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The fraction of sterols acetates from fruits and leaves of P. coccínea have been isolated in a typical way from a petroleum ether extract. Besides, by means of the weight method the total amounts of sterols were determined in fruits and leaves (148.2 mg and 75.36 mg per 100 g, respectively), ß-sitosterol, cholesterol, stigmasterol and campesteryl were identified in both fractions by GC, GC/MS and TLC techniques, ß-sitosterol was the predominant sterol component in analysed raw materials obtained from P. coccínea.
Phenolic acids from leaves and roots ofJovibarba sobolifera (Sims.) Opiz were analysed by means of the two-dimensional thin layer chromatography and by the RP-HPLC method. Twelve phenolic acids were identified by 2D-TLC: chlorogenic, gallic, protocatechuic, caffeic, p-coumaric, p-hydroxy- benzoic, p-hydroxyphenylacetic, vanillic, syringic, ferulic, synapic and a-rezorcylic. The RP-HPLC enabled describing the contents often major phenolic acids in the examined material.
The contents of phenolic acids in the leaf extracts of ten Aquilegia L. species (A. vulgaris L., A. alpina L.,/4- atrata Koch., A. canadensis l.,A. caerulea James, A. flabellata Sieb, et Zucc., A. hybrida Scott- Elliot, A. olympica Boiss., A. pyreneica D.C., A. vitoleili L.) were determined by the spectroscopic methods and expressed as caffeic acid equivalent. The obtained results ranged from 0.31% to 1.13%. The qualitative analysis was performed by 1D- and 2D-TLC chromatography against the standards. In all studied samples the following acids were found: p-hydroxybenzoic, vanillic, protocatechuic, p-coumaric, caffeic, ferulic, and chlorogenic. Moreover, in some extracts sinapic, gallic, a- and y-resorcylic acids were detected.
The paper reports results of a study on the variability of six morphological features of leaves of Chamaedaphne calyculata (L.) Moench, collected at nine sites in Poland and two abroad. The size of the leaves was found to be strongly dependent on the habitat conditions and was the parameter determining the division into ecophenotypic groups. A linear relation was observed between the length and the width of the leaves.
The effect of methyl jasmonate (MJ) on the water-soluble protein pattern of Ricinus communis leaves was analyzed. Several dynamic changes occurred after a period of 24 and 48h including six proteins (Mr 13,000, 15,000, 16,000, 27,000, 29,000 and 60,000) whose levels increased by 48h and seven others (Mr 11,000, 18,000, 20,000 30,000, 37,000, 40,000 and 58,000) whose levels decreased. Four proteins (Mr 24,000, 34,000, 64,000 and 66,000) were induced after 24h of treatment, but returned to control levels by 48h. On the other hand, the levels of three proteins (Mr 74,000, 84,000 and 88,000) decreased after 24h, but returned to control levels after 48h. One of the proteins that accumulated after 48 h had the 13 first residues sequenced. This polypeptide named MJRC-15, was identical to the C-terminal sequence of Rubisco-large chain polypeptide (position 337–350) from tobacco chloroplast. Western-blot analysis using polyclonal antibodies against Rubisco supports the hypothesis that MJRC-15 is a degradation product of Rubisco.
GC MS methods were used to analyse sterol components of leaves and roots of Jovibarba sobolifera (Sims.) Opiz. ß-sitosterol, campesterol, 24-ethylcholestanol, 5ot-ergostan-3a-ol, -y-ergosteryl and 5a-stigmast-7-en-3a-ol have been identified in both fractions, ß-sitosterol and campesterol have been the predominant sterol components in the analysed leaves, whereas ß-sitosterol and 24-ethylcholestanol have been the main sterol components in the roots. Besides, by means of the weight method a total amount of sterols has been determined: 0.04% in leaves and 0.02% in roots.
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