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Specific identification of Escherichia coli O157 using multiplex PCR

100%
Osek J.
Bulletin of the Veterinary Institute in Puławy
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2000
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tom 44
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nr 1
A multiplex PCR was developed to identify E.coli O157 serogroup among other pathogenic and non-pathogenic E.coli serotypes as well as to differentiate them from other Gram-negative bacteria. This multiplex PCR, amplifying genes encoding the universal stress protein (uspA) and the O157 antigen-encoding operon (rfb) of E.coli, allowed a simultaneous identification of the 884 bp and 420 bp amplicons, respectively, in all O157-positive E.coli whereas no amplification products were detected in non-E.coli bacteria. The test will be useful for the rapid and specific identification of E.coli O157 bacteria responsible for human and animal diarrheal and systemic disorders.
2

The carrier state of Shiga-like toxin II [SLT II] and hemolysin-producing enteroaggregative Escherichia coli strain

86%
Sobieszczanska B. M., Gryko R., Dworniczek E., Kuzko K.
Polish Journal of Microbiology
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2004
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tom 53
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nr 2
Shiga-like toxin-producing (SLTEC) Escherichia coli strains are one of the most important food borne emerging pathogens. One hundred and fifty-seven E. coli strains isolated from 39 children with diarrhea of unknown origin and one hundred and five E. coli strains from 20 healthy children were examined for Shiga-like toxin production in Vero cell line assay. The synthesis of Shiga-like toxin was observed on Vero cell line and confirmed by PCR for one of 262 E. coli strains tested. The shiga-like toxin II-positive E. coli strain was isolated from 2-years old healthy child with no symptoms of gastrointestinal tract infection.
3

Molecular characterization of non-0157 verotoxigenic Escherichia coli isolated from slaughtered cattle in Poland

72%
Denis E., Wieczorek K., Osek J.
Medycyna Weterynaryjna
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2014
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tom 70
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nr 11
Aim of the study: The study was conducted to assess the molecular relationship of verotoxigenic E. coli (VTEC) isolated from cattle slaughtered in abattoirs in the eastern part of Poland. Materials and Methods: A total of 50 non-O157 VTEC strains isolated from bovine hides and the corresponding carcasses were tested with the pulsed field gel electrophoresis (PFGE) method. Results and discussion: The XbaI restriction analysis enabled the clustering of the isolates into 19 PFGE profiles. Seven of them contained more than two strains with two groups of 8 isolates each. Furthermore, three profiles grouped two isolates and the remaining nine PFGE types were represented by only one strain. The PFGE results were analyzed in relation to identified VTEC serotypes (O186:H16, O185:H7, O181:H49, O177:H25, O175:H21, O174:H2, O153:H25, O153:H2, O148:H8, O139:H19, O117:H4, O91:HNT, O84:H28, O36:H19, O21:H25, O2:H6, O2:H32, ONT:H34) and presence of their virulence genes. It was found that strains of the same PFGE type were usually of the same serotype and possessed the same pathogenic markers. The most numerous profile was represented by eight isolates: all of these strains were identified as O2:H32 serotype and had the same virulence genes – vtx2 and vtx2e. Furthermore, the majority of the PFGE profiles grouped the strains isolated during the same day. PFGE analysis revealed that among the isolates obtained from hides and the corresponding carcasses five pairs of strains had an identical molecular profile. The present study provided valuable information concerning the molecular characterization of VTEC isolated from cattle at the slaughter level. The results reflected a low genetic diversity among VTEC isolates tested and may suggest a common source of contamination within the abattoir.
4

The influence of yeast killer toxins on the cytotoxicity of shiga-like toxins. Part I - The effect of killer toxins on mammalian cells

72%
Salek A. T.
Biotechnologia
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2003
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nr 1
5

Recent progress of Shiga toxin neutralizer for treatment of infections by Shiga toxin-producing Escherichia coli

58%
Nishikawa K.
Archivum Immunologiae et Therapiae Experimentalis
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2011
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tom 59
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nr 4
6

Epidemiology of shiga-like toxin – producing Escherichia coli strains (STEC)

58%
Twardon J., Sobieszczanska B., Gonet A., Blaszkowska M.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
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2005
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tom 08
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nr 4
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