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Wounding stimulates allene oxide synthase gene and increases the level of jasmonic acid in Ipomoea nil cotyledons

100%

Wilmowicz E., Kucko A., Frankowski K., Zabrocka-Nowakowska B., Panek K., Kopcewicz J.

Acta Societatis Botanicorum Poloniae

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2016

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tom 85

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nr 1

Allene oxide synthase (AOS) encodes the first enzyme in the lipoxygenase pathway, which is responsible for jasmonic acid (JA) formation. In this study we report the molecular cloning and characterization of InAOS from Ipomoea nil. The full-length gene is composed of 1662 bp and encodes for 519 amino acids. The predicted InAOS contains PLN02648 motif, which is evolutionarily conserved and characteristic for functional enzymatic proteins. We have shown that wounding led to a strong stimulation of the examined gene activity in cotyledons and an increase in JA level, which suggest that this compound may be a modulator of stress responses in I. nil.

2

Impact of jasmonates, salicylic acid, and fungal elicitor on the health of Hippeastrum hybridum bulbs achieved by in vitro procedure

100%

Trejgell A., Szmidt-Jaworska A.

Acta Agrobotanica

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2024

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tom 77

3

Auxin increases the InJMT expression and the level of JAMe–inhibitor of flower induction in Ipomoea nil

84%

Kucko A., Czeszewska-Rosiak G., Wolska M., Glazinska P., Kopcewicz J., Wilmowicz E.

Acta Societatis Botanicorum Poloniae

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2017

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tom 86

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nr 1

Interactions among jasmonates and auxin in the photoperiodic flower induction of a short-day plant Ipomoea nil were examined. Therefore, we measured changes in jasmonic acid (JA) and jasmonic acid methyl ester (JAMe) levels in the cotyledons of I. nil during the inductive night, as well as the effects of indole-3-acetic acid (IAA) on their content. We noticed an interesting result, that IAA applied on the cotyledons of I. nil is an effective stimulator of JAMe production in seedlings cultivated under inductive night conditions. IAA treatment also significantly increased the transcriptional activity of InJMT (JASMONIC ACID CARBOXYL METHYLTRANSFERASE), while did not affect the expression of JA biosynthesis genes (lipoxyganease, allene oxide synthase, 12-oxophytodienoate reductase). These data, as well as the results of our previous research, suggest that exogenous IAA participates in I. nil flower induction process by stimulating InJMT expression and, as a consequence of that, enhancing the level of JAMe, a flowering inhibitor.

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The role of jasmonates in the formation of a compound of chalcones and flavans with phytoalexin-like properties in mechanically wounded scales of Hippeastrum × hybr. bulbs

84%

Wilmowicz E., Frankowski K., Grzegorzewska W., Kesy J., Kucko A., Banach M., Szmidt-Jaworska A., Saniewski M.

Acta Biologica Cracoviensia. Series Botanica

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2014

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tom 56

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nr 1

5

Changes in jasmonates and 12-oxophytodienoic acid contents of Medicago sativa L. during somatic embryogenesis

84%

Rudus I., Kepczynska E., Kepczynski J., Wasternack C., Miersch O.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 4A

Jasmonic acid (JA), its methyl ester (MeJA) and the biosynthetic precursor 12-oxophytodienoic acid (OPDA) were detected quantitatively during somatic embryogenesis of Medicago sativa L. Using GC-MS analysis, these compounds were found in initial explants, in calli and in somatic embryos in the nanogram range per gram of fresh weight. In distinct stages of somatic embryogenesis, JA and 12-OPDA accumulated preferentially in cotyledonary embryos. Initial explants exhibited about five-fold higher JA content than OPDA content, whereas in other stages OPDA accumutated predominantly. These data suggest that also in embryogenic tissues OPDA and JA may have individual signalling properties.

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Relationships between jasmonates and auxin in regulation of some physiological processes in higher plants

84%

Saniewski M., Ueda J., Miyamoto K.

Acta Physiologiae Plantarum

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2002

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tom 24

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nr 2

7

Relationship between jasmonates and ethylene in regulation of some physiological processes in plants under stress conditions

67%

Saniewski M., Ueda J., Miyamoto K., Urbanek H.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2002

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tom 481

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nr 1

A relationship between jasmonates and ethylene in regulation of some physiological processes in plants under stress conditions is presented. Jasmonates are naturally occurring plant hormones showing various important biological activities in the regulation of plant growth development and in defense responses against a wide variety of abiotic and biotic agents. Jasmonates have been reported to control ethylene biosynthesis in intact plants and their organs. Mechanical wounding and other abiotic (osmotic stress, water deficit, dessication stress, heavy metals, touch, ozone) and biotic stresses (pathogen infection and insect invasion) are well known to be common factors inducing ethylene and jasmonates biosynthesis, and reactive oxygen species generation (ROS). Jasmonates have been well known to interact with ethylene in regulation of different processes; various kinds of interactions were documented: 1) synergistic interaction (i.e gene expression of proteinase inhibitors, osmotin, defensin), 2) ethylene suppresses processes induced by jasmonates (i.e. biosynthesis of nicotine, vegetative storage proteins and lectins), 3) jasmonates suppress processes induced by ethylene (i.e. ethylene-induced apical hook). Jasmonic acid carboxyl methyltransfe- rase (JMT) is a key enzyme for jasmonate-regulated plant responses. Activation of JMT expression leads to production of methyl jasmonate (JA-Me). JA-Me can act as an intracellular regulator, a diffusible intercellular signal transducer, or an airborne signal mediating intra- and interplant communications. Jasmonates represent an integral part of the signal transduction chain between stress signals) and stress responses(s), in most cases of the induction of gene expression and the accumulation of defense specific proteins and secondary metabolites.

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Effects of Cu2plus, cytokinins and jasmonate on content of two flavonols identified in zucchini cotyledons

67%

Stoynova-Bakalova E., Nikolova M., Maksymiec W.

Acta Biologica Cracoviensia. Series Botanica

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2009

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tom 51

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nr 2

77-83

This study examined the effects of cytokinins and methyl jasmonate (JAMe) alone and in combination on the growth and flavonol content of zucchini cotyledons cultured in the presence or absence of Cu2+. During greening of cotyledons in intensive diurnal illumination, rutin was identified as the main flavonol compound. Its accumulation was greatly stimulated by phenylurea cytokinin (4PU-30) but reduced by Cu2+. Stimulation by benzylaminopurine (BA) was less. JAMe showed an inhibitory effect, alone and with simultaneous addition of Cu2+ or cytokinins. In moderate excess (100 μM and 250 μM CuSO4), Cu2+ enhanced stimulation by 4PU-30 of rutin accumulation; at a higher concentration or with other substances it decreased the rutin level. The other flavonol detected, kaempferol-3-rhamnoside, increased after JAMe or 4PU-30 were added; in the other cases it decreased. The data obtained indicate that cytokinins can increase rutin content in developing Cucurbita cotyledons. Rutin content usually decreased under stress induced by excess Cu2+, but Cu2+ in moderate excess had a stimulating effect in the presence of higher phenylurea cytokinin levels

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Hormonal control of gummosis in Rosaceae

67%

Saniewski M., Ueda J., Miyamoto K., Horbowicz M., Puchalski J.

Journal of Fruit and Ornamental Plant Research

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2006

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tom 14

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nr Suppl.1

10

Wpływ elicytacji jasmonianem metylu na akumulację kwasów fenolowych w kulturach zawiesinowych Ginkgo biloba L.

59%

Szewczyk A.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2008

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tom 524

Określono wpływ jasmonionianu metylu na akumulację fenolokwasów w kulturach zawiesinowych Ginkgo biloba. Kultury okazów żeńskich prowadzono na podłożu Murashige - Skoog’a z dodatkiem pikloramu (4 mg·dm⁻³) oraz BA (2 mg·dm⁻³). Jasmonian metylu w stężeniu 25 µM dodawano w 14 dniu od założenia hodowli. Analizy ekstraktów metanolowych z biomasy prowadzono metodą HPLC. Detekcję aglikonów fenolokwasów prowadzono po hydrolizie ich glikozydów 2 mg·dm⁻³ wodnym roztworem HCl. Wyniki wskazują, że dodatek jasmonianu metylu może stymulować kultury zawiesinowe Ginkgo biloba do zwiększenia produkcji kwasów fenolowych, szczególnie ferulowego i syryngowego.

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Wpływ inhibitorów biosyntezy jasmonianów na zawartość chlorofilu i antocyjanów w pędach Clematis rosnących in vitro

51%

Wegrzynowicz-Lesiak E., Kawa-Miszczak L., Gabryszewska E., Goraj J., Saniewski M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2010

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tom 545

Badano wpływ jasmonianu metylu (JA-Me) i inhibitorów biosyntezy jasmonianów, ibuprofenu i galusanu propylu, na akumulację chlorofilu i antocyjanów w pędach Clematis pitcheri rosnących w warunkach in vitro na pożywce MS. Doświadczenia prowadzono w 20°C i 25°C. Do pożywki dodawano JA-Me w stężeniu 1 µМ, ibuprofen 1 µM i 10 µМ, galusan propylu 100 µM lub ich mieszaniny. Pędy rosnące w temperaturze 20°C zawierały więcej chlorofilu i akumulowały więcej antocyjanów niż w 25°C. Dodanie do pożywki JA-Me nie wpływało na zawartość chlorofilu w pędach zarówno w 20°C jak i 25°C. JA-Me nie miał wpływu na akumulację antocyjanów w pędach rosnących w 20°C i w niewielkim stopniu obniżał poziom antocyjanów w pędach rosnących w 25°C w porównaniu do kontroli. Zastosowanie inhibitorów biosyntezy jasmonianów znacznie zwiększało zawartość chlorofilu, w porównaniu do roślin kontrolnych i rosnących na pożywce z dodatkiem JA-Me. Równoczesne podanie JA-Me i galusanu propylu zwiększało zawartość chlorofilu w pędach rosnących w 20°C w porównaniu do traktowania samym galusanem propylu. Kiedy JA-Me był podany łącznie z ibuprofenem, pędy rosnące w 25°C zawierały mniej chlorofilu niż na pożywce z samym ibuprofenem. Ibuprofen dodany do pożywki w stężeniu 10 µM stymulował akumulację antocyjanów w pędach w obu temperaturach. Kiedy JA-Me był podany łącznie z ibuprofenem lub galusanem propylu pędy akumulowały mniej antocyjanów niż na pożywkach z inhibitorami podanymi osobno.

Ograniczanie wyników

Wounding stimulates allene oxide synthase gene and increases the level of jasmonic acid in Ipomoea nil cotyledons

Impact of jasmonates, salicylic acid, and fungal elicitor on the health of Hippeastrum hybridum bulbs achieved by in vitro procedure

Auxin increases the InJMT expression and the level of JAMe–inhibitor of flower induction in Ipomoea nil

The role of jasmonates in the formation of a compound of chalcones and flavans with phytoalexin-like properties in mechanically wounded scales of Hippeastrum × hybr. bulbs

Changes in jasmonates and 12-oxophytodienoic acid contents of Medicago sativa L. during somatic embryogenesis

Relationships between jasmonates and auxin in regulation of some physiological processes in higher plants

Relationship between jasmonates and ethylene in regulation of some physiological processes in plants under stress conditions

Effects of Cu2plus, cytokinins and jasmonate on content of two flavonols identified in zucchini cotyledons

Hormonal control of gummosis in Rosaceae

Wpływ elicytacji jasmonianem metylu na akumulację kwasów fenolowych w kulturach zawiesinowych Ginkgo biloba L.

Wpływ inhibitorów biosyntezy jasmonianów na zawartość chlorofilu i antocyjanów w pędach Clematis rosnących in vitro