Wyniki wyszukiwania

1

Studies on metabolic activity of planktonic bacteria isolated from coastal Lake Lebsko

100%

Mudryk Z.

Polish Journal of Environmental Studies

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1997

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tom 06

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nr 4

Metabolic activity of heterotrophic bacteria isolated from coastal lake Łebsko was determined using the respirometric method. It has been shown that planktonie bacteria oxidized organic compounds with various intensity. Caseine hydrolyzate was the most intensively oxidized respiratory substrate, whereas cellobiose was oxidized the least intensively. In the studied lake, oxygen was taken up most actively by bacteria of the Flavobacterium-Cytophaga group and the genus Pseudomonas in their respiratory processes. The level of metabolic activity of bacteria from different parts of the lake was shown to differ. The oxygen uptake of bacterial mixtures was, as a rule, lower than the sum of oxygen uptake of single strains.

2

Assessment of metabolic activity of single and mixed microorganism population assigned for potato wastewater biodegradation

88%

Lasik M., Nowak J., Kent C. A., Czarnecki Z.

Polish Journal of Environmental Studies

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2002

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tom 11

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nr 6

An attempt was made to characterise thermophilic microflora isolated in an industrial plant utilising food industry waste. Initial estimation of environmental requirements of a mixed microorganism population was performed using the method measuring changes in electrical impedance by means of the BacTrac 4100 Automatic Microorganisms Growth Analyzer. Control of the dynamics of microorganism growth on broth media supplemented with sources of carbon, nitrogen and phosphorus and at various pH allowed initial assessment of their environmental requirements. The course of alterations in electrical impedance during culturing of both single isolates and mixed microorganism populations can be described by means of Gompertz’s curve characteristic for bacteria growth with a very high correlation coefficient. The mixed microorganism population and isolates obtained from it were also characterised from the point of view of their biodegradation potentials of wastewater obtained in the potato processing industry. Biodegradation processes carried out in conditions of shake flask cultures at a temperature of 55oC showed higher reducing potentials of chemical oxygen demand (COD) of a mixed bacteria culture. Single isolates were characterised by a distinctly inferior metabolic activity and lower biodegradation potentials of “ hot” potato wastewater.

3

Role of ATP, dinucleoside polyphosphates and adenosine receptors in renal function

88%

Angielski S.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

4

Cationic porphyrin-mediated photodynamic inactivation of Candida biofilms and the effect of miconazole

75%

Davies A., Gebremedhin S., Yee M., Padilla R. J., Duzgunes N., Konopka K., Dorocka-Bobkowska B.

Journal of Physiology and Pharmacology

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2016

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tom 67

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nr 5

5

Ultrastructural studies on diurnal variations in mitochondria in various regions of the hepatic lobule

75%

Cieciura L., Okroj W., Krakowski G., Godlewski W.

Folia Histochemica et Cytobiologica

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1985

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tom 23

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nr 3

6

Does the near-infrared radiation (NIR) destabilize the DNA molecule?

75%

Szymborska-Malek K., Komorowska M.

Current Topics in Biophysics. Supplement

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2011

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tom 34

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nr A: Posters

7

Development and metabolic activity of bacteria isolated from polyhumic Stegwica Lake

75%

Donderski W., Walczak M., Wodkowska A.

Polish Journal of Environmental Studies

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1997

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tom 06

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nr 5

This study concerns developmental intensity and metabolic activity of heterotrophic bacteria isolated from water of the littoral and pelagial zones of the polyhumic lake Stęgwica. The experiments were carried out with humic substances and [3H]-glucose in media with pH 6.0 and 8.0. It was found that the development and metabolic activity of most strains in the presence of humic substances were higher in the medium with pH 8.0 than in that with pH 6.0. It was found that humic substances in acid medium had an inhibitory effect on the development and metabolic activity of 90% of the strains of bacteria isolated from littoral zone and of 50% of the strains isolated from the pelagial zone.

8

Can the callus tissue metabolic activity be an indicator of its ability for in vitro differentiation?

75%

Zur I., Koscielniak J.

Acta Physiologiae Plantarum

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1997

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tom 19

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nr 2

9

Interactions of antitumour triazoloacridinones with DNA

75%

Koba M., Konopa J.

Acta Biochimica Polonica

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2007

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tom 54

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nr 2

Triazoloacridinones (TA) are a new group of potent antitumor compounds, from which the most active derivative, C-1305, has been selected for extended preclinical trials. This study investigated the mechanism of TA binding to DNA. Initially, for selected six TA derivatives differing in chemical structures as well as cytotoxicity and antitumor activity, the capability of noncovalent DNA binding was analyzed. We showed that all triazoloacridinones studied stabilized the DNA duplex at a low-concentration buffer but not at a salt concentration corresponding to that in cells. DNA viscometric studies suggested that intercalation to DNA did not play a major role in the mechanism of the cytotoxic action of TA. Studies involving cultured cells revealed that triazoloacridinone C-1305 after previous metabolic activation induced the formation of interstrand crosslinks in DNA of some tumor and fibroblast cells in a dose dependent manner. However, the detection of crosslink formation was possible only when the activity of topoisomerase II in cells was lowered. Furthermore, it was impossible to validate the relevance of the ability to crosslink DNA to biological activity of TA derivatives.

10

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Fluorescent microscopy and gas chromatography to assess the viability and metabolic activity of Propionibacterium sp. strains

63%

Warminsko-Radyko I., Sielawa H., Miks-Krajnik M.

Polish Journal of Food and Nutrition Sciences

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2010

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tom 60

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nr 4

The aim of the study was to assess usefulness of the LIVE/DEAD fluorescent staining method and gas chromatography to monitor the viability and metabolic activity of Propionibacterium strains in long-term cultures in milk. The effect of 4% NaCl addition and a temperature of 10°C on the growth of Propionibacterium freudenreichii ssp. freudenreichii 111, 109C, 108 strains was studied for 28 days. Bacterial cells were assessed in cultures by microscopic and plate counting methods in regular intervals. The cultures were additionally determined for the content of volatile fatty acids: C2 to C7. The total cell counts of all strains in cultures assessed by the microscopic method were noticed to be 1 to 5 logarithmic cycles higher in comparison to those determined with the plate counting method. In following days and weeks of culture, increasing discrepancies were observed between the results obtained using microscopic and plate methods. Both methods revealed similar trends in the viability of strains under control conditions and a little impact of NaCl addition on cell growth and decrease. The cultures run at a temperature of 10°C exhibited different course of growth and decline of the number of monitored populations depending on strain and method applied. Individual strains possessed different acid formation activity. From the beginning of incubation, the highest concentrations were reported for propionic and acetic acids, whereas the other acids in number from 4 to 6 appeared subsequently. The temperature of 10°C inhibited acids formation by all strains, whereas 4% addition of NaCl stimulated the acid-forming activity and during incubation under those conditions the contents of volatile acids were recorded to be the highest.

11

Stimulation effect of Microcystis aeruginosa MKR 0105 on the metabolic activity and development of willow (Salix viminalis L.) plants

63%

Grzesik M., Romanowska-Duda Z., Pszczolkowska A., Pszczolkowski W.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

12

In vitro influence of ciprofloxacin on selected functions of rainbow trout (Oncorhynchus mykiss) leucocytes

63%

Sieroslawska A., Siwicki A. K.

Polish Journal of Veterinary Sciences

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2003

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tom 06

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nr 3 Suppl.

13

Boar variability affects sperm metabolism activity in liquid stored semen at 5 degrees Celsius

63%

Dziekonska A., Strzezek J.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 1

Metabolic activity of boar spermatozoa, liquid stored for three days at 5℃, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5℃ may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.

14

New method for growth investigation of metabolically active microorganisms in mixed cultures

63%

Kitzman P.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2006

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tom 09

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nr 4

15

Massage-induced morphological changes of dense connective tissue in rat’s tendon

63%

Kassolik K., Andrzejewski W., Dziegiel P., Jelen M., Fulawka L., Brzozowski M., Kurpas D., Gworys B., Podhorska-Okolow M.

Folia Histochemica et Cytobiologica

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2013

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tom 51

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nr 1

16

Detection of bacterial etiology using bioluminescence method on cup lids due to the change in their frequency of use – short communication

63%

Biegalski J.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2020

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tom 23

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nr 2

17

Effects of dietary gamma-cyclodextrin on voluntary activity and muscle strength in mice

63%

Wupper S., Fischer A., Luersen K., Ipharraguerre I. R., Chikamoto K., Furune T., Ishida Y., Terao K., Rimbach G.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 3

18

Adaptation dependent metabolic activity of bacteria Thiobacillus ferrooxidans

63%

Cwalina B., Dzierzewicz Z.

Acta Biologica Cracoviensia. Series Botanica

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1991

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tom 33

19

The influence of resveratrol and quercetin on prooxidative effects of metabolic activation of benzo[a]pyrene in vitro

63%

Gawlik M., Gawlik M. B., Brandys J.

Acta Toxicologica

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2007

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tom 15

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nr 2

157-163

20

Response of birch and alder root endophytes as well as rhizosphere and bulk soil microorganisms to heavy metal pollution

63%

Zloch M., Kowalkowski T., Szymanska S., Hrynkiewicz K.

Polish Journal of Ecology

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2014

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tom 62

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nr 1

Research on soil microorganisms associated with plant roots (endophytes) and their outside habitats (rhizosphere, soil) under exposure to high concentrations of heavy metals in the environment may contribute to an increased knowledge about the effects of unfavorable pollutants in the natural ecosystem and improve the efficiency of remediation processes of degraded soils. The main goal of our study was to evaluate properties of microbial populations inhabiting different but spatially closed habitats: interior of the tree roots – endophytes (Betula pendula L. and Alnus glutinosa L.), rhizosphere and soil not influenced by plant roots at two heavy metal polluted areas in southern Poland where the content of cadmium, lead and zinc ranged 9.2–14.9, 26.2–75.1 and 1080–1559 mg∙kg–1 of dried soil, respectively. In our experiment, we analyzed their density as well as determined the total metabolic activity and ability for utilization of different carbon sources. The density of endophytes (5.76–7.16 log10 c.f.u. × g dw–1) was higher than the density of bacteria inhabiting outside habitats – the rhizosphere and soil (5.43–5.91 and 5.08–5.57 log10 c.f.u. × g dw–1, respectively) and was not affected by heavy metal content. However, the total metabolic activity of endophytic populations was strongly influenced by the physicochemical properties of the soil and was significantly lower at more contaminated site. In general, the metabolic activity of endophytes was ~15 times lower than the activity of rhizobacteria and at the same level or lower than the activity of soil microorganisms. Discriminant analysis revealed a higher utilization of α-Cyclodextrin and D-Xylose by microorganisms isolated from more polluted test site. The use of an artificial neural network (ANN) followed by principal component analysis (PCA) allowed the distinguishing of preferences in the utilization of carbon sources by microorganisms due to tree species but not the zone of isolation – endophytes, rhizosphere, soil. Our study revealed no significant impact of the metal concentrations present in the soil on the density of microorganisms (bacteria and fungi) inhabiting three analyzed zones (interior of the roots, rhziosphere, soil); however, their total metabolic activity was decreased in more contaminated site. Since the density of endophytes was higher compared to the number of bacteria present in the outside habitats (rhizosphere and soil), and their total metabolic activity was in general lower, we suggest a high specialization of microorganisms associated with the cellular spaces of plant roots compared to other microbial populations.

Ograniczanie wyników

Studies on metabolic activity of planktonic bacteria isolated from coastal Lake Lebsko