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1

Beta3-integrin cytoplasmic binding proteins

100%
Zhao R., Pathak A. S., Stouffer G. A.
Archivum Immunologiae et Therapiae Experimentalis
|
2004
|
tom 52
|
nr 5
2

Effect of alpha3beta1 and alphavbeta3 integrin glycosylation on interaction of melanoma cells with vitronectin

86%
Janik M. E., Przybylo M., Pochec E., Pokrywka M., Litynska A.
Acta Biochimica Polonica
|
2010
|
tom 57
|
nr 1
55-61
 The metastatic transformation of melanocytes is associated with altered expression of adhesion molecules, including αvβ3 and α3β1 integrins. Integrin αvβ3 is a primary vitronectin (VN) receptor, while both integrin types take part in adhesion to VN when they are in complex with uPAR. Although their role in melanoma cell interaction with VN is of great interest, the influence of N-oligosaccharides attached to these glycoproteins is still unappreciated. The present study assesses the role of αvβ3 and α3β1 integrins and the influence of their glycosylation status on WM9 and WM239 metastatic melanoma cell interactions with VN. Cell adhesion to and migration on VN were selected as the studied cell behaviour parameters. Functionblocking antibodies and swainsonine (SW) treatment were used in these tests. Both cell lines interacted with VN in an integrin-mediated but cell-line-specific manner. In WM9 cells, migration was not completely inhibited by antibodies against α3β1 or αvβ3 integrins, suggesting the participation of other VN receptors. In both cell lines in coprecipitation test the formation of an integrins/uPAR complex was shown. In the presence of SW formation of the complex did not occur, suggesting the participation of glycosylation in this proccess. Additionally, the adhesion properties of WM9 cells were changed after SW treatment. Our results suggest that in these two metastatic cell lines integrin-linked N-oligosaccharides influence the VN adhesion receptor activity and function.
3

Trafficking of FoxP3plus regulatory T cells: myths and facts

86%
Kim C. H.
Archivum Immunologiae et Therapiae Experimentalis
|
2007
|
tom 55
|
nr 3
4

Regulation of beta1 integrin expression in endothelial cells by chimeric tRNA Va1 ribozyme

86%
Papiewska-Pajak I., Antoszczyk S.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr 2
To downregulate expression of the β1 integrin subunit in endothelial cells, plasmid encoding the ribozyme cassette containing hammerhead ribozyme flanked at the 5' terminus by tRNAVal and at the 3' terminus by constitutive transport element sequences was constructed. When used to transfect immortalized human endothelial cell line EA.hy 926, it selectively blocked the synthesis of the β1 integrin subunit and thus inhibited migration and proliferation of the cells. Thus, this construct may be a valuable tool to control the proangiogenic phenotype of stimulated endothelial cells.
5
Content available remote

Collagen receptors alpha1beta1 and alpha2beta1 integrins are involved in transmigration of peripheral blood eosinophils, but not mononuclear cells through human microvascular endothelial cells monolayer

72%
Bazan-Socha S., Zuk J., Plutecka H., Marcinkiewicz C., Zareba L., Musial J.
Journal of Physiology and Pharmacology
|
2012
|
tom 63
|
nr 4
6
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Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period

72%
Bukowska D., Kempisty B., Jackowska M., Wozna M., Antosik P., Piotrowska H., Jaskowski J. M.
Polish Journal of Veterinary Sciences
|
2011
|
tom 14
|
nr 2
Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.
7

GSL-enriched membrane microdomains in innate immune responses

72%
Nakayama H., Ogawa H., Takamori K., Iwabuchi K.
Archivum Immunologiae et Therapiae Experimentalis
|
2013
|
tom 61
|
nr 3
8

T cell integrin activation by chemokines in inflammation

72%
Tanaka Y.
Archivum Immunologiae et Therapiae Experimentalis
|
2000
|
tom 48
|
nr 6 Spec.Iss.
443-450
9

Expression of beta1-integrins and N-cadherin in bladder cancer and melanoma cell lines

72%
Laidler P., Gil D., Pituch-Noworolska A., Ciolczyk D., Ksiazek D., Przybylo M., Litynska A.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 4
Changes in the expression of integrins and cadherins might contribute to the progression, invasion and metastasis of transitional cell cancer of the bladder and of melanomas. The expression of alpha5 (P<0.001), alpha2 and beta1 (P<0.05-P<0.001) integrin subunits in melanoma cells from noncutaneous metastatic sites (WM9, A375) were significantly increased as compared to cutaneous primary tumor (WM35) and metastatic (WM239) cell lines. These differences might be ascribed to the invasive character of melanoma cells and their metastasis to the noncutaneous locations. The significantly heterogeneous expression of beta1 integrin subunit in two malignant bladder cancer cell lines (T24 and Hu456) and nonsignificant differences in the expression of alpha2, alpha3, and alpha5 subunits between malignant and non-malignant human bladder cell lines do not allow an unanimous conclusion on the role of these intergrin subunits in the progression of transitional cancer of bladder. The adhesion molecule, expressed in all studied melanoma and bladder cell lines, that reacted with anti-Pan cadherin monoclonal antibodies was identified as N-cadherin except in the HCV29 non-malignant ureter cell line. However, neither this nor any other bladder or melanoma cell line expressed E-cadherin. The obtained results imply that the replacement of E-cadherin by N-cadherin accompanied by a simultaneous increase in expression of alpha2, alpha3 and alpha5 integrin subunits clearly indicates an increase of invasiveness of melanoma and, to a lesser extent, of transitional cell cancer of bladder. High expression of N-cadherin and alpha5 integrin subunit seems to be associated with the most invasive melanoma phenotype.
10

Tumor cell N-glycans in metastasis

72%
Laidler P., Litynska A.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 2
Metastasis accounts for most of deaths caused by cancer. The increasing body of evidence suggests that changes in N-glycosylation of tumor cell proteins such as increased branching, increased sialylation, polysialylation, decreased fucosylation, enhanced formation of Lewis X and sialyl Lewis X antigens are among important factors determining metastatic potential of tumor cell. Most of the adhesion proteins, e.g., integrins, members of immunoglobulin superfamily, and cadherins are heavily N-glycosylated. The other proteins involved in adhesion, like galectins and type-C selectins, recognize N-glycans as a part of their specific ligands. In this review we focus on recent reports concerning the contribution of N-glycosylation of tumor cell adhesion molecules and some selected membrane proteins in the tumor invasion and metastasis.
11

Signal transduction in confluent C3H 10T1-2 cells. The role of focal adhesion kinase

72%
Miloszewska J., Trembacz H., Janik P.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 1
The activities of extracellular signal-regulated kinases (ERK1/ERK2) is required for proliferation of several types of cells. The performed analysis showed stimulation of ERK's by fetal calf serum (FCS) or fibronectin in the C3H 10T1/2 cell cultures at logarith­mic phase of growth. The ERKs activity was not stimulated in confluent cells. This could not be accounted for a partial down regulation of ERK since its level was stable in both types of cells regardless of their density and kind of stimulation. Searching for ERK up­stream elements we studied the integrin receptor gene transcript by RT-PCR and focal ad­hesion kinase (FAK) by Western blotting and phosphorylation assays. It was found that FCS and fibronectin stimulated phosphorylating activity of FAK in the cells at the logarithmic phase of growth, but were inefficient in the confluent cells. RT-PCR showed the presence of α5 and β1 integrin transcripts, and p125FAK was at the same level regardless of the type of stimulation. These data indicate that the ability of FAK to be activated plays an important role in ERK regulation and, in consequence in proliferation and growth inhibition during confluence.
12

DNAzyme as an efficient tool to modulate invasiveness of human carcinoma cells

58%
Wiktorska M., Papiewska-Pajak I., Okruszek A., Sacewicz-Hofman I., Niewiarowska J.
Acta Biochimica Polonica
|
2010
|
tom 57
|
nr 3
 In this study we evaluated efficiency of DNAzymes to modulate motility of cancer cells, an important factor in the progression and metastasis of cancers. For this purpose we targeted β1 integrins that are predominant adhesive receptors in various carcinoma cell lines (CX1.1, HT29, LOVO, LS180, PC-3). To evaluate invasiveness of cancer cells, we used a transwell migration assay that allowed analyzing chemotactic migration of colon carcinoma cell lines across an ECM-coated membrane. Their adhesive properties were also characterized by the analysis of adhesion to fibronectin, laminin and collagen. In addition, the expression of major integrin subunits, selected intact β1 integrins, and other adhesive receptors (ICAM, E-selectin, uPAR) was analyzed by flow cytometry. Inhibition of β1 integrin expression by DNAzyme to β1 mRNA almost abolished the invasiveness of the CX1.1, HT29, LS180, LOVO and PC-3 cells in vitro. These data show that DNAzymes to β1 integrin subunit can be used to inhibit invasiveness of carcinoma cells.
13

CA-125 of fetal origin can act as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin

58%
Mitic N., Milutinovic B., Jankovic M.
Cellular and Molecular Biology Letters
|
2014
|
tom 19
|
nr 2
CA-125 (coelomic epithelium-related antigen) forms the extracellular portion of transmembrane mucin 16 (MUC16). It is shed after proteolytic degradation. Due to structural heterogeneity, CA-125 ligand capacity and biological roles are not yet understood. In this study, we assessed CA-125 as a ligand for dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), which is a C-type lectin showing specificity for mannosylated and fucosylated structures. It plays a role as a pattern recognition molecule for viral and bacterial glycans or as an adhesion receptor. We probed a human DC-SIGN-Fc chimera with CA-125 of fetal or cancer origin using solid- or fluid-phase binding and inhibition assays. The results showed that DC-SIGN binds to CA-125 of fetal origin and that this interaction is carbohydrate-dependent. By contrast, cancerderived CA-125 displayed negligible binding. Inhibition assays indicated differences in the potency of CA-125 to interfere with DC-SIGN binding to pathogen-related glycoconjugates, such as mannan and Helicobacter pylori antigens. The differences in ligand properties between CA-125 of fetal and cancer origin may be due to specificities of glycosylation. This might influence various functions of dendritic cells based on their subset diversity and maturation-related functional capacity.
14

Muscle-specific integrins in masseter muscle fibers of chimpanzees: an immunohistochemical study

58%
Favaloro A., Speranza G., Rezza S., Gatta V., Vaccarino G., Stuppia L., Festa F., Anastasi G.
Folia Histochemica et Cytobiologica
|
2009
|
tom 47
|
nr 4
15

Adhesion properties of human bladder cell lines with extracellular matrix components: the role of integrins and glycosylation

58%
Litynska A., Przybylo M., Pochec E., Laidler P.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 3
Integrin subunits present on human bladder cells displayed heterogeneous func­tional specificity in adhesion to extracellular matrix proteins (ECM). The non-malignant cell line (HCV29) showed significantly higher adhesion efficiency to collagen IV, laminin (LN) and fibronectin (FN) than cancer (T24, Hu456) and v-raf transfected (BC3726) cell lines. Specific antibodies to the a2, a5 and β1 integrin sub- units inhibited adhesion of the non-malignant cells, indicating these integrin partici­pation in the adhesion to ECM proteins. In contrast, adhesion of cancer cells was not inhibited by specific antibodies to the β1 integrin subunit. Antibodies to a3 integrin in­creased adhesion of cancer cells to collagen, LN and FN, but also of the HCV29 line with colagen. It seems that a3 subunit plays a major role in modulation of other integrin receptors especially in cancer cells. Differences in adhesion to ECM proteins between the non-malignant and cancer cell lines in response to Gal and Fuc were not evident, except for the v-raf transfected cell line which showed a distinct about 6-fold increased adhesion to LN on addition of both saccharides. .-Acetylneuraminic acid inhibited adhesion of all cell lines to LN and FN irrespective of their malignancy.
16
Content available remote

Elucidating structure-function relationships from molecule-to-cell-to-tissue: from research modalities to clinical realities

51%
Dobrucki L. W., Marsh B. J., Kalinowski L.
Journal of Physiology and Pharmacology. Supplement
|
2009
|
tom 60
|
nr 4
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