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1

New tools for the manipulation of microspore gene expression

100%
Honys D., Oh S.-A., Solcova B., Johnson J. A., Boudova R., Douda J., Twell D.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2005
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tom 47
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nr 1
2

Multifactor analysis of time consumption in manipulation and cutting stacked wood into length

86%
Szewczyk G., Sowa J. M., Kulak D., Stanczykiewicz A.
Acta Scientiarum Polonorum. Silvarum Colendarum Ratio et Industria Lignaria
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2012
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tom 11
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nr 2
Cutting stacked wood into length by means of a petrol chainsaw is a typical technical and technological wood harvesting solution. Due to the fact that wood harvesting is most commonly performed with the use of Tree-Length-System (TLS) or Long-Length-System (LLS), cutting into length in Poland is performed either after the first stage of skidding stems or logs to the skidding route or after the second stage of transportation, that is at the depot. Research was carried out in depots, during early and late thinnings of pine, fir, spruce and beech stands by means of manipulation and cutting into lengths using petrol chainsaws (Table 1). The aim of the study was to specify time consumption on the basis of the relativeness of EST = f (category of harvesting, roller’s length, chosen elements of the working day structure). Continuous time-motion analysis was carried out, according to the classification presented in Table 2. After completing harvesting, the produced material was received. Figure 1 presents the percentage of work activities observed in operational time. Performing cutting into length activities at a discussed working site resulted in high time percentage in case of T12 and T22 times. Table 3 presents the average values of time consumption correlated in terms of soft and hard wood species in the categories of early and late thinning stands. Some statistically significant differences in labour consumption levels have been demonstrated in case of specific stands and harvesting technologies. One multiple regression equation has been estimated – for early and late thinning. The regression model has been expressed by a formula (1). The equation parameters have been compiled in Table 4. Research results point to the relation between time consumption, the specific elements of the time structure of a working day, and the length of produced rollers expressing variable volume of produced rollers.
3

Araceae embryos as tools for ploidy manipulation and somatic fusion

72%
Eeckhaut T., Werbrouck S., Wielowska J., van Huylenbroeck J.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2005
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tom 47
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nr 1
4

Manipulations of poultry embryonic cells

72%
Varkonyi E., Hidas A., Szalay I.
Zeszyty Naukowe. Przegląd Hodowlany
|
1997
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tom 31
5

Pleiotropic effect of flavonoid biosynthesis manipulation in transgenic potato plants

72%
Lukaszewicz M., Szopa J.
Acta Physiologiae Plantarum
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2005
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tom 27
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nr 2
Three approaches were successfully used to manipulate content of flavonoids in transgenic plants. Overexpressing either the adaptor 14-3-3 protein or genes coding the key enzymes of the flavonoid biosynthesis pathway resulted in a significant increase in the compound content in potato tuber epidermis. The opposite effect was observed in transgenic plants in which these proteins were repressed; this strongly supports the view that the gene construct deiermines transgenic plant feaiures. The most effective construct was, however, the one containing single dihydroflavonol reductase (DFR) gene in sense orientation. In all cases the increase in flavonoid content resulted in the expected enhancement of the antioxidant capacity of tuber extract. At the biochemical level a decrease in the starch content in transgenic plant overexpressing proteins regulating flavonoid biosynthesis was detected. In the case of glucosyl transferase (GT) gene overexpression, the content of phenolic compounds remained at the control level, however, the antioxidant capacity of tuber extracts significantly decreased. The GT plants grew faster glucosylation of flavonoids rather than their quantity which determines transgenic plant features.
6

Przezywanie zarodkow meskich i zenskich poddanych manipulacji in vitro

58%
Sygula J.
Biuletyn Informacyjny. Instytut Zootechniki
|
1997
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tom 35
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nr 4
29-33
7
Content available remote

Gastrointestinal tract and digestion in the young ruminant: ontogenesis, adaptations, consequences and manipulations

58%
Guilloteau P., Zabielski R., Blum J. W.
Journal of Physiology and Pharmacology. Supplement
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2009
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tom 60
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nr 3
37-46
8

Przemoc i manipulacja w dzialaniach wobec konkurentow

58%
Kardaszewski J.
Marketing i Rynek
|
2001
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tom 08
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nr 02
15-19
9

In vitro manipulation of cucumber [Cucumis sativus L.] pollen and microspores: isolation procedures, viability tests, germination, maturation

58%
Vizintin L., Bohanec B.
Acta Biologica Cracoviensia. Series Botanica
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2004
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tom 46
The distribution of plastids at the time of microspore and pollen grain development in Gagea lutea (L.) Ker.-Gaw. was analyzed using electron microscopy. It was shown that plastids are not transmitted to the forming generative cell of this species during microspore division. At the vacuolate microspore stage, preceding division, the microspore nucleus takes an acentric position and the plastids gather at the opposite side of the cell. In the highly polarized microspore at prophase of mitosis, all plastids are aggregated at one side of the nucleus, whereas mitochondria are dispersed throughout the cytoplasm. Numerous profiles of endoplasmic reticulum (ER) are present between the clustered plastids. Some of the ER profiles are attached by their ends to the outer membrane of plastid envelopes and join the distant plastids. The outer membrane of the microspore plastids may form long and thin evaginations contacting with other plastids. Microtubules are visible in plastid aggregations occasionally. In dividing microspores, long ER cisterns surround the area of the mitotic spindle and separate it from the region containing plastids. There are no plastids in the young generative cell: all plastids remain clustered in the region of the microspore that now forms the vegetative cell of the bicellular pollen grain. Later the connections between plastids and ER cisterns gradually disappear and plastids disperse in the cytoplasm of the whole vegetative cell. The results of our study are not sufficient to define the mechanism causing selective aggregation of plastids at the vegetative pole of the Gagea microspore, nor to say whether the microtubular cytoskeleton plays a role. However, the participation of ER in these processes, at least in holding the special arrangement of microspore plastids, seems certain.
10
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Content available

Multiple strategies of digenean trematodes to complete their life cycles

51%
Niewiadomska K., Pojmanska T.
Wiadomości Parazytologiczne
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2011
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tom 57
|
nr 4
11

Some approaches to the experimental manipulation of reproductive cells and protoplasts in flowering plants

44%
Zhou C., Yang H. Y.
Acta Biologica Cracoviensia. Series Botanica
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2000
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tom 42
|
nr 1
One recent advance in plant embryology is the experimental manipulation of various reproductive cells and their protoplasts under in vitro conditions. These experimental means may be helpful for understanding the developmental biology of sexual plant reproduction, on one hand, and developing novel methods in biotechnology, on the other. This article reviews a series of our works in this field. The article includes manipulation of pollen protoplasts, de-exined pollen, male gametoplasts and female gametoplasts. Each section starts with isolation of the cells/protoplasts and is followed by further manipulations such as culture, fusion, gene transfer, and also some cell biology studies based on and related to these manipulations.
12

Socjotechnika narzedziem hakera

37%
Chmielewski J. M.
ABC Jakości
|
2005
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nr 4
42-46
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