Wyniki wyszukiwania

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Studies on genetic transformation of tomato [Lycopersicon esculentum Mill.]

100%

Kaszuba M., Nej J., Michalczyk D. J., Pluskota W. E., Piotrowicz-Cieslak A. I., Gorecki R. J.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

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Studies on in vitro regeneration and genetic transformation of selected ornamental plants from Gesneriaceae family

88%

Sadecka I., Pluskota W. E., Michalczyk D. J., Gorecki R. J.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

3

Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis

75%

Briza J., Pavingerova D., Vlasak J., Niedermeierova H.

Acta Biochimica Polonica

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2013

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tom 60

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nr 3

Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.

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Cloning, expression and genetic transformation of sucrose phosphate synthase (SPS) gene in Saccharum spontaneum L.

75%

Acta Biologica Cracoviensia. Series Botanica

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2017

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tom 59

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nr 2

Sucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.

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Development of a rapid, reliable and simple multiplex PCR assay for early detection of transgenic plant material

75%

Xu C. J., Yang L., Chen K. S.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 3A

Multiplex PCR is a variant of conventional PCR which includes two or more pairs of primers in a single reaction to amplify corresponding genes simultaneously. In this study, a reliable multiplex PCR analysis protocol was established for simple and fast detection of transgenes in plant materials. Two pairs of primers, corresponding to neomycin phosphotransferase gene and 1-aminocyclopropane-1-carboxylate synthase gene, were selected for target and resident gene respectively. The method bypasses routine DNA extraction, requires only very little amount of plant tissue and produces reliable results as shown by successful discrimination of transformed and non transformed tobacco, tomato and kumquat materials. The method facilitates early identification of transgenic buds when they are still quite small.

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Transgenic shoots and plants as a source of natural phytochemical products

75%

Floryanowicz-Czekalska K., Wysokinska H.

Acta Societatis Botanicorum Poloniae

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2000

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tom 69

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nr 2

63%

Delis M., Garbaczewska G., Niemirowicz-Szczytt K.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 1

In vitro organogenesis in hypocotyl explants of the pepper cultivar ‘Bryza’ was induced on MS medium containing 5 mg/l 6-benzyloaminopurine (BAP) and 1 mg/l indole-3-acetic acid (IAA). The hypocotyl explants were then inoculated with Agrobacterium tumefaciens LBA4404(pBI121). After 2 days of culture the first cell divisions were observed in the epidermis and cortex. After 6-7 days, numerous adventitious bud primordia appeared in 58.4% of the explants. In further stages of culture, buds developed into shoots in 8.4% of the explants. Histological analysis revealed hypertrophy and the presence of necrotic cells in the cortex. Necrotic changes were also observed in the vascular bundles. It is likely that culture on a selective medium containing kanamycin and co-culture with Agrobacterium tumefaciens strongly affected the organization of the hypocotyl meristematic tissue, and in consequence brought about necrosis and isolation of the adventitious buds from the vascular bundles.

63%

Dale P. J.

Biological Bulletin of Poznań. Supplement

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1997

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tom 34

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Transformacja rzepaku genem reporterowym kodujacym bialko zielonej fluorescencji za pomoca Agrobacterium tumefactens.

51%

Troczynska J., Flasinski S., Drozdowska L.

Rośliny Oleiste - Oilseed Crops

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2001

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tom 22

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nr 2

569-578

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Zastosowanie metod biotechnologicznych w hodowli molekularnej rzepaku

51%

Olejniczak O., Mikolajczyk K.

Rośliny Oleiste - Oilseed Crops

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2013

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tom 34

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nr 1

W pracy przedstawiono przegląd literatury dotyczącej badań genetycznych ważnej uprawnej rośliny oleistej strefy klimatu umiarkowanego, jaką jest rzepak ozimy. Zaprezentowano metody biotechnologiczne, takie jak transformacje genetyczne z użyciem wektorów bakteryjnych oraz metodą wyciszania genów, ukierunkowana mutageneza, a także selekcja z użyciem markerów genetycznych, w odniesieniu do prowadzonych doświadczeń hodowlanych. Metody te stosowane są w celu modyfikacji i polepszenia ważnych gospodarczo cech, takich jak odporność na choroby i szkodniki, plon nasion, plon oleju, skład kwasów tłuszczowych w oleju nasion, występowanie związków aktywnych biologicznie w oleju i wytłoku oraz zawartość włókna w okrywie nasiennej. Podkreślono znaczenie identyfikacji specyficznych markerów genetycznych i ich zastosowania do selekcji w programach hodowli. Wskazano na rozwój nowej, interdyscyplinarnej dziedziny, określonej jako hodowla molekularna. Przedstawiono również praktyczne zastosowanie różnego rodzaju markerów genetycznych, identyfikujących geny i rejony genomu sprzężone z określonymi cechami, z włączeniem markerów opracowanych w IHAR – PIB, Oddział w Poznaniu.

Ograniczanie wyników

Studies on genetic transformation of tomato [Lycopersicon esculentum Mill.]

Studies on in vitro regeneration and genetic transformation of selected ornamental plants from Gesneriaceae family

Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis

Cloning, expression and genetic transformation of sucrose phosphate synthase (SPS) gene in Saccharum spontaneum L.

Development of a rapid, reliable and simple multiplex PCR assay for early detection of transgenic plant material

Transgenic shoots and plants as a source of natural phytochemical products

A simple and efficient method for triticale calli cryopreservation

The effect of L-glutamine on the genetic transformation of embryogenic cell suspensions of gentian species (Gentiana lutea L., Gentiana cruciata L., and Gentiana kurroo Royle) using Agrobacterium tumefaciens

Efficient in vitro callus induction and regeneration of different Polish cultivars of tomato

Endogenous hormone concentrations in Arabidopsis explants undergoing induction of somatic embryogenesis

Brassica oleracea var. botrytis: evaluation of in vitro potential of two commercial cultivars

Agrobacterium-mediated genetic transformation of Miscanthus sinensis and Miscanthus × giganteus. Preliminary studies

Filter paper inhibits in vitro protocorm-like body formation in hybrid Cymbidium and reduces synseed germination, but buffers the negative impact of antibiotics

Differentiation of adventitious buds from Capsicum annuum L. hypocotyls after co-culture with Agrobacterium tumefaciens

Effect of medium composition and date of explant drawing on effectiveness of Agrobacterium-mediated transformation in the petunia [Petunia hybrida pendula]

Particle-inflow-gun-mediated genetic transformation of buffel grass [Cenchrus ciliaris L.]: optimizing biological and physical parameters

Competence and determination for somatic embryogenesis and organogenesis in vitro cultured leaves of Hyacinthus orientalis L.

Potential impacts from the release of transgenic plants into the environment

Transformacja rzepaku genem reporterowym kodujacym bialko zielonej fluorescencji za pomoca Agrobacterium tumefactens.

Zastosowanie metod biotechnologicznych w hodowli molekularnej rzepaku