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The fraction of sterol acetates from the inflorescences of Hieracium pilosella has been isolated in the typical way from petroleum ether extract. By means of the weight method the total amount of sterols was determined (0.2659%). The mixtures of sterol acetates and free sterols were investigated using GC-MS techniques. The occurrence of about 18 sterols has been observed. Cholesterol, cholest-8(14)-en-3b-ol, cholesta-5.7-dien-3b-ol, cholest-7-en-3b-ol, ergosta-5.24-dien-3b-ol, campesterol, stigmasterol, b-sitosterol, fucosterol, 5a-stigmast-7-en-3a-ol were identified. The probable structures of lophenol, isofucosterol, 5a-stigmasta-7.24-dien-3b-ol, lanosta-9(11).24-dien-3b-ol and 24-ethylidene lophenol were stated on the basis of literature data. The last 4 sterols occur in a vestigial quantity, which made its identification impossible. Sitos erol and cholesterol are remarkably dominating sterols in the fraction.
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Origin of synthetic particles in honeys

100%
A total of 47 honeys and 22 flowering plants was analysed for their load of synthetic fibres and fragments. In all samples investigated foreign particles were found. These include also black carbon particles which were not enumerated. Fibres and fragments ranged from 10 to 336 kg-1 and 2 to 82 kg-1 honey, respectively. The data of the flowering plants analysed indicate that a major proportion of the particle load may originate from external sources, i.e. these particles are brought into the beehive by the worker bees during nectar collection.
Studies on flowering of Hierochloë repens were performed over the years 2005-2007 in five habitats located in Bydgoszcz. The first habitat is a degenerated fresh ryegrass meadow (Arrhenatheretum elatioris). Another one is a degenerated form of initial xerothermic swards (Tunico-Poetum compressae). The third habitat in which the occurrence of Hierochloë repens was found and flowering was analyzed is sandy sward (Koelerio-Corynephoretea). Yet another habitat is ruderal in character and it was located on the side of the road (Convolvulo-Brometum inermis). The last one is a fertile and moist habitat of elm-ash marshy meadow being gramineous herb fringe communities in character. The evaluation was based on the following analyses: morphological characters of inflorescences (length of inflorescence, stem length, number of inflorescence branches and number of spikelets), stem and inflorescence weight and reproduction effort as well as the share of generative individuals in the population and the number of generative individuals per m². Differences were found in the structure of Hierochloë repens inflorescence in the habitats analyzed. The biggest inflorescence, in terms of all the characters analyzed, was found in fertile elm-ash marshy meadow, and the smallest in poor xerothermic sward. The highest generative reproduction potential of Hierochloë repens was found in the ruderal habitats. They are the basic potential source of generative diaspora production, which can affect the spreading of this species in Poland.
The research on the flowering of Arnica montana L. and A. chamissonis Less, was carried out in the years 2005-2006 on two- and three-year plantations on grey-brown podsolic soil with the granulometric composition of heavy clay sand. The aim of the work was to determine the pattern and length of flowering of two- and three-year-old plants under field cultivation conditions with successive flower head collection. The flowering of Arnica montana in both years of study lasted 26 days (from the 28th of May to the 22nd of June). In this period, eight collections of flower heads were made. When analyzing the number of inflorescences gathered during particular collections, it was concluded that at the initial stage of flowering it was not great, but it successively increased with the passage of time up to the seventh collection. The pattern of flowering of the two- and three-year-old Arnica montana plants was similar. The inflorescences of Arnica chamissonis were collected six times, and the most abundant flowering was found in the middle period of this stage, which lasted 20 days.
Studies on sexual dimorphism in reference to vegetative features and on the sex ratio in different environmental conditions were carried out in lowland populations of dioecious species Aruncus sylvestris. It was shown that female individuals of Aruncus sylvestris produced higher flowering shoots, longer inflorescences, more leaves and longer and broader leaves than the male ones. All listed differences were statistically significant. In populations of Aruncus sylvestris growing in optimum ecological conditions (fertile and wet soil, half-light) the sex ratio M/F is close to 1:1. Changes of ecological conditions along the gradient of fertility, habitat moisture (estimated by the types of phytocoenoses) and intensity of solar radiation caused marked drift of sex structure towards the male domination. Light intensity was the crucial factor for the sex ratio in studied populations. Populations growing in half-light showed sex ratio M/F ≈ 1:1 irrespective of habitat. Those growing in shade and in full light showed significant shift to male dominance. The largest deviation from 1:1 sex ratio (M/F ≈ 1.0:0.2) was observed in extremely unfavorable habitat conditions (full sun irradiance, dry soil, strong competition from Elymus repens (L.) Gould). Obtained results demonstrated that female individuals of Aruncus sylvestris had markedly higher habitat requirements compared with the male ones.
In this study, we present investigations of morphological changes in the inflorescence of bolting Allium sativum L., which forms umbel-like inflorescences with small, inconspicuous flowers, bulbils, and leaf-like membranous bracts. Particular attention was focused on the architecture of a single flower, and the full sequence of developmental events and the differentiation of flower elements and inflorescence were traced. During the several-year long investigations of the garlic species, we observed that all inflorescence elements emerged chaotically, taking a completely random place in the inflorescence receptacle and it was impossible to identify the sequence of formation of the individual inflorescence elements. The absence of regular phyllotaxis and the specific sequence of formation of inflorescence elements are characteristic for inflorescence reversion. Since domesticated garlic has lost the capacity for sexual reproduction, it has probably evolved alternative reproduction modes in its developmental strategy, which is reflected by the formation of numerous bulbils (vegetative buds, topsets) in the inflorescence. This is one of the forms of reproductive strategy of the species, which through domestication progresses from sexual to asexual reproduction.
Molinietum caeruleae meadows belong to the most endangered plant communities in Central Europe, nowadays suffering due to the lack of management. The land abandonment promotes strong secondary expansion of macroforbs, sedges or willows, contributing to decrease of the species diversity. In light of these findings very interesting seems to be the evaluation of the variability of features of populations of rare meadow species. In Poland one of strictly protected plant is Gladiolus imbricatus L. It is a tuberous, clonal plant with sword-like leaves and tall stalks bearing purple flowers collected in one-sided inflorescence. The studies of spatio-temporal variability of selected features of subpopoulations of Gladiolus imbricatus were carried out in the years 2009-2011 in Kraków-Kostrze (Southern Poland) in patches of Molinietum caeruleae with different species composition. In Patch I chiefly occurred small meadow species, Patch II was prevailed by shrub willow, Patch III was dominated by tall-growing macroforbs, Patch IV was characterized by substantial share of large-tussock grasses, while Patch V was overgrown by tall rhizomatous grass Phragmites communis. The plant canopy height and ground moisture raised from Patch I, via Patches II, III and IV, to Patch V. Moreover, in all sites the above mentioned parameters increased moderately in consecutive seasons. The decline of abundance of subpopulations observed in the consecutive years and in the successive patches might be caused by a mortality of individuals, a physical damage of tubers, as well as a prolonged dormancy of genets. The structure of developmental stages were similar in consecutive years but differed significantly among Patches. The increasing trend toward senility noted in successive sites could be an effect of lack of microsites suitable for seedling recruitment or absence of vegetative regeneration. The dimensions of leaf blades, flowering stalks and inflorescences remained similar in the subsequent seasons, but they augmented significantly in the successive patches. The length and the width of capsules did not change significantly between seasons and sites. Concluding, it might be assumed, that the perspectives for survivability of subpopulations diminish gradually from Patch I, via Patches II, III and IV to Patch V. Simultaneously, it should be pointed out that prospects for persistence in each place steadily decrease in effect of continuous deterioration of site conditions.
The analysis of the fractions of free and bound, liberated by hydrolysis, phenolic acids in the inflorescences and rhizomes with roots of Hieracium pilosella L. was conducted by 2D-TLC and RP-HPLC methods. In the inflorescences fourteen phenolic acids were found: chlorogenic, gallic, protocatechuic, homoprotocatechuic, caffeic, gentisic, p-hydroxyphenylacetic, p-hydroxybenzoic, m-hydroxybenzoic, p-coumaric, syringic, vanillic, ferulic and salicylic. In the rhizomes with roots nine phenolic acids were identified: chlorogenic, gallic, homoprotocatechuic, caffeic, gentisic, p- hydroxyphenylacetic, p-hydroxybenzoic, syringic and vanillic. By means of the RP-HPLC method the contents of major acids were estimated which ranged from 0.01 mg% up to 139.50 mg%. Caffeic acid is the remarkably dominating constituent of both plant materials. Phenolic compounds may contribute to invasiveness and medicinal properties of this species.
Celem naszej pracy było określenie zawartości procentowej i składu chemicznego olejków eterycznych występujących w kwiatostanach, liściach i kłączach Silphium perfoliatum L. Zawartość procentową olejku oznaczano metodami destylacji z parą wodną w aparacie Derynga wg FP IV i FP V. Skład olejków eterycznych otrzymanych z kwiatostanów i liści jest podobny z tym, że w przypadku kwiatostanów dominującym związkiem w olejku jest a-pinen (20,9%), zaś w przypadku liści głównym składnikiem jest tlenek kariofilenu (34,7%). Natomiast skład olejków eterycznych otrzymanych z kłączy różni się istotnie od składu olejków otrzymanych z liści i kwiatostanów.
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