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The aim of the study was to evaluate the influence of large doses of exogenous gonadotrophins on ovarian follicular growth in goats. Folliculogenesis in twelve Polish White Goats was studied in the breeding season. In every goat synchronization of estrus was realized using FGA. In group 1 (6 goats), 1200 iu of PMSG was injected at the day of sponge withdrawal (day 17). In group 2 (6 goats), for four consecutive days from day 15, 40 iu, 30 iu, 20 iu, 10 iu of FSH was injected twice a day. Daily examinations of ovaries were performed from the day when sponges were inserted, to the ovulation in the second day after superovulation estrous cycle. The number of follicles growing during medication using FGA did not differ between groups, and did not differ from observations performed during natural estrous cycle. In ovulation wave of group 1 22.2 (± 3.92) of follicles were growing, and 21 (± 3.95) reached diameter of ³ 5 mm. In ovulation wave of group 2 20.2 (± 2.93) of follicles were growing, and 18.5 (± 2.07) reached a diameter of ³ 5 mm. The differences between groups were insignificant. The ovulation rate in group 1 was 13.2 (± 3.06), and was significantly lower (P £ 0.05) than in group 2, i.e. 17.5 (± 3.67). After ovulation in the animals of group 1, follicular cysts appeared and disturbed folliculogenesis of the next estrous cycle. In the first wave there were more follicles then in the natural cycle, but after the second wave recruitment was stopped. In the ovulation wave less follicles were growing than in the natural cycle, however the ovulation rate was typical. There were larger numbers of growing follicles in the first postovulatory wave in group 2, nevertheless there were no disturbances in the rest of the cycle.
The use of exogenous steroids, including gestagens, risks several adverse effects. One of them is cystic endometrial hyperplasia (hyperplasia glandularis cystica). Estrogens stimulate the endometrial development of progesterone receptors. Progesterone and gestagens influence the hyperplasia, proliferation and secretion of endometrial glands, creating the conditions for the emergence of cysts of different sizes with partial leucocytic infiltration. Endometritis-pyometra-complex and mammary tumors are the most common pathologic findings in bitches and queens treated with gestagens. Additionally, hyperglycemia and acromegaly have been observed. These side effects are found to be mainly due to induced GH production in the mammary gland and mediated by IGF-1. The other described adverse effects are adrenocortical suppression, hepatopathy, congenital malformations, prolonged pregnancy, behavioral disorders and local alterations. In some animals in which gestagens were administrated prolactin release increases when gestagen concentration decreases.
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