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  1. 16 Archivum Immunologiae et Therapiae Experimentalis

  2. 3 Acta Parasitologica

  3. 3 Journal of Physiology and Pharmacology. Supplement

  4. 3 Polish Journal of Veterinary Sciences

  5. 1 Acta Microbiologica Polonica

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  1. 10 Wieczorek Z.

  2. 8 Siemion I. Z.

  3. 8 Zimecki M.

  4. 3 Demkow U.

  5. 3 Filewska M.

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  1. 1 2021

  2. 2 2017

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  4. 1 2014

  5. 1 2012

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1

Functional maturation of thymocytes: recruitment of helper cells in the humoral immune response to SRBC in vitro by recombinant interleukin 1 (Il-1)

100%
Zimecki M., Wieczorek Z.
Archivum Immunologiae et Therapiae Experimentalis
|
1989
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tom 37
|
nr 3-4
2

Characterisation of a new molecule based on two E2 sequences from bovine viral diarrhoea-mucosal disease virus fused to the human immunoglobulin Fc fragment

100%
Gonzalez Pose A., Montesino Segui R., Maura Perez R., Hugues Salazar F., Cabezas Avila I., Altamirano Gomez C., Sanchez Ramos O., Toledo J. R.
Journal of Veterinary Research
|
2021
|
tom 65
|
nr 1
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Humoral immune response in children with acute lymphoblastic leukaemia following influenza vaccination

100%
Brydak L. B., Rokicka-Milewska R., Jackowska T., Machala M.
Bulletin of the Veterinary Institute in Puławy
|
1998
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tom 42
|
nr 1
4

Immunomodulatory activity of conformationally restricted peptides related to TGF-beta2 90-99 sequence

100%
Wieczorek Z., Slon J. I., Siemion I. Z.
Archivum Immunologiae et Therapiae Experimentalis
|
1996
|
tom 44
|
nr 4
5

The effect of carbohydrate moiety structure on the immunoregulatory activity of lactoferrin in vitro

84%
Zimecki M., Artym J., Kocieba M., Duk M., Kruzel M. L.
Cellular and Molecular Biology Letters
|
2014
|
tom 19
|
nr 2
The aim of this study was to evaluate the immunoregulatory effects of recombinant human lactoferrin (rhLF) in two in vitro models: (1) the secondary humoral immune response to sheep erythrocytes (SRBC); and (2) the mixed lymphocyte reaction (MLR). We compared the non-sialylated glycoform of rhLF as expressed by glycoengineered Pichia pastoris with one that was further chemically sialylated. In an earlier study, we showed that sialylated rhLF could reverse methotrexate-induced suppression of the secondary immune response of mouse splenocytes to SRBC, and that the phenomenon is dependent on the interaction of lactoferrin (LF) with sialoadhesin (CD169). We found that the immunorestorative activity of sialylated rhLF is also dependent on its interaction with the CD22 antigen, a member of the immunoglobulin superfamily that is expressed by B lymphocytes. We also demonstrated that only sialylated rhLF was able to inhibit the MLR reaction. MLR was inhibited by bovine lactoferrin (bLF), a glycoform that has a more complex glycan structure. Desialylated bLF and lactoferricin, a bLF-derived peptide devoid of carbohydrates, did not express such inhibitory activity. We showed that the interaction of LF with sialic acid receptors is essential for at least some of the immunoregulatory activity of this glycoprotein.
6
Content available remote

Humoral immune response against mycobacterial antigens in children with tuberculosis

84%
Demkow U., Ziolkowski J., Bialas-Chromiec B., Filewska M., Zielonka T., Wasik M., Rowinska-Zakrzewska E.
Journal of Physiology and Pharmacology. Supplement
|
2006
|
tom 57
|
nr 4
63-73
Different clinical outcomes of tuberculosis (TB) are related to the balance between cell-mediated and humoral immunity and may depend on environmental and individual factors including age and sex. The purpose of this study was to analyze the humoral immune response to recombinant and native mycobacterial antigens in relation to clinical presentations of pulmonary TB in children. We examined 224 serum samples including 81 primary and 31 postprimary TB cases, 30 cases of latent TB infection, and 82 nontuberculosis controls. Commercially available ELISA assays detecting IgG, IgA, and IgM against antigens: 38 kDa, 16 kDa, 38kDa, lipoarabinomannan (LAM), and A-60 were used. The results indicate that IgG production was very low in primary compared with postprimary TB (P<0.0001). IgM levels did not differ between the examined groups. Antibody levels strongly depended on the child's age. In infants aged below 1 there was no difference in the antibody level between the TB and control cases. Most positive cases were observed in children aged above 10. The influence of BCG vaccination on the antibody level was not seen. In all subgroups, person-to-person heterogeneity of antigen recognition was observed. We conclude that humoral immune response is associated with the phase of TB and is stronger in more advanced TB forms. IgG and IgA production against mycobacterial antigens is very low in young children.
7
Content available remote

Heterogeneity of antibody response to mycobacterial antigens in different clinical manifestations of pulmonary tuberculosis

84%
Demkow U., Filewska M., Michalowska-Mitczuk D., Kus J., Jagodzinski J., Zielonka T., Zwolska Z., Wasik M., Rowinska-Zakrzewska E.
Journal of Physiology and Pharmacology. Supplement
|
2007
|
tom 58
|
nr 5
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The effect of florfenicol on lymphocyte subsets and humoral immune response in mice

84%
Lis M., Szczypka M., Suszko A., Switala M., Obminska-Mrukowicz B.
Polish Journal of Veterinary Sciences
|
2011
|
tom 14
|
nr 2
Florfenicol is a broad-spectrum bacteriostatic antibiotic used in domestic animals. The aim of the study was to determine the effect of florfenicol on the total number of lymphocytes in the thymus, spleen and mesenteric lymph nodes and the percentage and the absolute number of T cell subsets (CD4+CD8+, CD4-CD8-, CD4+, CD8+) in the thymus and T (CD3+, CD4+, CD8+) and B (CD19+) lymphocytes in the peripheral lymphatic organs in non-immunized mice and humoral immune response in sheep red blood cells (SRBC)-immunized mice. Florfenicol was administered orally at a dose of 30 mg/kg six times at 24 h intervals to non-immunized mice and four or seven times at 24 h intervals to SRBC-immunized mice. SRBC was injected 2 hours prior to the first dose of the drug. Florfenicol increased the percentage of CD4-CD8- thymocytes and the absolute number of CD4+ and CD8+ thymocytes on day 7. The increased percentage and absolute number of CD3+, CD4+ and CD8+ lymphocytes in mesenteric lymph nodes and decreased percentage of lymphocytes B were also observed 24 hours from the last administration of florfenicol. Florfenicol administered after SRBC immunization reduced the number of plaque forming cells (PFC) and the production of anti-SRBC antibodies on days 4 and 7 after priming.
9

Evaluation of specific humoral immune response in pigs vaccinated intradermally with deleted Aujeszky's disease vaccine and challenged with virulent strain of Herpesvirus suis type 1

84%
Mikulska-Skupien E., Szweda W., Procajlo Z.
Polish Journal of Veterinary Sciences
|
2005
|
tom 08
|
nr 1
10

Expression of FOXJ1 and ITGB4 is activated upon KLH and LTA stimulation in the DT40 cell line

84%
Dunislawska A., Slawinska A., Siwek M.
Folia Biologica
|
2017
|
tom 65
|
nr 1
11

A 43 kDa recombinant plasmepsin elicits immune response in mice against Plasmodium berghei malaria

84%
Pirta C., Sharma N. N., Banyal H. S.
Acta Parasitologica
|
2016
|
tom 61
|
nr 1
12
Content available remote

Humoral immune response against mycobacterial antigens in bronchoalveolar fluid from tuberculosis patients

84%
Demkow U., Bialas-Chromiec B., Filewska M., Sobiecka M., Kus J., Szturmowicz M., Zielonka T., Augustynowicz-Kopec E., Zwolska Z., Wasik M., Rowinska-Zakrzewska E.
Journal of Physiology and Pharmacology. Supplement
|
2005
|
tom 56
|
nr 4
79-84
Resistance to tuberculosis (TB) is cell-mediated but a humoral response is common and may be correlated with the lack of effective local cellular defense mechanisms. The goal of the study was to evaluate IgG, IgA, and IgM-mediated humoral immune response against 38-kDa+16-kDa and 38-kDa+lipoarabinomannan (LAM) mycobacterial antigens in bronchoalveolar fluid (BALF) from patients with pulmonary TB. Non-tuberculosis (NTB) patients were used as control. 179 BALF samples (56 TB and 123 NTB) were examined. Commercially available ELISA-based assays against proteins 38-kDa and 16-kDa or 38-kDa plus LAM were used. Three different dilutions of BALF: 1:1; 1:10, and 1:50 (100) were tested. Only the results obtained with the 1:10 dilution allowed distinguishing TB and NTB groups. The mean IgG level for 38-Da+LAM was significantly higher in the TB than that in the NTB group (P<0.0001). The mean IgA level for 38-kDa+LAM also was higher in the TB group (P<0.05). No difference was observed between TB and NTB groups in the titer of IgM antibodies. These findings indicate that TB is associated with the presence of detectable levels of antibodies in BALF. The antibody response is highly heterogeneous. This phenomenon results from the balance between pathogen and host immune system. The tests examined for detection of IgG in BALF can be used in combination with other diagnostic methods to increase diagnostic accuracy of pulmonary TB.
13

Differentiation of T cells into helper cells from immature precursors: identification of a target cell for a proline-rich polypeptide (PRP)

84%
Wieczorek Z., Zimecki M., Spiegel K., Lisowski J., Janusz M.
Archivum Immunologiae et Therapiae Experimentalis
|
1989
|
tom 37
|
nr 3-4
14

Humoral response of mice infected with Toxocara canis following different infection schemes

84%
Novak J., Panska L., Machacek T., Kolarova L., Horak P.
Acta Parasitologica
|
2017
|
tom 62
|
nr 4
15

Synthesis and immunosuppressive activity of glycine containing linear analogs of cyclolinopeptide A

84%
Siemion I. Z., Cebrat M., Pedyczak A., Zimecki M., Wieczorek Z.
Archivum Immunologiae et Therapiae Experimentalis
|
1993
|
tom 41
|
nr 5-6
16

The influence of transport stress on the humoral immunological response of calves induced by Mannheimia haemolytica leukotoxin

84%
Wernicki A., Urban-Chmiel R., Mikucki P., Puchalski A., Kankofer M.
Polish Journal of Veterinary Sciences
|
2003
|
tom 06
|
nr 1
In the contemporary systems of cattle production, transport stress is the most essential polyetiological factor responsible for inducing unfavourable reactions in the animals. The main reason for this phenomenon is the immunosuppressive effect of steroid hormones on cellular and humoral protective mechanisms. The purpose of the present study was to establish the relationship between the Cortisol concentration as an indicator of the stress reaction occuring directly after the transportation of calves and the specific humoral immune response to the leukotoxin (Lkt) antigen produced by the M. haemolytica strain. The experiment was carried out on 19 clinically healthy calves, weighing about 100 kg and transported by track for about 2 hours. After the delivery of the animals for feeding to the traditional cattle-house, the calves were immunized s.c.: group I with 1 ml Lkt (in conc. - 10 μg/ml) with 1 ml of adjuvant on the 1st and 14th day after the transportation, group II with the same Lkt doses on the 3rd and 16th day after the transportation. The animals of the control group were vaccinated on the 1st and 14th day after the transportation with the twice diluted adjuvant. In examined sera the Cortisol concentrations and the level of Lkt antibodies were measured by ELISA test. The cytotoxin neutralizing (CN) antibody level (cytotoxity assay) was determined with a simple visual assay. The study revealed, significant differences (P ≤ 0.01) in serum Cortisol leves between the control and experimental animals. The analysis of the absorbance of the sera in both groups immunized with Lkt showed substantial differences (P ≤ 0.05) from the 6th through to 22nd day of the experiment compared with the control group. The analysis of the results of CN antibody titers showed no differences between the sera from group I and II. Based on the results obtained in this experiment it can be assumed, that a short transportation stress has no important influence on the level of specific humoral anti-Lkt response.
17

RM-11, a new izoxasole derivative, is a potent stimulator of the humoral and cellular immune response in mice

84%
Ryng S., Sonnenberg Z., Zimecki M.
Archivum Immunologiae et Therapiae Experimentalis
|
2000
|
tom 48
|
nr 2
18

Further investigations on thymopentin-like fragments of HLA-DQ and their analogs

84%
Szewczuk Z., Wieczorek Z., Stefanowicz P., Wilczynski A., Siemion I. Z.
Archivum Immunologiae et Therapiae Experimentalis
|
1997
|
tom 45
|
nr 4
19

Influence of dimerized lysozyme (KLP-602) on the immune responses induced by furogen vaccine in rainbow trout (Oncorhynchus mykiss)

67%
Siwicki A. K., Klein P., Kazun K., Morand M., Glabski E., Trapkowska S.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2002
|
tom 05
|
nr 2
20
Content available remote

Impact of adenosine receptors on immunoglobulin production by human peripheral blood B lymphocytes

67%
Sakowicz-Burkiewicz M., Kocbuch K., Grden M., Maciejewska I., Szutowicz A., Pawelczyk T.
Journal of Physiology and Pharmacology
|
2012
|
tom 63
|
nr 6
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