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Gypsophila aretioides, a cushion form evergreen plant, is a high potential wild species ideal for the use in rock garden, or as a ground cover in sunny dry areas. This plant has the competence to be developed as a new ornamental species. The purpose of this experiment was to provide an efficient micropropagation protocol for G. aretioides in order to facilitate the availability of this species for further studies of domestication. The influence of various concentrations of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) was investigated for multiplication stage. TDZ at low concentration of 0.05 mg dm−3 resulted in the maximum shoot (9.7) and leaf (42.3) number. The shoots were best rooted on MS medium containing 0.6 mg dm–3 indolebutyric acid (IBA) with 7.8 roots per shoot. Despite achievement of a successful protocol for in vitro multiplication and root induction of Gypsophila, low survival rate was obtained when rooted explants were exposed to ex vitro conditions. This is an important issue, which requires particular consideration and further studies. The possible reasons contributing to the low acclimatization rate of this species are being discussed.
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Catharanthus roseus G. Don. (CR) or periwinkle plants are widely grown/cultivated as garden plants in the tropics and subtropics. In spite of its predominantly entomophilous nature, CR pollen had been reported to be airborne and allergenic. The objective of this study was to discover the seasonal changes of CR pollen concentration in air, to determine its potential to cause respiratory allergy and to analyze its allergenic components. A 2-year aerobiological survey was conducted with a Burkard 7-day sampler in an agricultural farm in the suburban zone of Calcutta city where CR pollen was found to be almost perennial with 3.6-5.4% contribution to the aeropollen load. Skin prick test was conducted on 282 respiratory allergic individuals living within a 15 km radius of the study area. 29.8% of them were positive to CR pollen. Among them, 80.9% were directly involved in gardening. The whole pollen extract was subjected to gel fi ltration in a Sephacryl S-200 column. Among 5 eluted fractions, fraction I showed optimum IgE-reactivity in ELISA-inhibition. The fraction I shows 4 protein components in SDS-PAGE, within which 3 (40-66 kD molecular mass) were found to be IgE-reactive in immunoblotting using patient sera. It can be concluded that CR pollen can trigger IgEmediated respiratory allergy in the people living in close proximity.
Crocosmia (Crocosmia × crocosmiiflora) is an exceptionally attractive and interesting ornamental plant. Numerous varieties of this species have been produced, however, the information concerning their requirements and cultivation conditions is lacking. The study was conducted in the field conditions in the years 2008–2010. The plant material included corms of four crocosmia cultivars: ‘Emily McKenzie’, ‘Lucifer’, ‘Mars’, and ‘Meteor’. The corms were planted on 15th April, 5th May and 25th May. The number of days from the beginning of sprouting until the end of flowering was established, and measurements of vegetative and generative traits were performed during cultivation. Corm yield was determined at the end of the cultivation period. It was found that delaying the planting time resulted in accelerated sprouting of the corms. Irrespective of the cultivar, the plants grown from the corms planted on 5th May were the first, and those planted on 25th May – the last to bloom. The corm planting time affected vegetative and generative features of the crocosmia plants. The plants grown from the corms planted on 5th and 25th May were higher, had more shoots and leaves on the main shoots. The plants grown from the corms planted on 5th May were characterized by the longest main inflorescence shoots and flowers of larger diameter than the plants grown from the corms planted on 15th April and 25th May. Cultivar-specific features largely determined the vegetative and generative traits. The plants of ‘Emily McKenzie’ cultivar were characterized by the longest main inflorescence shoots and the largest flower diameter, but they produced the lowest number of inflorescence shoots and flowers per main inflorescence spike. The study showed that earlier planting time (15th April and 5th May) resulted in higher coefficient of weight and number increase of the new corms, but it did not affect the coefficient of total corm weight increase, as compared to the delayed planting time (25th May).
Introducing indigenous plants into cultivation and breeding programs in order to obtain new taxa can create the undesirable effects and pose a real threat to natural populations. The main problem of our study was to assess whether the phenotypic characters might allow distinguishing cultivated Convallaria majalis from plants occurring in natural habitats. The range of phenotype variability of Convallaria majalis L. shoots from forests and gardens growing in different edaphic factors was studied in 2005–2006 in north (Gdańsk), central (Warsaw) and south (Cracow) regions of Poland. Ten or eleven forest locations and the same number of garden locations were designated in each region. The soil at the forest sites was poorer in phosphorus and calcium and more acidic than at the garden sites from which the cultivated plants were obtained. Thirty flowering shoots were collected from each study site. The numbers as well as size of flowers, size of racemes and leaves were investigated. The following hypotheses were formulated: (a) due to conscious and unconscious selection, cultivated plants should have more numerous and larger flowers and a longer inflorescence (these characters are most significant for the ornamental appearance of the plant) than plants growing in forests; (b) cultivated plants exhibit less phenotype variability than do natural populations. The forest plants from all the three regions together had, in comparison with garden plants, significantly shorter racemes with fewer flowers, a narrower and shorter perianth, and a longer lower leaf with a narrower and longer leaf blade. In the forest plants raceme length exhibited the highest variability while the lowest was noted in perianth length. More morphological differences were noted between the forest and garden plants than among those from the three different regions. The results obtained indicate that the level of variability in the studied characters was similar in forest and cultivated plants. A high level of variability in some morphological characters of lily of the valley probably stems from the high phenotype plasticity of this plant and the diversified biotope conditions in which it occurs. The significant differences between forest and garden plants could have been related to differences in habitat conditions.
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