The aim of this work was to investigate the applicability of proteolytic enzymes complex (consisted of chymotrypsin, carboxypeptidase A and leucine aminopeptidase) immobilised in polyvinyl alcohol gel for hydrolysis of casein. Inversely proportional relationship between the extent of aromatic amino acids release and the concentration of casein was found. After 48 h of hydrolysis, the catalytic activities of enzymes in immobilised and native forms were equal, what is especially attractive in the case of immobilised form, bearing in mind well known advantages of biocatalysts in that form. The hydrolysis performed in column filled with immobilised enzymes resulted in as high degree of casein degradation as 80%. The high stability of obtained biocatalyst was confirmed by its 54 days half-life period.