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Based on distinct colony morphology, color, size, shape and certain other traits, 92 bacterial isolates were investigated to understand their managerial ability on iron from the Arabian Sea and Equatorial Indian Ocean samples. The ARDRA (amplified rDNA restriction analysis) applied to eliminate the duplication of the bacterial strains, resulted 39 different banding patterns. The 16S rRNA gene sequencing data indicate the dominancy of three phylogenetic groups, α-Proteobacteria (10.25%), γ-Proteobacteria (35.89%) and Bacilli (53.84%) in these waters. Marinobacter and Bacillus were the only common genera from both of the regions. Pseudoalteromonas, Halomonas, Rheinheitnera, Staphylococcus and Idiomarina were some of the other genera obtained from the Arabian Sea. Erythrobacter, Roseovarius, Sagittula and Nitratireductor were found mostly in Equatorial Indian Ocean. In addition, 16S rRNA gene sequence data of some of our iron bacterial strains belong to novel species and one isolate ASS2A could form a new genus. Close to 23% of the isolates were able to produce high affinity sets of ligands like siderophores to mediate iron transport into the cell. The current study indicated that the Equatorial Indian Ocean species were well adapted to oxidize iron as an electron acceptor and the Arabian Sea species preferably go through siderophore production.
The results of three-year investigations with cv. Galaxy F1 cabbage grown commercially in an important agricultural region of South Poland are presented. The effect of the rate of ammonium sulphate and UAN (solution of ammonium nitrate + urea), the method of application (placement and broadcast technique) and foliar fertilization (urea and Supervit K) on Cu, Mn, Zn, Fe, B and Mo concentrations in edible parts of cabbage were surveyed. Nitrogen fertilizer was applied at the rate of 120 kg N ha–1. With the placement fertilization method, fertilizer was applied in rows 10 cm deep and 10 cm away from each plant when seedlings were transplanted. Foliar sprayings started at the beginning of intensive leaf growth and continued during the growing season at two-week interval. The foliar nutrition with 2% urea was carried out 3 times and 1% Supervit K was applied once. The field experiment was carried out in 2005-2007 with cv. Galaxy F1 white cabbage on silty clay soil containing 0.91-1.02% organic carbon and soil acidity pHH2O 7.18-8.21 Micronutrient concentrations were below the lower range of the content reported for cabbage grown in non-contaminated areas. Ammonium sulphate significantly increased Mn and Fe concentrations in cabbage heads and decreased B and Mo content. However, the environmental factors considerably modified this tendency. The method of N application affected Mn content in cabbage in 2007 and Mo in 2006 and 2007. Slightly higher manganese and molybdenum concentrations for placement fertilization were noted. In 2005 and 2006, the placement fertilization at the rate of 75% N and supplemented foliar sprays increased Mo content in cabbage.
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