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An efficient system for regenerating Taraxacum pieninicum Pawl. from seedling explants

100%

Trejgell A., Chernetskyy M., Podlasiak J., Tretyn A.

Acta Biologica Cracoviensia. Series Botanica

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2013

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tom 55

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nr 1

An efficient micropropagation system for Taraxacum pieninicum using seedling explants germinated in vitro is described. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from several-day-old seedlings. The highest response, 100% frequency with 12.3 axillary shoots/explant, was from shoot tips on medium supplemented with 0.5 mg L-1 BA and 0.05 mg L-1 NAA. In subsequent subcultures the number of shoots was significantly higher on all explants cultured on medium containing 0.25 and 0.5 mg L-1 BA, and the multiplication rate was highest (20 shoots/explant) in the 4th passage. Shoots rooted on MS and 1/2 MS medium; the highest rooting frequency was 90% and the highest number of roots 2.7/shoot. Rooted plants showed 96.2% survival in sterile soil:sand, and 100% survival in hydroponic culture. Regenerated plants flowered in the second year after acclimatization and yielded viable seeds. This protocol for obtaining complete plants through micropropagation may prove useful for conservation of the genetic resources of this and other endangered species.

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Induction and subculture of callus and regeneration of fertile plants of Mesembryanthemum crystallinum L.

100%

Wang B., Luttge U.

Polish Journal of Environmental Studies

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1994

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tom 03

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nr 4

Explants of young leaves and hypocotyls of M. crystallinum were cultured on a Murashige and Skoog's (MS) medium containing auxins, cytokinins and auxin-cytokinin combinations. Callus was obtained from the medium supplemented with kinetin and 2,4-D. Callus induction was more readily obtained from hypocotyls than from leaves. Media containing zeatin (ZT), kinetin (KT), 6-benzylaminopurine (BA) and 2,4-dichlorophenoxyacetic acid (2.4-D) or a-naphthalene acetic acid (NAA) were used for bud multiplication and shoot differentiation. Shoot formation required cytokinins. The combination of cytokinins with low concentrations of auxin improved bud multiplication and shoot formation. The regenerated shoots developed roots on growth regulator free MS medium. Complete plantlets grew in the greenhouse to maturity after transfer to soil, and seeds could be collected.

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The efficiency of somatic embryogenesis of wheat [Triticum aestivum L.] I. Embryogenic reaction on young inflorescences

80%

Menke-Milczarek I., Zimny J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1992

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tom 40

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nr 3

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Bulletin of the Polish Academy of Sciences. Biological Sciences

1 Polish Journal of Environmental Studies

1 Chernetskyy M.

1 Luttge U.

1 Menke-Milczarek I.

1 Podlasiak J.

1 Trejgell A.

1 Tretyn A.

1 Wang B.

1 Zimny J.

1 2013

1 1994

1 1992

An efficient system for regenerating Taraxacum pieninicum Pawl. from seedling explants

Induction and subculture of callus and regeneration of fertile plants of Mesembryanthemum crystallinum L.

The efficiency of somatic embryogenesis of wheat [Triticum aestivum L.] I. Embryogenic reaction on young inflorescences