Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 11

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  model system
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
 Model systems such as black lipid membranes or conventional uni- or multilamellar liposomes are commonly used to study membrane properties and structure. However, the construction and dimensions of these models excluded their direct optical microscopic observation. Since the introduction of the simple method of liposome electroformation in alternating electric field giant unilamellar vesicles (GUVs) have become an important model imitating biological membranes. Due to the average diameter of GUVs reaching up to 100 µm, they can be easily observed under a fluorescent or confocal microscope provided that the appropriate fluorescent probe was incorporated into the lipid phase during vesicle formation. GUVs can be formed from different lipid mixtures and they are stable in a wide range of physical conditions such as pH, pressure or temperature. This mini-review presents information about the methods of GUV production and their usage. Particularly, the use of GUVs in studying lipid phase separation and the appearance and behavior of lipid domains (rafts) in membranes is discussed but also other examples of GUVs use in membrane research are given. The experience of the authors in setting up the GUV-forming equipment and production of GUVs is also presented.
The influence of food gums (guar, xanthan, arabic) and carboxymethylcellulose (CMC) on bitterness and astringency of caffeine and tannic acid has been studied. The study was performed in critical concentrations (c*) for particular hydrocolloids as well as for values above and below c*. The ability of hydrocolloids to reduce the astringency and bitterness was evaluated using the method of taste indicator and expressed as a percentage of unreduced sensation. The results indicated that the ability of hydrocolloids to mask the astringency and bitterness was differential and depended both on the concentration and the type of the hydrocolloids used. CMC indicated the highest ability to mask bitterness and astringency among the hydrocolloids. It was found that the ability of these polymers to reduce the astringency was increasing above the c* concentrations.
Mitochondria are key regulators of energy metabolism, redox balance, calcium homeostasis, and programmed cell death. In the past, we characterized mitochondria acting as targets of both caspase-dependent and caspase- independent death signalling triggered by increased oxidative stress and as executioners of programmed death signalling in neurons. For example, we identified mitochondrial damage in caspase‑independent neuronal death after cerebral ischemia in vivo, and in oxidative cell death, i.e., ferroptosis in vitro. Protective intervention against oxidative damage further confirmed the conclusion that mitochondria represent the “point of no return” in caspase‑independent paradigms of programmed cell death. Further, we found more recently that mitochondri al-targeted alpha-synuclein caused severe mitochondrial toxicity and caspase-dependent cell death in human dopaminergic neurons, a model system relevant to Parkinson’s disease. In different model systems of neuronal death, neuroprotective interference with mitochondrial pathways of programmed cell death was frequently attributed to metabolic switches, i.e., reduced mitochondrial respiration and increased glycolytic activity. Accordingly, targeting metabolic switches may serve as a general strategy for mitochondrial protection and, thereby, neuroprotection, but may also affect mechanisms of neuroinflammation involving activation of microglia. The understanding of the underlying mechanism of such metabolic protection may reveal novel therapeutic targets in neural diseases featuring mitochondrial impairments and neuroinflammation.
W pracy określano podatność aminokwasów występujących w postaci jonu obojnaczego i postaci kationu na reakcje z produktami utleniania tłuszczu. Prowadzono badania modelowe w emulsjach estrów metylowych kwasów tłuszczowych: dokozaheksaenowego, linolowego, oleinowego i stearynowego.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.