF-α (0.1-100 ng/ml) or IL-lß (5-50 ng/ml) for 5 or 6 days. Insulin (100 nmol/1) markedly stimulated glucose uptake in C2C12 myotubes (202.6% of control). This effect was profoundly attenuated by pretreatment with TNF-a at a concentration of 1 ng/ml (122.2% of control) and completely abolished by higher cytokine concentrations. Pretreatment of cells with TNF-a at a concentration of 1 ng/ml was also effective in diminishing the effect of insulin on protein synthesis, whereas higher cytokine concentrations prevented hormonal stimulation of protein synthesis in C2C12 myotubes. Pretreatment with TNF-a caused a significant decrease in PKB protein content. Insulin-mediated activation of protein kinase B was significantly diminished in cells differentiated in the presence of TNF-a. Treatment of C2C12 cells with insulin led to the gel mobility retardation of p70S6k indicating its phosphorylation and activation. In cells differentiated in the presence of TNF-a an approximately 2-fold decrease of insulin-mediated p70s6k phosphorylation was noted. Six-day differentiation of myogenic cells in the presence of TNF-α did not affect the protein content of p42MAPK, p44MAPK, p90rsk and phosphorylation of p42MAPK. Neither glucose uptake nor protein synthesis stimulated by insulin were affected significantly by pretreatment with IL-lß. Preincubation of myogenic cells with IL-lß did not modify either the protein content of PKB and p70S6k or the insulin-stimulated phosphorylation of these kinases. In conclusion: i) high concentrations of TNF-α, but not IL-1 ß, present in the extracellular environment during myoblast differentiation prevent the stimulatory action of insulin on glucose uptake and protein synthesis; ii) insulin resistance induced by TNF-α in C2C12 myogenic cells could be associated with the decreased insulin-mediated phosphorylation of PKB and p70S6k, but not with the basal phosphorylation of p42MAPK.

11

Syndekan-4 distribution during the differentiation of satellite cells isolated from muscle extensor digitorum longus and soleus treated with phorbol ester and calphostin C

51%

Brzoska E., Grabowska I., Moraczewski J.

Cellular and Molecular Biology Letters

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2002

|

tom 07

|

nr Suppl.

12

Myonuclear accretion - a brief review

51%

Mozdziak P. E., Giamario C., Petitte J. N.

Animal Science Papers and Reports. Supplement

|

2003

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tom 21

|

nr 1

Ograniczanie wyników

Electron probe X-ray microanalysis of cultured myogenic C2C12 cells with scanning and scanning transmission electron microscopy

Stimulation of myogenesis by 2,2'-thiodiethanol in suboptimal tissue culture conditions

Activity of mi and m-calpain in regenerating fast and slow twitch skeletal muscle

Potassium currents in human myogenic cells from healthy and congenital myotonic dystrophy foetuses

Interaction of myogenic cells in the myogenesis of vertebrate skeletal muscles

Myogenic cells applications in regeneration of post-infarction cardiac tissue

The CD271 expression could be alone for establisher phenotypic marker in bone marrow derived mesenchymal stem cells

The impairment of IGF-I-stimulated protein synthesis and activation of protein kinase B, p70S6k, MAP kinase, and p90rsk in mouse C2C12 myogenic cells exposed to high glucose and high insulin

Treatment with TNF-alpha and IFN-gamma alters the activation of SER-THR protein kinases and the metabolic response to IGF-I in mouse C2C12 myogenic cells

Exposure to TNF-alpha but not IL-1beta impairs insulin-dependent phosphorylation of protein kinase B and p70S6k in mouse C2C12 myogenic cells

Syndekan-4 distribution during the differentiation of satellite cells isolated from muscle extensor digitorum longus and soleus treated with phorbol ester and calphostin C

Myonuclear accretion - a brief review