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Changes in DNA methylation in maize under herbicide stress conditions

100%

Tyczewska A., Gracz J., Szymkowiak J., Twardowski T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

2

Mammalian DNA methyltransferases

100%

Siedlecki P., Zielenkiewicz P.

Acta Biochimica Polonica

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2006

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tom 53

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nr 2

DNA methylation is an epigenetic process affecting gene expression and chromatin organization. It can heritably silence or activate transcription of genes without any change in their nucleotide sequences, and for a long time was not recognized as an important regulatory mechanism. However, during the recent years it has been shown that improper methylation, especially hypermethylation of promoter regions, is observed in nearly all steps of tumorigenesis. Aberrant methylation is also the cause of several major pathologies including developmental disorders involving chromosome instabilities and mental retardation. A great progress has been made in our understanding of the enzymatic machinery involved in establishing and maintaining methylation patterns. This allowed for the development of new diagnostic tools and epigenetic treatment therapies. The new approaches hold a great potential; several inhibitors of DNA methyltransferases have already shown very promising therapeutic effects.

3

Effect of some antidepressants on the low corticosterone concentration-induced gene transcription in LMCAT fibroblast cells

100%

Otczyk M., Mulik K., Budziszewska B., Jaworska-Feil L., Basta-Kaim A., Kubera M., Jagla G., Nowak W., Lason W.

Journal of Physiology and Pharmacology

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2008

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tom 59

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nr 1

The aim of the present study was to investigate effects of some classical and new antidepressants on functional activity of the glucocorticoid receceptor (GR) induced by low corticosterone concentration in mouse fibroblast cells stably transfected with mouse mammary tumor virus-chloramphenicol acetyltransferase plasmid (LMCAT cells). We found that the transcriptional activity of GR stimulated by 50 nM corticosterone was strongly attenuated by imipramine, desipramine, fluoxetine and tianeptine in a concentration-dependent way, whereas reboxetine had only a weak effect and venlafaxine was inactive. Further study revealed that the inhibitor of c-Jun N-terminal kinase - mitogen-activated protein kinase (JNK-MAPK), SP600125 (0.1 µM), reversed the imipramine-induced suppression of GR function, whereas the inhibitor of extracellular signal-regulated kinase (ERK)-MAPK, PD 98059 (15µM), potentiated the antidepressant action. No effect of selective inhibitors of p38-MAPK, phosphatidylinositol 3-kinase (PI3-K)/Akt, and glycogen synthase kinase (GSK-3) on the imipramine-induced inhibition of GR function was detected. These data indicate that the functional activity of GR evoked by low corticosterone concentration in LMCAT cells is efficiently inhibited by tricyclic antidepressants. Moreover, it was found that JNK- and ERK-MAPK were oppositely involved in the regulation of the imipramine-induced inhibition of the GR functional activity. Thus, the present study supports the notion that the interaction of antidepressants with GR may play a role in attenuating pathological hyperactivity of HPA axis in depression.

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Signal transducer and activator of transcription STAT3 plays a major role in gp130-mediated acute phase protein gene activation

100%

May P., Schniertshauer U., Gerhartz C., Horn F., Heinrich P. C.

Acta Biochimica Polonica

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2003

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tom 50

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nr 3

Interleukin-6 is a potent inducer of acute-phase response gene transcription. The intracellular signal transduction mechanisms by which this and other biological effects of the cytokine are achieved include activation of the JAK-STAT signaling pathway. More specifically, activation of the signal transducers and activators of transcription STAT1, 3, and 5 in response to IL-6 has been described. We examined the relative potency of these three STAT factors for the activation of acute-phase gene promoters in HepG2 cells in a reporter gene-based assay, where specific STAT factors could be activated via recombinant receptor constructs bearing different STAT-recruiting modules. These experiments indicate that amongst the STAT factors known to be activated by IL-6 STAT3 is the most potent activator of acute-phase gene transcription.

5

The immunoreactivity of TGF-beta1 in non-alcoholic fatty liver disease

84%

Kempinski R., Neubauer K., Poniewierka E., Kaczorowski M., Halon A.

Folia Histochemica et Cytobiologica

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2019

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tom 57

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nr 2

6

Molecular mechanisms underlying female sex determination - antagonism between female and male pathway

84%

Piprek R. P.

Folia Biologica

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2009

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tom 57

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nr 3-4

105-113

7

Cohesin Irr1/Scc3 is likely to influence transcription in Saccharomyces cerevisiae via interaction with Mediator complex

84%

Cena A., Skoneczny M., Chelstowska A., Kowalec P., Natorff R., Kurlandzka A.

Acta Biochimica Polonica

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2013

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tom 60

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nr 2

The evolutionarily conserved proteins forming sister chromatid cohesion complex are also involved in the regulation of gene transcription. The participation of SA2p (mammalian ortholog of yeast Irr1p, associated with the core of the complex) in the regulation of transcription is already described. Here we analyzed microarray profiles of gene expression of a Saccharomyces cerevisiae irr1-1/IRR1 heterozygous diploid strain. We report that expression of 33 genes is affected by the presence of the mutated Irr1-1p and identify those genes. This supports the suggested role of Irr1p in the regulation of transcription. We also indicate that Irr1p may interact with elements of transcriptional coactivator Mediator.

8

Variation in gene transcription and protein for key enzymes of carbohydrate metabolism in poplar xylem with respect to growth phase

84%

Alluvada J., Fokar M., Holaday A. S.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

We compared gene expression levels for enzymes of carbohydrate metabolism in the twig xylem of two Populus species with the seasonal levels of starch and soluble sugars (sucrose, glucose, and fructose) and relative levels of the enzymes. Plants of Populus deltoides Bartr. ex Marsh and P. balsamifera L., 3–4 years old, were grown outside in Lubbock, TX, USA in 43 L pots. The xylem in the middle portion of the twigs was sampled during the dormant period (November–February), at bud break (for P. balsamifera), and during the growth flush (April–July). The gene expression for ADP-glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy), and sucrose-phosphate synthase (SPS) generally coincided with the levels of the carbohydrates in whose metabolism these enzymes are involved. Gene expression for AGPase and its protein levels were high when the xylem starch content was high (growing period). However, P. balsamifera maintained high AGPase levels in dormant and growing twigs, unlike P. deltoides whose dormant twigs had low AGPase and low gene expression. Compared to growing twigs, gene expression for SuSy and SPS and their protein levels were higher in dormant twigs when soluble sugar content was higher. No down-regulation of these genes appears to occur when pools of the associated carbohydrates are high. Contrary to our expectation, the gene expression for bamylase was highest in growing twigs when starch content was high. High β-amylase gene expression in growing twigs may be involved in maintaining a sufficient level of soluble sugars for growth through possibly controlling the extent of starch accumulation.

9

Identification of the DNA binding element of the human ZNF300 protein

84%

Qiu H., Xue L., Gao L., Shao H., Wang D., Guo M., Li W.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 3

391-403

The human ZNF300 gene is a member of the KRAB/C2H2 zinc finger gene family, the members of which are known to be involved in various developmental and pathological processes. Here, we show that the ZNF300 gene encodes a 68-kDa nuclear protein that binds DNA in a sequence-specific manner. The ZNF300 DNA binding site, C(t/a)GGGGG(c/g)G, was defined via a random oligonucleotide selection assay, and the DNA binding site was further confirmed by electrophoretic mobility shift assays. A potential ZNF300 binding site was found in the promoter region of the human IL-2Rβ gene. The results of electrophoretic mobility shift assays indicated that ZNF300 bound to the ZNF300 binding site in the IL-2Rβ promoter in vitro. Transient co-transfection assays showed that ZNF300 could activate the IL-2Rβ promoter, and that the activation was abrogated by the mutation of residues in the ZNF300 binding site. Identifying the DNA binding site and characterizing the transcriptional regulation property of ZNF300 would provide critical insights into its potential as a transcriptional regulator.

10

Distribution of transcription factor binding sites along 5'-upstream sequences of genes: estimation of minimal promoters and upstream regulatory regions

84%

Malewski T., Glazko G., Sazanov A.

Biotechnologia

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2004

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nr 3

11

Complete nucleotide sequence of the Campylobacter jejuni 72Dz asd gene

84%

Pawelec D., Jagusztyn-Krynicka E.

Acta Microbiologica Polonica

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1996

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tom 45

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nr 3-4

12

SSCP polymorphism within 5' region of bovine lactoglobulin [LGB] gene

84%

Kaminski S., Zabolewicz T.

Journal of Applied Genetics

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1998

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tom 39

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nr 1

In the paper the detection of the SSCP polymorphism within the 5’ fragment of bovine beta-lactoglobulin (LGB) gene is described. The 5’ fragment of LGB gene (209 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 124 animals (50 cows and 74 bulls) six SSCP patterns were identified and named Rl, R2, R3, R4, R5 and R6, which occured with the frequency of 0.32, 0.51, 0.09, 0.06, 0.01 and 0.01, respectively. The PCR-SSCP method is simple, fast, and relatively inexpensive. The SSCP polymorphism reported in the paper may be useful in looking for the associations between different SSCP patterns and LGB gene expression and milk properties.

13

Redox control of cellular function by thioredoxin; a new therapeutic direction in host defence

84%

Nishinaka Y., Nakamura H., Masutani H., Yodoi J.

Archivum Immunologiae et Therapiae Experimentalis

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2001

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tom 49

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nr 4

14

Apoptosis in human polymorphonuclear leukocytes: searching for a genetic roadmap

84%

Kobayashi S. D., Deleo F. R.

Archivum Immunologiae et Therapiae Experimentalis

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2003

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tom 51

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nr 1

15

Metabolic mapping by enzyme histochemistry in living animals, tissues and cells

67%

Van Noorden C. J. F.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 4

16

Xenoestrogens: mechanisms of action and some detection studies

67%

Slomczynska M.

Polish Journal of Veterinary Sciences

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2008

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tom 11

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nr 3

263-269

Xenoestrogens are defined as chemicals that mimic some structural parts of the physiological estrogen compounds, therefore may act as estrogens or could interfere with the actions of endogenous estrogens. Two subtypes of the ER are known, the ERcc and ER ß, and both have a distinct tissue distribution and play a distinct role in physiology. Receptor dimmer assumes a distinctive conformation, binds to its estrogen response element (ERE), interacts with the general transcription complex bound to the TATA box within the respective gene promoter, and regulates gene transcription. The discovery and identification of co-activators and co-repressors provided crucial insights into the ER action. New evidence indicates that the activation of additional transcription factors as well as the action of xenoestrogens through estrogen receptors located outside the cell nucleus (in the plasma membrane, mitochondria and probably the cytosol) should be considered. The levels of exposition to xenoestrogens and the age of the investigated animal can have a significant effect on its development and reproduction. Therefore, several in vivo and in vitro assays have been developed to assess the estrogenic-like activity of individual compounds or natural mixtures. In this review, selected methods applied in physiological studies have been described. One of the most extensively used in vivo assays for estrogenicity is the rodent uterotrophic assay. In order to analyze the estrogenic properties of xenoestrogens, morphological, histological, biochemical and molecular studies should be introduced. A variety of in vitro tests have been established to determine estrogenic potency of xenoestrogens but even a combination of them is not able to predict their actual action in the organism. There is a need for the studies on all potential xenoestrogens to describe tissue-specific activities, and via which pathways in those tissues these compounds either disrupt or mimic hormone action.

17

Analysis of polymorphisms in the mediator complex subunit 13-like (MED13L) gene in the context of immune function and development of experimental arthritis

67%

Sardar S., Kanne K., Andersson A.

Archivum Immunologiae et Therapiae Experimentalis

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2018

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tom 66

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nr 5

18

Store-operated calcium entry in physiology and pathology of mammalian cells

67%

Targos B., Baranska J., Pomorski P.

Acta Biochimica Polonica

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2005

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tom 52

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nr 2

One of the numerous calcium-involving processes in mammalian cells is store-operated calcium entry (SOCE) - the process in which depletion of calcium stores in the endoplasmic reticulum (ER) induces calcium influx from the extracellular space. Previously supposed to function only in non-excitable cells, SOCE is now known to play a role also in such excitable cells as neurons, muscles and neuroendocrine cells and is found in many different cell types. SOCE participates not only in processes dependent on ER calcium level but also specifically regulates some important processes such as cAMP production, T lymphocyte activation or induction of long-term potentiation. Impairment of SOCE can be an element of numerous disorders such as acute pancreatitis, primary immunodeficiency and, since it can take part in apoptosis or cell cycle regulation, SOCE may also be partially responsible for such serious disorders as Alzheimer disease and many types of cancer. Even disturbances in the 'servant' role of maintaining ER calcium level may cause serious effects because they can lead to ER homeostasis disturbance, influencing gene expression, protein synthesis and processing, and the cell cycle.

19

Doxorubicin-induced F-actin reorganization in cofilin-1 (nonmuscle) down-regulated CHO AA8 cells

59%

Grzanka D., Marszalek A., Gagat M., Izdebska M., Gackowska L., Grzanka A.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 3

20

Ekspresja genu akwaporyny w siewkach Pharbitis nil Choisy w warunkach ciągłej ciemności

51%

Dabrowska G., Mordaka P., Prusinska J., Stawki K., Goc A.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2008

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tom 524

Kanały wodne odgrywają znaczącą rolę w rozwoju roślin i ich adaptacji do zmieniającego się środowiska zewnętrznego. Wpływ na ekspresję genów kodujących białka kanałów wodnych, akwaporyn, wywierają takie czynniki jak: hormony, susza, wysokie stężenia soli, temperatura czy światło. Gen PnPIPl, kodujący akwaporynę P. nil został zidentyfikowany w wyniku przeszukiwania biblioteki cDNA sondą - uzyskaną w wyniku reakcji różnicowego profilowania ekspresji genów. Wstępne doświadczenia sugerowały regulację ekspresji genu PnPIPl poprzez światło. Celem prezentowanej pracy było sprawdzenie poziomu transkrypcji genu akwaporyny P. nil w liścieniach siewek uprawianych w warunkach ciągłej ciemności. Stosując technikę hybrydyzacji typu northern z użyciem sondy molekularnej RNA znakowanej radioizotopowo uzyskano sygnał hybrydyzacyjny do transkryptu wielkości 1200 pz. Ekspresja genu spadała w pierwszych godzinach nocy, a wzrastała w połowie drugiej doby. Maksimum akumulacji transkryptu stwierdzono w 48 godzinie ciemności. Wyniki badań sugerują, że ekspresja genu akwaporyny PIP1 w liścieniach P. nil nie jest regulowana rytmem endogennym.

Ograniczanie wyników

Changes in DNA methylation in maize under herbicide stress conditions

Mammalian DNA methyltransferases

Effect of some antidepressants on the low corticosterone concentration-induced gene transcription in LMCAT fibroblast cells

Signal transducer and activator of transcription STAT3 plays a major role in gp130-mediated acute phase protein gene activation

The immunoreactivity of TGF-beta1 in non-alcoholic fatty liver disease

Molecular mechanisms underlying female sex determination - antagonism between female and male pathway

Cohesin Irr1/Scc3 is likely to influence transcription in Saccharomyces cerevisiae via interaction with Mediator complex

Variation in gene transcription and protein for key enzymes of carbohydrate metabolism in poplar xylem with respect to growth phase

Identification of the DNA binding element of the human ZNF300 protein

Distribution of transcription factor binding sites along 5'-upstream sequences of genes: estimation of minimal promoters and upstream regulatory regions

Complete nucleotide sequence of the Campylobacter jejuni 72Dz asd gene

SSCP polymorphism within 5' region of bovine lactoglobulin [LGB] gene

Redox control of cellular function by thioredoxin; a new therapeutic direction in host defence

Apoptosis in human polymorphonuclear leukocytes: searching for a genetic roadmap

Metabolic mapping by enzyme histochemistry in living animals, tissues and cells

Xenoestrogens: mechanisms of action and some detection studies

Analysis of polymorphisms in the mediator complex subunit 13-like (MED13L) gene in the context of immune function and development of experimental arthritis

Store-operated calcium entry in physiology and pathology of mammalian cells

Doxorubicin-induced F-actin reorganization in cofilin-1 (nonmuscle) down-regulated CHO AA8 cells

Ekspresja genu akwaporyny w siewkach Pharbitis nil Choisy w warunkach ciągłej ciemności