Wyniki wyszukiwania

1

Phosphomannose isomerase gene for selection in lettuce [Lactuca sativa L.] transformation

100%

Briza J., Ruzickova N., Niedermeierova H., Dusbabkova J., Vlasak J.

Acta Biochimica Polonica

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2010

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tom 57

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nr 1

63-68

A positive selection system using phosphomannose isomerase was employed for Agrobacterium tumefaciens mediated transformation of lettuce (Lactuca sativa L. var. 'Achát'). It was shown that the mannose-based selection system works very well with the lettuce genotype used, reaching up to 25 % transformation efficiency on the medium with 20 g/L mannose and 20 g/L sucrose. The best transformation efficacy with the commonly-used kanamycin at 100 mg/L as a selection agent was 21 %. Southern blot analyses of thirteen chosen mannose-resistant regenerants revealed that some of them have clonal origin, about one-half harbour a single T-DNA copy and one plant contains an incomplete T-DNA segment with only the left part of T-DNA with the pmi gene present in the genomic DNA. The following Northern analysis showed transcriptional activity of the introduced pmi gene in all plants analysed with very high differences in the level of pmi specific mRNA. The results demonstrate that both mannose and kanamycin provide comparable transformation efficiencies in our lettuce genotype. An alternative selection method with mannose as a selection agent is now; available for lettuce transgenosis.

2

Genetic translocation of resistance determinants in Staphylococcus aureus

100%

Mlynarczyk A., Mlynarczyk G., Grzesik A., Sawicka-Grzelak A., Lipnicki D., Dworczynski A., Osowiecki H.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1987

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tom 35

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nr 4-6

3

Filter paper inhibits in vitro protocorm-like body formation in hybrid Cymbidium and reduces synseed germination, but buffers the negative impact of antibiotics

100%

Teixeira Da Silva J. A.

Biological Letters

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2014

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tom 51

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nr 1

4

Differentiation of adventitious buds from Capsicum annuum L. hypocotyls after co-culture with Agrobacterium tumefaciens

100%

Delis M., Garbaczewska G., Niemirowicz-Szczytt K.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 1

In vitro organogenesis in hypocotyl explants of the pepper cultivar ‘Bryza’ was induced on MS medium containing 5 mg/l 6-benzyloaminopurine (BAP) and 1 mg/l indole-3-acetic acid (IAA). The hypocotyl explants were then inoculated with Agrobacterium tumefaciens LBA4404(pBI121). After 2 days of culture the first cell divisions were observed in the epidermis and cortex. After 6-7 days, numerous adventitious bud primordia appeared in 58.4% of the explants. In further stages of culture, buds developed into shoots in 8.4% of the explants. Histological analysis revealed hypertrophy and the presence of necrotic cells in the cortex. Necrotic changes were also observed in the vascular bundles. It is likely that culture on a selective medium containing kanamycin and co-culture with Agrobacterium tumefaciens strongly affected the organization of the hypocotyl meristematic tissue, and in consequence brought about necrosis and isolation of the adventitious buds from the vascular bundles.

5

Fertile transgenic Nicotiana benthamiana plants carrying capsid protein cDNA of plum pox virus

100%

Wypijewski K., Korcz-Szatkowska H., Cajza M., Augustyniak J.

Journal of Applied Genetics

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1996

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tom 37A

6

Effects of the secondary metabolite producing Pseudomonas fluorescens CHA0 on soil Protozoa and bacteria

80%

Winding A., Oberender J.

Acta Protozoologica

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2012

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tom 51

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nr 3

7

Transformation of diploid potato with an Agrobacterium tumefaciens binary vector system: I. Methodological approach

80%

Borkowska M., Kleczkowski K., Klos B., Jakubiec J., Wielgat B.

Acta Physiologiae Plantarum

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1994

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tom 16

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nr 3

8

Antybiotykoterapia u świń cz.4. Antybiotyki aminoglikozydowe

61%

Pomorska-Mol M.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

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2015

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tom 10

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nr 2

Ograniczanie wyników

Phosphomannose isomerase gene for selection in lettuce [Lactuca sativa L.] transformation

Genetic translocation of resistance determinants in Staphylococcus aureus

Filter paper inhibits in vitro protocorm-like body formation in hybrid Cymbidium and reduces synseed germination, but buffers the negative impact of antibiotics

Differentiation of adventitious buds from Capsicum annuum L. hypocotyls after co-culture with Agrobacterium tumefaciens

Fertile transgenic Nicotiana benthamiana plants carrying capsid protein cDNA of plum pox virus

Effects of the secondary metabolite producing Pseudomonas fluorescens CHA0 on soil Protozoa and bacteria

Transformation of diploid potato with an Agrobacterium tumefaciens binary vector system: I. Methodological approach

Antybiotykoterapia u świń cz.4. Antybiotyki aminoglikozydowe